##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501972
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73121409162264	33.0	33.0	33.0	27.0	33.0
2	31.255097893906434	33.0	33.0	33.0	27.0	33.0
3	31.677547751667422	33.0	33.0	33.0	27.0	33.0
4	31.811318161172338	33.0	33.0	33.0	27.0	33.0
5	31.924382236459405	33.0	33.0	33.0	33.0	33.0
6	34.77035770919493	37.0	37.0	37.0	27.0	37.0
7	34.908706063286395	37.0	37.0	37.0	27.0	37.0
8	35.120608320782836	37.0	37.0	37.0	33.0	37.0
9	35.177519861665594	37.0	37.0	37.0	33.0	37.0
10	35.19346895842796	37.0	37.0	37.0	33.0	37.0
11	35.23452503326879	37.0	37.0	37.0	33.0	37.0
12	35.133549281633236	37.0	37.0	37.0	33.0	37.0
13	35.20584215852677	37.0	37.0	37.0	33.0	37.0
14	35.150054186289275	37.0	37.0	37.0	33.0	37.0
15	35.2313037380571	37.0	37.0	37.0	33.0	37.0
16	35.22505637764656	37.0	37.0	37.0	33.0	37.0
17	35.19069788753158	37.0	37.0	37.0	33.0	37.0
18	35.14646633676779	37.0	37.0	37.0	33.0	37.0
19	35.182966380594934	37.0	37.0	37.0	33.0	37.0
20	35.11080100085264	37.0	37.0	37.0	33.0	37.0
21	35.168427721068106	37.0	37.0	37.0	33.0	37.0
22	35.16795558317994	37.0	37.0	37.0	33.0	37.0
23	35.14062537352681	37.0	37.0	37.0	33.0	37.0
24	35.14584877244149	37.0	37.0	37.0	33.0	37.0
25	35.14319922226738	37.0	37.0	37.0	33.0	37.0
26	35.108121568533704	37.0	37.0	37.0	33.0	37.0
27	35.04176926203055	37.0	37.0	37.0	27.0	37.0
28	35.02935621907198	37.0	37.0	37.0	27.0	37.0
29	35.077623851529566	37.0	37.0	37.0	33.0	37.0
30	35.03637254667591	37.0	37.0	37.0	27.0	37.0
31	35.01329954658826	37.0	37.0	37.0	27.0	37.0
32	35.04513996796634	37.0	37.0	37.0	27.0	37.0
33	35.010012510657965	37.0	37.0	37.0	27.0	37.0
34	34.966645549951	37.0	37.0	37.0	27.0	37.0
35	34.95767493007578	37.0	37.0	37.0	27.0	37.0
36	34.927772863825076	37.0	37.0	37.0	27.0	37.0
37	34.92676284733013	37.0	37.0	37.0	27.0	37.0
38	34.74757157769756	37.0	37.0	37.0	27.0	37.0
39	34.815354242866135	37.0	37.0	37.0	27.0	37.0
40	34.83634545353127	37.0	37.0	37.0	27.0	37.0
41	34.85172479739906	37.0	37.0	37.0	27.0	37.0
42	34.683398675623344	37.0	37.0	37.0	27.0	37.0
43	34.49030424007714	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	23.0
17	20.0
18	23.0
19	22.0
20	32.0
21	92.0
22	267.0
23	663.0
24	1396.0
25	2648.0
26	4290.0
27	6575.0
28	9255.0
29	12518.0
30	16398.0
31	20953.0
32	26167.0
33	34228.0
34	48356.0
35	86762.0
36	231275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.741682803024865	19.102659112460458	13.467484242148963	23.68817384236571
2	17.529862223390946	23.581793406803566	37.172392085614334	21.71595228419115
3	18.838700166543155	27.122628353772722	30.276987561059183	23.761683918624943
4	12.199086801654275	18.026503470313084	39.99306734240157	29.78134238563107
5	14.844453475492658	36.16297323356682	33.107225104189084	15.885348186751452
6	27.755332966779022	40.883953686659815	16.80990174750783	14.550811599053334
7	26.660849609141547	33.11200624736041	21.010136023523224	19.217008119974818
8	25.45420860127657	32.3488162686365	19.950714382475518	22.24626074761142
9	27.706923892169282	13.636417967536039	19.44231949192385	39.21433864837083
10	16.663080809288168	26.418804236092853	30.7620743786506	26.15604057596838
11	35.97292279250636	23.45469468416565	22.267775891882415	18.304606631445576
12	25.25200608798897	26.053046783485932	27.530619237726405	21.16432789079869
13	30.820643382499423	21.812571219111824	24.374865530348305	22.991919868040448
14	23.01502872670189	21.529487700509193	27.95474647988334	27.50073709290558
15	26.033922210800604	26.492115098053276	25.309977448941375	22.163985242204745
16	22.61859227207892	27.461292661742085	24.468695465085702	25.45141960109329
17	23.369231749978088	25.61915804068753	24.712135338226037	26.299474871108348
18	26.05942164104771	23.49194775804228	27.052305706294376	23.396324894615635
19	28.36074522084897	23.398516251902496	26.5652267457149	21.675511781533633
20	27.804738112882788	22.705051277760514	26.667622895300937	22.822587714055764
21	25.5277186775358	25.409186169746516	26.80707290446479	22.25602224825289
22	23.807702421649015	25.20917501374579	26.99931470281211	23.98380786179309
23	24.3059373829616	24.262508665821997	28.002358697297858	23.429195253918543
24	24.293785310734464	24.83186313180815	26.253456368084276	24.620895189373112
25	24.23222809240356	25.558596893850655	27.681623676220983	22.5275513375248
26	24.869913062880002	25.518554819790744	26.44131545185787	23.17021666547138
27	24.71592040990334	25.322129521168513	26.653677894384547	23.3082721745436
28	23.28177667280247	25.559991393942294	27.07302399337015	24.08520793988509
29	24.75556405536564	25.206585227861318	25.750639477899167	24.287211238873883
