FastQCFastQC Report
Fri 10 Feb 2017
ERR1633049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523425
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14820.2831351196446482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.1841715623059655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT250.00549364329.5999985
GTATCAA10050.024.4825861
GGTATCA3400.020.676471
CAGTTAG450.003823066120.5555551
TTTATAC909.45347E-820.5555553
CGAGACG450.003823066120.55555523
TAACGCT555.137951E-420.18181814
ATCTCCG656.892953E-519.92307710
GTATTAG2150.018.9302331
ATTAATC609.227656E-418.53
ATTAACG609.227656E-418.512
AACGGCC1103.8397047E-818.537
ACGCTTG609.227656E-418.516
AATTCCG701.2175883E-418.55
TATACAC1601.8189894E-1218.53
ATTAGAG1351.1477823E-917.8148163
GCACCGC1258.547431E-917.7610
ACACTTA852.7185306E-517.4117646
TATTAGA1603.45608E-1117.343752
GGGCTAT752.0645572E-417.2666661
Produced by FastQC (version 0.11.5)