##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554413
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766612615505046	33.0	33.0	33.0	27.0	33.0
2	31.271775733974494	33.0	33.0	33.0	27.0	33.0
3	31.71617187908653	33.0	33.0	33.0	27.0	33.0
4	31.8399676775256	33.0	33.0	33.0	27.0	33.0
5	31.959008897699007	33.0	33.0	33.0	33.0	33.0
6	34.84420278745267	37.0	37.0	37.0	27.0	37.0
7	34.99291502904874	37.0	37.0	37.0	33.0	37.0
8	35.1659196303117	37.0	37.0	37.0	33.0	37.0
9	35.23654748355468	37.0	37.0	37.0	33.0	37.0
10	35.25475593104779	37.0	37.0	37.0	33.0	37.0
11	35.29121791877174	37.0	37.0	37.0	33.0	37.0
12	35.26418572436072	37.0	37.0	37.0	33.0	37.0
13	35.280740170234104	37.0	37.0	37.0	33.0	37.0
14	35.2580927936394	37.0	37.0	37.0	33.0	37.0
15	35.29473334860474	37.0	37.0	37.0	33.0	37.0
16	35.28726418752807	37.0	37.0	37.0	33.0	37.0
17	35.25097535591698	37.0	37.0	37.0	33.0	37.0
18	35.25734605790268	37.0	37.0	37.0	33.0	37.0
19	35.260417775196466	37.0	37.0	37.0	33.0	37.0
20	35.26674879557297	37.0	37.0	37.0	33.0	37.0
21	35.270675471174016	37.0	37.0	37.0	33.0	37.0
22	35.27263610341027	37.0	37.0	37.0	33.0	37.0
23	35.25699974567696	37.0	37.0	37.0	33.0	37.0
24	35.27021372153972	37.0	37.0	37.0	33.0	37.0
25	35.255612693064556	37.0	37.0	37.0	33.0	37.0
26	35.22000746735737	37.0	37.0	37.0	33.0	37.0
27	35.16420971369719	37.0	37.0	37.0	33.0	37.0
28	35.148315425504094	37.0	37.0	37.0	33.0	37.0
29	35.17350603250645	37.0	37.0	37.0	33.0	37.0
30	35.12889849263996	37.0	37.0	37.0	33.0	37.0
31	35.1351555609266	37.0	37.0	37.0	33.0	37.0
32	35.159002404344776	37.0	37.0	37.0	33.0	37.0
33	35.145862380571884	37.0	37.0	37.0	33.0	37.0
34	35.07906380261646	37.0	37.0	37.0	33.0	37.0
35	35.07768035742308	37.0	37.0	37.0	33.0	37.0
36	35.05571117560375	37.0	37.0	37.0	27.0	37.0
37	35.09029009060033	37.0	37.0	37.0	33.0	37.0
38	34.948846798325434	37.0	37.0	37.0	27.0	37.0
39	35.015694076437605	37.0	37.0	37.0	27.0	37.0
40	35.054131126073884	37.0	37.0	37.0	27.0	37.0
41	35.07268949321174	37.0	37.0	37.0	33.0	37.0
42	35.00279935715793	37.0	37.0	37.0	27.0	37.0
43	34.78124611075137	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	16.0
16	24.0
17	24.0
18	19.0
19	18.0
20	36.0
21	100.0
22	296.0
23	660.0
24	1549.0
25	2754.0
26	4403.0
27	6880.0
28	9561.0
29	12872.0
30	16601.0
31	21407.0
32	27014.0
33	35833.0
34	50480.0
35	92882.0
36	270984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.057118069020746	20.240867367828677	13.763025037291692	24.938989525858883
2	15.736102869160714	23.30861650069533	38.37428054536961	22.581000084774345
3	17.67887838127894	26.889881730767495	31.02344281248816	24.4077970754654
4	12.801467498056503	18.065593700003426	39.15276156944372	29.980177232496352
5	13.625402001756811	37.3155030636006	35.66150144386946	13.397593490773124
6	29.117823716254847	39.7443782883879	17.7575201158703	13.380277879486954
7	26.07099761369232	32.57409187735497	23.6435653565122	17.711345152440508
8	23.983744969905107	36.254561130420825	21.096186416985173	18.6655074826889
9	25.90379374221023	15.767848156518696	20.111541396035086	38.21681670523599
10	14.623033731171528	28.43854671517443	34.29483074891822	22.64358880473582
11	33.29268974573107	23.953803392056102	24.383988109946916	18.369518752265908
12	22.551058506925344	25.873311051508534	30.429120529280517	21.14650991228561
13	27.622909275215406	22.29853917566868	26.850019750619126	23.22853179849679
14	22.027441636469565	21.307040058584484	27.611185163407065	29.05433314153889
15	23.87371868985756	28.63460993880014	24.720019191469174	22.77165217987313
16	23.018580011651963	28.88009480297179	24.887042692000367	23.214282493375876
17	22.393955408693518	27.769550858295172	26.404864243803804	23.431629489207502