30	24.843815989736477	24.73863083996717	28.04977170041357	22.367781469882782
31	25.42392802785813	24.344385742631065	26.215008008414813	24.016678221095997
32	21.8803040807057	24.773692556556938	27.396149586032685	25.94985377670468
33	22.821193213964126	24.87310049166089	28.43306001131537	23.872646283059613
34	22.771788067860356	24.82787884583204	27.18358792920721	25.216745157100394
35	23.64693648251297	25.973161849664923	26.562437745531625	23.817463922290486
36	24.870709920075225	25.003585857378503	26.68256396771135	23.443140254834933
37	24.88764313547369	24.68225319340521	26.148470432613774	24.28163323850733
38	24.28681281027627	24.86015156223853	26.62817846413744	24.224857163347757
39	23.80929613603946	24.300757811192657	27.253312933789136	24.63663311897875
40	23.405090323763076	25.921764560573102	27.563688811328124	23.109456304335698
41	24.05253679488099	24.39060345995394	27.476034519853698	24.080825225311372
42	23.566254691496738	25.787892551775798	27.47424159116445	23.171611165563018
43	23.732200202401728	24.78205955710677	27.121233853681083	24.36450638681042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	20.0
2	38.0
3	197.0
4	356.0
5	356.0
6	457.0
7	558.0
8	560.0
9	562.0
10	734.5
11	907.0
12	907.0
13	1494.5
14	2082.0
15	3706.0
16	5330.0
17	5783.0
18	6236.0
19	6236.0
20	4940.5
21	3645.0
22	2994.0
23	2343.0
24	2998.0
25	3653.0
26	3653.0
27	4325.0
28	4997.0
29	5760.0
30	6523.0
31	8903.0
32	11283.0
33	11283.0
34	14218.0
35	17153.0
36	19737.5
37	22322.0
38	24612.0
39	26902.0
40	26902.0
41	29723.0
42	32544.0
43	35724.5
44	38905.0
45	39515.5
46	40126.0
47	40126.0
48	42762.5
49	45399.0
50	44696.0
51	43993.0
52	44107.0
53	44221.0
54	44221.0
55	40860.5
56	37500.0
57	33667.5
58	29835.0
59	26760.5
60	23686.0
61	23686.0
62	20108.0
63	16530.0
64	12835.5
65	9141.0
66	8208.5
67	7276.0
68	7276.0
69	6479.5
70	5683.0
71	5093.5
72	4504.0
73	3652.5
74	2801.0
75	2801.0
76	2134.0
77	1467.0
78	1105.5
79	744.0
80	665.5
81	587.0
82	587.0
83	589.0
84	591.0
85	678.5
86	766.0
87	601.0
88	436.0
89	436.0
90	353.5
91	271.0
92	165.5
93	60.0
94	36.5
95	13.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	501972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.904028973874524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.64331838252332	27.52202171529559
2	7.38333321227015	4.858828198806143
3	2.243581690918204	2.2146863088968094
4	1.1735295615066894	1.5445540277405754
5	0.7658735524195843	1.2600162779569106
6	0.5490526520528693	1.0839626622798155
7	0.4198394417218917	0.96700864083547
8	0.3107313145843682	0.8179449742539338
9	0.27298632579842275	0.8084114976188568
>10	2.4067752579532877	16.9955441579871
>50	0.44415414355328964	10.409030571116764
>100	0.35725441981460254	22.72151523610288
>500	0.023535341845894422	5.0834892819892845
>1k	0.006034703037408827	3.7129864491198634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4426	0.881722486513192	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3240	0.6454543281298558	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1646	0.3279067358338712	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1620	0.3227271640649279	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1602	0.3191413066864287	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1578	0.31436016351509644	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1278	0.2545958738734431	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1254	0.24981473070211085	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1030	0.2051907277696764	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1025	0.20419465627564884	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	915	0.18228108340704263	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	871	0.17351565425960014	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.17052743977751747	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	844	0.16813686819185134	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	811	0.16156279633126946	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	789	0.15718008175754822	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	781	0.15558636736710416	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	766	0.1525981528850215	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	762	0.15180129568979941	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	744	0.14821543831130024	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	741	0.1476177954148837	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	724	0.14423115233519002	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	723	0.1440319380363845	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	723	