##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512378
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76121730441198	33.0	33.0	33.0	27.0	33.0
2	31.286243749731643	33.0	33.0	33.0	27.0	33.0
3	31.723893297526434	33.0	33.0	33.0	27.0	33.0
4	31.847237781481642	33.0	33.0	33.0	27.0	33.0
5	31.946512145330207	33.0	33.0	33.0	33.0	33.0
6	34.85608281386008	37.0	37.0	37.0	27.0	37.0
7	35.01758077044682	37.0	37.0	37.0	33.0	37.0
8	35.16521201144467	37.0	37.0	37.0	33.0	37.0
9	35.27657901002775	37.0	37.0	37.0	33.0	37.0
10	35.26972274375559	37.0	37.0	37.0	33.0	37.0
11	35.30726924262947	37.0	37.0	37.0	33.0	37.0
12	35.27832186393639	37.0	37.0	37.0	33.0	37.0
13	35.30670520592219	37.0	37.0	37.0	33.0	37.0
14	35.27584517680307	37.0	37.0	37.0	33.0	37.0
15	35.31780833681383	37.0	37.0	37.0	33.0	37.0
16	35.31039193720261	37.0	37.0	37.0	33.0	37.0
17	35.28103665653092	37.0	37.0	37.0	33.0	37.0
18	35.27981685396328	37.0	37.0	37.0	33.0	37.0
19	35.27563829828759	37.0	37.0	37.0	33.0	37.0
20	35.27556998934381	37.0	37.0	37.0	33.0	37.0
21	35.288882426645955	37.0	37.0	37.0	33.0	37.0
22	35.27366709733829	37.0	37.0	37.0	33.0	37.0
23	35.27756656218651	37.0	37.0	37.0	33.0	37.0
24	35.274518031609475	37.0	37.0	37.0	33.0	37.0
25	35.279892969643505	37.0	37.0	37.0	33.0	37.0
26	35.23304279262576	37.0	37.0	37.0	33.0	37.0
27	35.18133292217855	37.0	37.0	37.0	33.0	37.0
28	35.16879530346736	37.0	37.0	37.0	33.0	37.0
29	35.18648536822424	37.0	37.0	37.0	33.0	37.0
30	35.15177661804371	37.0	37.0	37.0	33.0	37.0
31	35.14777761730598	37.0	37.0	37.0	33.0	37.0
32	35.167153937132355	37.0	37.0	37.0	33.0	37.0
33	35.17176576668007	37.0	37.0	37.0	33.0	37.0
34	35.10126312995484	37.0	37.0	37.0	33.0	37.0
35	35.08871575282311	37.0	37.0	37.0	33.0	37.0
36	35.07984534855127	37.0	37.0	37.0	33.0	37.0
37	35.106885931870615	37.0	37.0	37.0	33.0	37.0
38	34.97523312866673	37.0	37.0	37.0	27.0	37.0
39	35.046602313136006	37.0	37.0	37.0	27.0	37.0
40	35.073197912478676	37.0	37.0	37.0	33.0	37.0
41	35.0822517750567	37.0	37.0	37.0	33.0	37.0
42	35.01824239135951	37.0	37.0	37.0	27.0	37.0
43	34.80359812482191	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	20.0
17	30.0
18	26.0
19	21.0
20	38.0
21	93.0
22	286.0
23	694.0
24	1375.0
25	2366.0
26	3948.0
27	6102.0
28	8568.0
29	11628.0
30	15401.0
31	19725.0
32	25179.0
33	32745.0
34	46588.0
35	86401.0
36	251132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.963897747366204	20.40466218299771	13.446908337204174	25.184531732431914
2	16.08968378814079	22.702184715190736	38.03539574298662	23.172735753681852
3	17.727732260167297	26.50230884230002	30.221242910507478	25.548715987025204
4	12.74761992123003	17.819266244842673	38.87852327773636	30.554590556190934
5	14.080815335553048	36.82671777476785	35.37934884011413	13.71311804956497
6	29.91678018962563	39.68827701423558	17.238054717415658	13.156888078723131
7	26.650051329292047	32.19595689120142	23.4403116449184	17.713680134588135
8	24.2885135583495	35.9150861278197	20.668529874428646	19.127870439402162
9	25.97750098560048	15.427672538633585	20.065264316578777	38.529562159187165
10	14.974491488705604	27.818719773292372	34.1197319166709	23.087056821331124
11	34.38633196585334	23.24475289727506	24.23698909789257	18.131926038979035
12	23.34721631295645	25.496996358157453	30.10999691633911	21.045790412546985
13	27.86399884460301	22.210165151509237	25.86176611798321	24.064069885904544
14	22.091307589318824	20.840863581184205	27.299376632095836	29.768452197401135
15	24.101737389193133	28.384903333086122	24.310762757183173	23.202596520537572
16	23.280859053277073	28.217448836601104	24.81117456253001	23.690517547591817
17	22.68618090550336	27.33801997743853	26.230634414436217	23.745164702621892