18	22.531939186130195	27.663131997265577	27.74826708608925	22.056661730514975
19	24.7335470127865	26.574593308598466	27.49430478722541	21.19755489138963
20	24.337091662713537	26.139177833131615	27.766123810228116	21.75760669392673
21	22.85011354351359	27.520819317007355	26.800778481024075	22.82828865845498
22	22.50362094683927	27.280384839460837	27.02227400872635	23.19372020497355
23	22.551058506925344	27.250082519710034	27.605413292978337	22.59344568038628
24	23.282823454716972	27.35938731595399	26.605977854054647	22.75181137527439
25	23.410526087952483	26.976459787198355	27.310687159211632	22.30232696563753
26	23.213561009572288	27.169276333707902	27.108671694206304	22.508490962513505
27	23.104977697131922	27.143844029631342	26.654317268895213	23.09686100434153
28	22.288438402418414	27.148353303403784	27.621466307608227	22.94174198656958
29	23.330802127655737	27.384819620030555	26.749192389067357	22.53518586324635
30	22.792935952079045	27.28543522608597	27.282549290871604	22.63907953096338
31	23.15584230528505	27.096586840496162	27.2827296618225	22.464841192396282
32	22.18652881516126	26.95463490213974	27.47753028879193	23.38130599390707
33	22.42948848602035	26.73313937443747	27.879396767391817	22.957975372150365
34	22.648458820410056	26.81538852804678	27.370570314909642	23.16558233663352
35	22.865625445290785	27.33936614040435	27.169817446560597	22.625190967744263
36	23.160171208106593	27.25350956777709	26.96563753014449	22.62068169397182
37	23.38888157384477	26.501903815386722	26.97736164195284	23.131852968815668
38	23.307895016891738	26.928481114259583	27.05221558657535	22.711408282273325
39	22.834782012687292	26.79699069105522	27.40195486036583	22.966272435891653
40	22.625551709646057	27.12508545073799	27.506570011886446	22.74279282772951
41	22.611302404525148	26.586497791357704	27.731312216704872	23.070887587412273
42	22.728182780706803	26.777871370260076	27.377604781994652	23.11634106703847
43	22.8645432195854	26.220705502937342	27.469413596001534	23.445337681475724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	19.5
2	28.0
3	109.5
4	191.0
5	191.0
6	231.0
7	271.0
8	251.0
9	231.0
10	341.0
11	451.0
12	451.0
13	657.5
14	864.0
15	1605.0
16	2346.0
17	2732.5
18	3119.0
19	3119.0
20	3061.0
21	3003.0
22	3668.5
23	4334.0
24	5894.0
25	7454.0
26	7454.0
27	9511.0
28	11568.0
29	15067.0
30	18566.0
31	21069.5
32	23573.0
33	23573.0
34	26453.0
35	29333.0
36	31961.0
37	34589.0
38	37737.0
39	40885.0
40	40885.0
41	42629.5
42	44374.0
43	43588.5
44	42803.0
45	42810.0
46	42817.0
47	42817.0
48	41801.0
49	40785.0
50	41611.0
51	42437.0
52	42315.0
53	42193.0
54	42193.0
55	37428.0
56	32663.0
57	28890.0
58	25117.0
59	22020.0
60	18923.0
61	18923.0
62	16639.5
63	14356.0
64	11783.5
65	9211.0
66	7814.5
67	6418.0
68	6418.0
69	5326.5
70	4235.0
71	3668.0
72	3101.0
73	2519.0
74	1937.0
75	1937.0
76	1469.0
77	1001.0
78	749.0
79	497.0
80	392.0
81	287.0
82	287.0
83	228.5
84	170.0
85	152.5
86	135.0
87	109.0
88	83.0
89	83.0
90	63.0
91	43.0
92	25.5
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	554413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.14282479119245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59088687521933	53.28106187432666
2	6.259146611502141	7.528855159959183
3	1.725697220984933	3.113649168130337
4	0.8150728418403884	1.9608313247546316
5	0.46368313036193815	1.3943606633994845
6	0.29525680822511785	1.0654547091293982
7	0.24386671504806995	1.0266783180877808
8	0.17147432058400813	0.8250360015258211
9	0.13214832002772026	0.7152995932089847
>10	1.060247186145673	12.941128008943025
>50	0.1556471462863368	6.436997973113669