0.1440319380363845	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	697	0.1388523662674412	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	695	0.1384539376698302	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	667	0.13287593730327588	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	667	0.13287593730327588	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	665	0.13247750870566485	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	662	0.13187986580924832	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	637	0.12689950833911054	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	622	0.12391129385702788	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	593	0.11813407919166806	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	591	0.11773565059405704	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	589	0.11733722199644603	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	582	0.11594272190480744	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	578	0.11514586470958539	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	571	0.11375136461794681	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	550	0.10956786434303109	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	545	0.10857179284900352	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	538	0.10717729275736496	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	536	0.10677886415975393	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	522	0.10398986397647676	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	515	0.1025953638848382	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	513	0.10219693528722718	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	508	0.10120086379319963	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	504	0.10040400659797757	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	503	0.10020479229917206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0017929286892495995	0.0	0.0
2	0.0	0.0	0.0019921429880551105	0.0	0.0
3	0.0	0.0	0.002390571585666133	0.0	0.0
4	0.0	0.0	0.0029882144820826658	0.0	0.0
5	0.0	0.0	0.004581928872526755	0.0	0.0
6	0.0	0.0	0.006574071860581865	1.9921429880551106E-4	0.0
7	0.0	0.0	0.01673400109966293	3.984285976110221E-4	0.0
8	0.0	0.0	0.019124572685329064	3.984285976110221E-4	0.0
9	0.0	0.0	0.023706501557855815	7.968571952220443E-4	0.0
10	7.968571952220443E-4	0.0	0.025499430247105416	7.968571952220443E-4	0.0
11	7.968571952220443E-4	0.0	0.03864757396826915	7.968571952220443E-4	0.0
12	7.968571952220443E-4	0.0	0.0432295028407959	7.968571952220443E-4	0.0
13	7.968571952220443E-4	0.0	0.04601850302407306	7.968571952220443E-4	0.0
14	7.968571952220443E-4	0.0	0.049803574701377765	9.960714940275553E-4	0.0
15	7.968571952220443E-4	0.0	0.05518236076912657	0.0013945000916385775	0.0
16	7.968571952220443E-4	0.0	0.057572932354792695	0.0013945000916385775	0.0
17	7.968571952220443E-4	0.0	0.059365861044042297	0.0015937143904440885	0.0
18	7.968571952220443E-4	0.0	0.060760361135680875	0.0017929286892495995	0.0
19	7.968571952220443E-4	0.0	0.06175643262970843	0.0017929286892495995	0.0
20	7.968571952220443E-4	0.0	0.06374857561776354	0.0019921429880551105	0.0
21	7.968571952220443E-4	0.0	0.06514307570940212	0.0025897858844716438	0.0
22	7.968571952220443E-4	0.0	0.06613914720342967	0.002789000183277155	0.0
23	7.968571952220443E-4	0.0	0.06633836150223518	0.003585857378499199	0.0
24	7.968571952220443E-4	0.0	0.0667367900998462	0.0043827145737212435	0.0
25	7.968571952220443E-4	0.0	0.0667367900998462	0.0043827145737212435	0.0
26	7.968571952220443E-4	0.0	0.0667367900998462	0.004980357470137776	0.0
27	7.968571952220443E-4	0.0	0.06693600439865172	0.0059764289641653316	0.0
28	7.968571952220443E-4	0.0	0.06733443299626274	0.02051907277696764	0.0
29	7.968571952220443E-4	0.0	0.06733443299626274	0.04641693162168408	0.0
30	7.968571952220443E-4	0.0	0.06733443299626274	0.08247471970548158	0.0
31	7.968571952220443E-4	0.0	0.06733443299626274	0.17730072593690485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	700	0.0	31.978573	1
TGTGCTT	25	0.0054934304	29.6	10
TGCGGCA	25	0.0054934304	29.6	23
GTATCAA	2465	0.0	27.018257	1
ACTCGTC	35	8.862406E-4	26.428572	18
CCGCTCT	170	0.0	25.029411	28
TTCGCCG	175	0.0	24.314287	24
AATACTT	55	1.8998913E-5	23.545454	5
GCCGGCA	175	0.0	23.257143	15
TGTCGAC	40	0.0019296797	23.125002	36
GCCGCTC	185	0.0	23.0	27
TGTGACG	65	2.677849E-6	22.76923	8
TATATAC	115	5.456968E-12	22.52174	4
TAGGACC	115	5.456968E-12	22.52174	4
GCAGCTT	190	0.0	22.394737	19
CCGTAAA	50	2.6991646E-4	22.2	26
ATGAACA	50	2.6991646E-4	22.2	4
AGCTCTA	125	0.0	22.2	6
CCGGCAG	185	0.0	22.0	16
CGCTCTC	195	0.0	21.82051	29
>>END_MODULE