18	23.20357236259168	27.2954732638794	27.308744715815276	22.19220965771364
19	24.932959650882747	26.262837202221796	26.676594233163797	22.12760891373166
20	24.37360698546776	26.30577425260257	27.051317581941458	22.26930117998821
21	23.4861762214615	26.842877719183882	26.910601157739013	22.760344901615603
22	23.000987552158758	26.80364886860872	26.845024571702925	23.3503390075296
23	23.07261435893032	26.843072887594705	27.311867410388423	22.77244534308655
24	23.627087814074766	27.109477768366325	25.73802934552225	23.52540507203666
25	23.51018193599257	26.832143456588692	26.918798230993524	22.738876376425218
26	23.580052227066737	27.039607477292154	26.734949587999484	22.645390707641624
27	23.468025559255082	26.94319428234624	26.286452579931225	23.30232757846746
28	22.957464996545518	26.598917205656765	27.20315860556074	23.24045919223698
29	24.10466491535546	26.67249569653654	26.422484962273945	22.80035442583405
30	23.839626213459596	26.37662038573085	26.922701599209958	22.8610518015996
31	23.634309045275167	26.415458899484367	26.69669657947843	23.253535475762035
32	22.527508987505318	26.53587780896135	27.135630335416433	23.8009828681169
33	22.808551499088566	26.683620295953382	27.426626435951583	23.081201769006476
34	23.324186440479487	26.37252184910359	27.21525904703168	23.088032663385235
35	23.36829450132519	27.02672636217792	26.748806545167824	22.856172591329056
36	24.043772371179088	26.515385125825077	26.50074749501345	22.94009500798239
37	23.35072934435124	26.154323565804933	26.7899870798512	23.70496000999262
38	23.522672714285157	26.587597437829103	26.45839594986514	23.4313338980206
39	23.097595915515498	26.478888633001418	27.03082489880518	23.392690552677905
40	23.158098122870225	27.06380836023405	27.0517079187631	22.72638559813263
41	23.2630987278923	26.064936433648594	27.144998419135874	23.526966419323234
42	22.952780954685796	26.89537802169492	27.011893562955475	23.139947460663805
43	23.575368185207015	25.831319845895024	27.058343644731035	23.534968324166925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	12.0
2	20.0
3	71.0
4	122.0
5	122.0
6	157.0
7	192.0
8	185.0
9	178.0
10	238.0
11	298.0
12	298.0
13	462.5
14	627.0
15	1142.5
16	1658.0
17	2023.5
18	2389.0
19	2389.0
20	2296.5
21	2204.0
22	2638.5
23	3073.0
24	4199.0
25	5325.0
26	5325.0
27	7300.5
28	9276.0
29	11493.0
30	13710.0
31	16652.0
32	19594.0
33	19594.0
34	22859.5
35	26125.0
36	28673.0
37	31221.0
38	34264.0
39	37307.0
40	37307.0
41	39083.0
42	40859.0
43	42078.0
44	43297.0
45	42654.0
46	42011.0
47	42011.0
48	40657.5
49	39304.0
50	39048.5
51	38793.0
52	36982.0
53	35171.0
54	35171.0
55	33317.5
56	31464.0
57	27727.5
58	23991.0
59	22185.0
60	20379.0
61	20379.0
62	17705.5
63	15032.0
64	12560.5
65	10089.0
66	8441.0
67	6793.0
68	6793.0
69	5781.5
70	4770.0
71	3843.5
72	2917.0
73	2387.5
74	1858.0
75	1858.0
76	1409.0
77	960.0
78	755.5
79	551.0
80	433.5
81	316.0
82	316.0
83	259.0
84	202.0
85	189.0
86	176.0
87	128.5
88	81.0
89	81.0
90	56.5
91	32.0
92	20.0
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	512378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.60949932261766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71842063888911	53.42678477610112
2	7.290312368696428	8.983049898817475
3	2.1553783064995757	3.983753349428112
4	1.015407956879499	2.5023510332619225
5	0.5661801324326472	1.7441037242794377
6	0.4033632090947829	1.4910603214496345
7	0.271495223723535	1.1708679361462342
8	0.19971956325549362	0.9843697837682264
9	0.17330333179472057	0.9609418352532799
>10	1.0351158089004098	12.376526042434506
>50	0.10846029152704152	4.633505167919637
>100	0.060929163180699644	6.854152168329869
>500	0.0015950042717460633	0.6222292692453723
