##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523425
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.767410803840093	33.0	33.0	33.0	27.0	33.0
2	31.28515260065912	33.0	33.0	33.0	27.0	33.0
3	31.717684482017482	33.0	33.0	33.0	27.0	33.0
4	31.84610020537804	33.0	33.0	33.0	27.0	33.0
5	31.95102450207766	33.0	33.0	33.0	33.0	33.0
6	34.86034484405598	37.0	37.0	37.0	27.0	37.0
7	35.02684434255146	37.0	37.0	37.0	33.0	37.0
8	35.186681950613746	37.0	37.0	37.0	33.0	37.0
9	35.270858289153175	37.0	37.0	37.0	33.0	37.0
10	35.299555810288005	37.0	37.0	37.0	33.0	37.0
11	35.3114123322348	37.0	37.0	37.0	33.0	37.0
12	35.289080575058506	37.0	37.0	37.0	33.0	37.0
13	35.31427043033864	37.0	37.0	37.0	33.0	37.0
14	35.282428237092226	37.0	37.0	37.0	33.0	37.0
15	35.3021407078378	37.0	37.0	37.0	33.0	37.0
16	35.30052634092755	37.0	37.0	37.0	33.0	37.0
17	35.271675980321916	37.0	37.0	37.0	33.0	37.0
18	35.27180398337871	37.0	37.0	37.0	33.0	37.0
19	35.281879925490756	37.0	37.0	37.0	33.0	37.0
20	35.27202560061136	37.0	37.0	37.0	33.0	37.0
21	35.27200076419735	37.0	37.0	37.0	33.0	37.0
22	35.2840406935091	37.0	37.0	37.0	33.0	37.0
23	35.257452357071216	37.0	37.0	37.0	33.0	37.0
24	35.264568944930026	37.0	37.0	37.0	33.0	37.0
25	35.256086354301	37.0	37.0	37.0	33.0	37.0
26	35.23435449204757	37.0	37.0	37.0	33.0	37.0
27	35.17331613889287	37.0	37.0	37.0	33.0	37.0
28	35.14830778048431	37.0	37.0	37.0	33.0	37.0
29	35.186601709891576	37.0	37.0	37.0	33.0	37.0
30	35.13871901418541	37.0	37.0	37.0	33.0	37.0
31	35.134496823804746	37.0	37.0	37.0	33.0	37.0
32	35.16658547069781	37.0	37.0	37.0	33.0	37.0
33	35.14173950422697	37.0	37.0	37.0	33.0	37.0
34	35.074102306920764	37.0	37.0	37.0	33.0	37.0
35	35.06841858910064	37.0	37.0	37.0	27.0	37.0
36	35.04945885274872	37.0	37.0	37.0	27.0	37.0
37	35.08145388546592	37.0	37.0	37.0	33.0	37.0
38	34.935096718727614	37.0	37.0	37.0	27.0	37.0
39	35.018715193198645	37.0	37.0	37.0	27.0	37.0
40	35.054133830061616	37.0	37.0	37.0	27.0	37.0
41	35.072447819649426	37.0	37.0	37.0	33.0	37.0
42	34.99351578545159	37.0	37.0	37.0	27.0	37.0
43	34.78486125041792	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	29.0
17	22.0
18	19.0
19	24.0
20	34.0
21	94.0
22	230.0
23	667.0
24	1389.0
25	2495.0
26	4181.0
27	6292.0
28	8973.0
29	12037.0
30	15574.0
31	20104.0
32	25473.0
33	33706.0
34	48113.0
35	88538.0
36	255417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02211396093041	20.278358886182357	13.524955819840473	25.174571333046757
2	16.05311171610068	22.7690691120982	37.72918756268807	23.448631609113054
3	17.81936285045613	26.74690738883317	29.89177054974447	25.54195921096623
4	12.850742704303386	17.878397096050055	38.97502029899221	30.295839900654343
5	14.11778191718011	36.98447724124755	35.15154988775851	13.746190953813823
6	29.959975163585995	39.62076706309404	17.297798156373883	13.121459616946076
7	26.881024024454312	31.942494149114008	23.51836461766251	17.658117208769163
8	24.585566222476952	35.93485217557434	20.493671490662464	18.985910111286238
9	25.987295218990305	15.456082533314227	20.099536705354158	38.457085542341304
10	14.953240674404164	28.10889812294025	33.88623011892821	23.051631083727372
11	34.31933896928882	23.471557529732053	24.027320055404306	18.18178344557482
12	23.151740937097003	25.498591011128624	30.132683765582463	21.21698428619191
13	28.0481444332999	22.13612265367531	25.88030758943497	23.93542532358982
14	22.066389645125852	20.743755074748055	27.38061804461002	29.809237235516072
