FastQCFastQC Report
Fri 10 Feb 2017
ERR1633048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577625
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29630.5129625622159706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.3606145855875352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11050.19130058428911492No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8030.1390175286734473No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7560.13088076173988314No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7460.12914953473274182No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7220.1249945899156027No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6380.11045228305561568No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5820.10075741181562432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.1000649210127678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT1350.026.03703728
GCCGCTC1400.025.10714327
CGCTCTC1400.025.10714329
GGTATCA6700.024.0223881
TTCGCCG1550.022.67741824
GCCGGCA1500.022.215
GCACCGC1005.329639E-1022.19999910
TGCTCGC1700.021.76470610
GCTTCGC1650.021.3030322
GTATCAA14900.020.9832211
GGTCGGC555.138838E-420.18181837
CGGCAGC1750.020.08571417
TATTAGA1600.019.656252
CCGGCAG1800.019.52777716
CCGTATT1052.253546E-819.3809531
AGCTTCG1750.019.0285721
CGCCGGC1950.018.9743614
TCGCCGC1900.018.525
GTCCTAG801.6144018E-518.51
CCGTCCG609.229233E-418.528
Produced by FastQC (version 0.11.5)