>100	0.08506297327903316	8.827151924714117
>500	0.0012065669968657148	0.3884113749473435
>1k	6.032834984328574E-4	0.4950839057595985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1590	0.2867898119272095	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1139	0.20544251307238465	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.10082736155176736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.6074190179523206E-4	0.0	0.0
2	0.0	1.8037095089761603E-4	5.411128526928481E-4	0.0	0.0
3	0.0	1.8037095089761603E-4	7.214838035904641E-4	0.0	0.0
4	0.0	1.8037095089761603E-4	0.0012625966562833124	0.0	0.0
5	0.0	1.8037095089761603E-4	0.0016233385580785443	0.0	0.0
6	0.0	1.8037095089761603E-4	0.0023448223616690084	0.0	0.0
7	0.0	1.8037095089761603E-4	0.0039681609197475525	0.0	0.0
8	0.0	1.8037095089761603E-4	0.005230757576030865	0.0	0.0
9	0.0	1.8037095089761603E-4	0.007034467085007026	3.6074190179523206E-4	0.0
10	3.6074190179523206E-4	1.8037095089761603E-4	0.008838176593983185	3.6074190179523206E-4	0.0
11	3.6074190179523206E-4	1.8037095089761603E-4	0.013347450366423586	5.411128526928481E-4	0.0
12	3.6074190179523206E-4	1.8037095089761603E-4	0.014970788924502132	5.411128526928481E-4	0.0
13	3.6074190179523206E-4	1.8037095089761603E-4	0.01587264367899021	5.411128526928481E-4	0.0
14	3.6074190179523206E-4	1.8037095089761603E-4	0.01731561128617114	5.411128526928481E-4	0.0
15	3.6074190179523206E-4	1.8037095089761603E-4	0.018758578893352067	5.411128526928481E-4	0.0
16	3.6074190179523206E-4	1.8037095089761603E-4	0.019840804598737764	5.411128526928481E-4	0.0
17	5.411128526928481E-4	1.8037095089761603E-4	0.021103401255021078	5.411128526928481E-4	0.0
18	5.411128526928481E-4	1.8037095089761603E-4	0.02128377220591869	7.214838035904641E-4	0.0
19	5.411128526928481E-4	1.8037095089761603E-4	0.021644514107713925	9.018547544880802E-4	0.0
20	5.411128526928481E-4	1.8037095089761603E-4	0.02182488505861154	9.018547544880802E-4	0.0
21	5.411128526928481E-4	1.8037095089761603E-4	0.02326785266579247	0.0014429676071809282	0.0
22	5.411128526928481E-4	1.8037095089761603E-4	0.023628594567587702	0.0016233385580785443	0.0
23	5.411128526928481E-4	1.8037095089761603E-4	0.023989336469382932	0.0019840804598737763	0.0
24	5.411128526928481E-4	1.8037095089761603E-4	0.023989336469382932	0.0025251933125666247	0.0
25	5.411128526928481E-4	1.8037095089761603E-4	0.023989336469382932	0.0030663061652594728	0.0
26	5.411128526928481E-4	1.8037095089761603E-4	0.023989336469382932	0.0030663061652594728	0.0
27	5.411128526928481E-4	1.8037095089761603E-4	0.02416970742028055	0.0039681609197475525	0.0
28	5.411128526928481E-4	1.8037095089761603E-4	0.02416970742028055	0.010100773250266499	0.0
29	5.411128526928481E-4	1.8037095089761603E-4	0.02416970742028055	0.027055642634642406	0.0
30	5.411128526928481E-4	1.8037095089761603E-4	0.02416970742028055	0.050323495300434874	0.0
31	5.411128526928481E-4	1.8037095089761603E-4	0.02416970742028055	0.12211113375768606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCA	95	0.0	27.263159	12
TCGCCAT	100	0.0	25.900002	13
CGAGTCG	50	9.080006E-6	25.900002	21
GTATCAA	1175	0.0	25.03404	1
AGGACCG	45	1.3220053E-4	24.666668	5
CGCTAAT	45	1.3220053E-4	24.666668	32
GGTATCA	385	0.0	23.545454	1
TAGACCT	40	0.0019299331	23.125	4
GCCGTCC	50	2.6996576E-4	22.2	27
ACGTGGG	145	0.0	21.689655	36
CGAGACA	60	3.7218095E-5	21.583332	23
GGCCGCA	115	1.3460522E-10	20.913044	33
AGAGGTC	150	0.0	19.733332	8
CGTGGGA	160	0.0	19.65625	37
GCACCGC	95	1.6722151E-7	19.473684	10
CGGCCTT	105	2.2531822E-8	19.38095	24
TCATACT	105	2.2531822E-8	19.38095	4
TTGGCCG	125	4.110916E-10	19.24	31
TGGCCGC	125	4.110916E-10	19.24	32
GCCACGT	165	0.0	19.060606	33
>>END_MODULE