FastQCFastQC Report
Fri 10 Feb 2017
ERR1633047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501972
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44260.881722486513192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32400.6454543281298558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16460.3279067358338712No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16200.3227271640649279No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16020.3191413066864287No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15780.31436016351509644No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12780.2545958738734431No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12540.24981473070211085No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10300.2051907277696764No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10250.20419465627564884No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9150.18228108340704263No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8710.17351565425960014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.17052743977751747No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8440.16813686819185134No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8110.16156279633126946No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7890.15718008175754822No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7810.15558636736710416No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7660.1525981528850215No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7620.15180129568979941No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7440.14821543831130024No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7410.1476177954148837No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7240.14423115233519002No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7230.1440319380363845No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7230.1440319380363845No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6970.1388523662674412No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6950.1384539376698302No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6670.13287593730327588No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6670.13287593730327588No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6650.13247750870566485No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6620.13187986580924832No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6370.12689950833911054No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6220.12391129385702788No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5930.11813407919166806No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5910.11773565059405704No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5890.11733722199644603No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5820.11594272190480744No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5780.11514586470958539No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5710.11375136461794681No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5500.10956786434303109No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5450.10857179284900352No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5380.10717729275736496No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5360.10677886415975393No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5220.10398986397647676No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5150.1025953638848382No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5130.10219693528722718No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5080.10120086379319963No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5040.10040400659797757No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5030.10020479229917206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.031.9785731
TGTGCTT250.005493430429.610
TGCGGCA250.005493430429.623
GTATCAA24650.027.0182571
ACTCGTC358.862406E-426.42857218
CCGCTCT1700.025.02941128
TTCGCCG1750.024.31428724
AATACTT551.8998913E-523.5454545
GCCGGCA1750.023.25714315
TGTCGAC400.001929679723.12500236
GCCGCTC1850.023.027
TGTGACG652.677849E-622.769238
TATATAC1155.456968E-1222.521744
TAGGACC1155.456968E-1222.521744
GCAGCTT1900.022.39473719
CCGTAAA502.6991646E-422.226
ATGAACA502.6991646E-422.24
AGCTCTA1250.022.26
CCGGCAG1850.022.016
CGCTCTC1950.021.8205129
Produced by FastQC (version 0.11.5)