15	24.008406170893632	28.57238381812103	24.15914409896356	23.26006591202178
16	23.30610880259827	28.229450255528487	24.936714906624637	23.5277260352486
17	22.74480584610976	27.396093041027846	26.2257247934279	23.633376319434493
18	23.350432249128335	27.199312222381426	27.298466829058604	22.15178869943163
19	24.984477241247554	26.18331184028275	26.78893824330133	22.043272675168364
20	24.39833787075512	26.21750967187276	27.072646510961455	22.311505946410662
21	23.513779433538712	26.810144719873907	26.876247790992025	22.79982805559536
22	23.08124373119358	26.757224053111717	26.95610641448154	23.205425801213163
23	23.155752973205328	26.54286669532407	27.445765869035675	22.85561446243492
24	23.21879925490758	27.109519033290347	26.03352915890529	23.638152552896784
25	23.49161771027368	26.712518507904665	27.090987247456656	22.704876534365003
26	23.50327171992167	26.897454267564598	26.836318479247268	22.762955533266467
27	23.23465635000239	26.949228638295843	26.476381525528968	23.339733486172804
28	22.999092515642165	26.66647561732817	27.244781964942444	23.089649902087213
29	24.117495343172372	26.667812962697617	26.401490184840238	22.813201509289772
30	23.619620767063093	26.464536466542484	27.02564837369251	22.890194392701915
31	23.620002865740076	26.433395424368346	26.865166929359507	23.081434780532074
32	22.58222285905335	26.427854993552085	27.18058938720925	23.809332760185317
33	22.781678368438648	26.529111142952665	27.579118307302863	23.110092181305824
34	23.211539380044897	26.294502555284904	27.18441037397908	23.309547690691122
35	23.488751970196304	26.9547690691121	26.7346802311697	22.8217987295219
36	24.014519749725366	26.65348426231074	26.35697568897168	22.975020298992217
37	23.24057887949563	26.241772937861203	26.733533935138748	23.78411424750442
38	23.42761618187897	26.601136743564023	26.73811911926255	23.233127955294457
39	23.055643119835697	26.510006209103498	27.05602521851268	23.378325452548122
40	23.008071834551274	26.95954530257439	27.243253570234515	22.789129292639824
41	23.158427663944213	25.993790896499018	27.274585661747146	23.57319577780962
42	22.81052681855089	26.913502411997897	27.0648134880833	23.211157281367914
43	23.481109996656638	25.911066532932132	27.002722453073506	23.605101017337727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	18.5
2	27.0
3	87.0
4	147.0
5	147.0
6	189.5
7	232.0
8	207.5
9	183.0
10	253.5
11	324.0
12	324.0
13	502.0
14	680.0
15	1256.0
16	1832.0
17	2232.5
18	2633.0
19	2633.0
20	2515.5
21	2398.0
22	2863.5
23	3329.0
24	4635.0
25	5941.0
26	5941.0
27	7804.5
28	9668.0
29	12114.0
30	14560.0
31	17561.5
32	20563.0
33	20563.0
34	23843.0
35	27123.0
36	29860.0
37	32597.0
38	35218.0
39	37839.0
40	37839.0
41	39658.5
42	41478.0
43	42122.5
44	42767.0
45	42014.5
46	41262.0
47	41262.0
48	40184.0
49	39106.0
50	38614.0
51	38122.0
52	36552.0
53	34982.0
54	34982.0
55	33783.5
56	32585.0
57	28681.5
58	24778.0
59	22766.0
60	20754.0
61	20754.0
62	18315.5
63	15877.0
64	13474.0
65	11071.0
66	9361.0
67	7651.0
68	7651.0
69	6335.0
70	5019.0
71	4144.5
72	3270.0
73	2621.0
74	1972.0
75	1972.0
76	1531.5
77	1091.0
78	839.5
79	588.0
80	492.0
81	396.0
82	396.0
83	312.5
84	229.0
85	204.5
86	180.0
87	141.5
88	103.0
89	103.0
90	78.0
91	53.0
92	28.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	523425.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.75502654714543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.00713710112487	57.86911636422734
2	6.991183255530389	9.194108811267188