>1k	3.190008543492127E-4	0.2663046935652178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1355	0.26445319666340084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	909	0.17740808543692352	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	633	0.12354160405013487	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	609	0.11885756219041411	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.10031656316235278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.903368216433961E-4	0.0	0.0
2	0.0	0.0	3.903368216433961E-4	0.0	0.0
3	0.0	0.0	5.855052324650941E-4	0.0	0.0
4	0.0	0.0	5.855052324650941E-4	1.9516841082169804E-4	0.0
5	0.0	0.0	5.855052324650941E-4	3.903368216433961E-4	0.0
6	0.0	0.0	7.806736432867922E-4	3.903368216433961E-4	0.0
7	0.0	0.0	0.0021468525190386787	3.903368216433961E-4	0.0
8	0.0	0.0	0.0035130313947905646	3.903368216433961E-4	1.9516841082169804E-4
9	0.0	0.0	0.004684041859720753	3.903368216433961E-4	1.9516841082169804E-4
10	7.806736432867922E-4	0.0	0.005269547092185847	3.903368216433961E-4	1.9516841082169804E-4
11	7.806736432867922E-4	0.0	0.007611568022046223	3.903368216433961E-4	1.9516841082169804E-4
12	7.806736432867922E-4	0.0	0.007806736432867922	3.903368216433961E-4	3.903368216433961E-4
13	7.806736432867922E-4	0.0	0.008587410076154715	3.903368216433961E-4	3.903368216433961E-4
14	7.806736432867922E-4	0.0	0.009368083719441506	5.855052324650941E-4	3.903368216433961E-4
15	7.806736432867922E-4	0.0	0.010148757362728298	5.855052324650941E-4	3.903368216433961E-4
16	7.806736432867922E-4	0.0	0.01190527306012358	5.855052324650941E-4	3.903368216433961E-4
17	7.806736432867922E-4	0.0	0.01210044147094528	5.855052324650941E-4	3.903368216433961E-4
18	7.806736432867922E-4	0.0	0.01210044147094528	5.855052324650941E-4	5.855052324650941E-4
19	7.806736432867922E-4	0.0	0.01210044147094528	5.855052324650941E-4	5.855052324650941E-4
20	7.806736432867922E-4	0.0	0.012295609881766976	5.855052324650941E-4	5.855052324650941E-4
21	7.806736432867922E-4	0.0	0.012685946703410373	7.806736432867922E-4	5.855052324650941E-4
22	7.806736432867922E-4	0.0	0.012685946703410373	7.806736432867922E-4	5.855052324650941E-4
23	7.806736432867922E-4	0.0	0.01288111511423207	9.758420541084902E-4	5.855052324650941E-4
24	7.806736432867922E-4	0.0	0.01288111511423207	9.758420541084902E-4	5.855052324650941E-4
25	7.806736432867922E-4	0.0	0.01288111511423207	0.0011710104649301882	5.855052324650941E-4
26	7.806736432867922E-4	0.0	0.013076283525053769	0.0013661788757518864	5.855052324650941E-4
27	7.806736432867922E-4	0.0	0.013076283525053769	0.0025371893406820746	7.806736432867922E-4
28	7.806736432867922E-4	0.0	0.013076283525053769	0.005659883913829243	7.806736432867922E-4
29	7.806736432867922E-4	0.0	0.013076283525053769	0.015808641276557543	7.806736432867922E-4
30	7.806736432867922E-4	0.0	0.013076283525053769	0.030251103677363198	7.806736432867922E-4
31	7.806736432867922E-4	0.0	0.013076283525053769	0.0893871321563377	7.806736432867922E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	315	0.0	26.42857	1
CGTTCGC	50	2.69927E-4	22.2	34
GTATCAA	805	0.0	22.062113	1
TGCACGC	60	3.721098E-5	21.583334	37
GTTATTC	55	5.1377487E-4	20.181818	3
GGATATA	65	6.89259E-5	19.923077	1
ACTTATG	65	6.89259E-5	19.923077	32
ACCTATA	65	6.89259E-5	19.923077	1
GCGGGTA	65	6.89259E-5	19.923077	22
CCTCTAG	155	1.8189894E-12	19.096775	1
CGGGTAA	60	9.2272897E-4	18.5	23
TTAGATA	70	1.21752455E-4	18.5	4
TGCGGTA	60	9.2272897E-4	18.5	36
ACGGGCT	70	1.21752455E-4	18.5	16
GGTATAT	50	0.00702969	18.5	1
ACGTCAA	50	0.00702969	18.5	29
TATACTG	70	1.21752455E-4	18.5	5
ATGCACG	80	1.6138223E-5	18.5	36
AATACTG	270	0.0	17.814816	5
GTTAACT	95	3.5984995E-6	17.526316	28
>>END_MODULE