FastQCFastQC Report
Fri 10 Feb 2017
ERR1633050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512378
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13550.26445319666340084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.17740808543692352No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6330.12354160405013487No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6090.11885756219041411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5140.10031656316235278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3150.026.428571
CGTTCGC502.69927E-422.234
GTATCAA8050.022.0621131
TGCACGC603.721098E-521.58333437
GTTATTC555.1377487E-420.1818183
GGATATA656.89259E-519.9230771
ACTTATG656.89259E-519.92307732
ACCTATA656.89259E-519.9230771
GCGGGTA656.89259E-519.92307722
CCTCTAG1551.8189894E-1219.0967751
CGGGTAA609.2272897E-418.523
TTAGATA701.21752455E-418.54
TGCGGTA609.2272897E-418.536
ACGGGCT701.21752455E-418.516
GGTATAT500.0070296918.51
ACGTCAA500.0070296918.529
TATACTG701.21752455E-418.55
ATGCACG801.6138223E-518.536
AATACTG2700.017.8148165
GTTAACT953.5984995E-617.52631628
Produced by FastQC (version 0.11.5)