3	1.8105562083743982	3.5715951464027262
4	0.8489392136798573	2.2328808212972713
5	0.48247001136877626	1.586241420287772
6	0.3264682596128515	1.2880157446586051
7	0.2309182725645452	1.0628825999891842
8	0.1536943722914555	0.8084942024137209
9	0.13235086782725755	0.7832461344767151
>10	0.8763438319302463	11.000904312542772
>50	0.09280447425068691	4.265492309848516
>100	0.04625585570346428	5.766441811163164
>500	5.855171608033412E-4	0.28529016071252794
>1k	2.927585804016706E-4	0.28529016071252794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1482	0.2831351196446482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	964	0.1841715623059655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.731480154749964E-4	0.0	0.0
2	0.0	0.0	5.731480154749964E-4	0.0	0.0
3	0.0	0.0	5.731480154749964E-4	0.0	0.0
4	0.0	0.0	5.731480154749964E-4	0.0	0.0
5	0.0	0.0	7.641973539666619E-4	0.0	0.0
6	0.0	0.0	0.0021015427234083203	0.0	0.0
7	0.0	0.0	0.002865740077374982	0.0	0.0
8	0.0	0.0	0.0034388880928499783	1.9104933849166546E-4	0.0
9	0.0	0.0	0.0038209867698333093	5.731480154749964E-4	0.0
10	5.731480154749964E-4	0.0	0.004012036108324975	5.731480154749964E-4	0.0
11	5.731480154749964E-4	0.0	0.0063046281702249605	5.731480154749964E-4	0.0
12	5.731480154749964E-4	0.0	0.006686726847208292	7.641973539666619E-4	0.0
13	5.731480154749964E-4	0.0	0.0076419735396666185	7.641973539666619E-4	0.0
14	5.731480154749964E-4	0.0	0.008788269570616613	7.641973539666619E-4	0.0
15	5.731480154749964E-4	0.0	0.009361417586091608	9.552466924583273E-4	0.0
16	7.641973539666619E-4	0.0	0.01012561494005827	9.552466924583273E-4	0.0
17	7.641973539666619E-4	0.0	0.011080861632516597	0.0011462960309499928	0.0
18	7.641973539666619E-4	0.0	0.011271910971008263	0.0011462960309499928	0.0
19	7.641973539666619E-4	0.0	0.011654009647991594	0.0011462960309499928	0.0
20	7.641973539666619E-4	0.0	0.01184505898648326	0.0013373453694416582	0.0
21	7.641973539666619E-4	0.0	0.012036108324974924	0.0013373453694416582	0.0
22	7.641973539666619E-4	0.0	0.012036108324974924	0.0015283947079333237	0.0
23	7.641973539666619E-4	0.0	0.012036108324974924	0.0019104933849166546	0.0
24	7.641973539666619E-4	0.0	0.01222715766346659	0.0019104933849166546	0.0
25	7.641973539666619E-4	0.0	0.01222715766346659	0.0021015427234083203	0.0
26	7.641973539666619E-4	0.0	0.01222715766346659	0.0022925920618999856	0.0
27	7.641973539666619E-4	0.0	0.01222715766346659	0.0030567894158666474	0.0
28	7.641973539666619E-4	0.0	0.01222715766346659	0.007833022878158284	0.0
29	7.641973539666619E-4	0.0	0.01222715766346659	0.017958637818216553	0.0
30	7.641973539666619E-4	0.0	0.01222715766346659	0.034770979605483116	0.0
31	7.641973539666619E-4	0.0	0.01222715766346659	0.08998423842957444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTT	25	0.005493643	29.599998	5
GTATCAA	1005	0.0	24.482586	1
GGTATCA	340	0.0	20.67647	1
CAGTTAG	45	0.0038230661	20.555555	1
TTTATAC	90	9.45347E-8	20.555555	3
CGAGACG	45	0.0038230661	20.555555	23
TAACGCT	55	5.137951E-4	20.181818	14
ATCTCCG	65	6.892953E-5	19.923077	10
GTATTAG	215	0.0	18.930233	1
ATTAATC	60	9.227656E-4	18.5	3
ATTAACG	60	9.227656E-4	18.5	12
AACGGCC	110	3.8397047E-8	18.5	37
ACGCTTG	60	9.227656E-4	18.5	16
AATTCCG	70	1.2175883E-4	18.5	5
TATACAC	160	1.8189894E-12	18.5	3
ATTAGAG	135	1.1477823E-9	17.814816	3
GCACCGC	125	8.547431E-9	17.76	10
ACACTTA	85	2.7185306E-5	17.411764	6
TATTAGA	160	3.45608E-11	17.34375	2
GGGCTAT	75	2.0645572E-4	17.266666	1
>>END_MODULE