FastQCFastQC Report
Fri 10 Feb 2017
ERR1633046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397762
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11280.2835866674041261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.19232606432992594No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4180.10508796717635167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3980.1000598347755693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC303.595285E-430.83333225
GACATCG250.005492075329.622
GCCGCTC453.9995994E-628.77777727
CGCTCTC509.071582E-625.90000229
GGTATCA3350.025.4029851
TCACTAG400.001928979623.12516
GCCGGCA652.6755279E-622.7692315
TATTGTC652.6755279E-622.769235
CCGCTCT603.7184058E-521.58333228
AGCTTCG705.0880917E-621.14285921
GCTTCGC705.0880917E-621.14285922
GTATCAA6950.020.4964031
TTCGGTC555.1349704E-420.18181828
CGAACTA1052.2482709E-819.3809529
GCGAACT1052.2482709E-819.3809528
TGCTCGC801.6123433E-518.510
ACAGACG500.007026992318.58
ACCCGCA500.007026992318.526
ACATCGT500.007026992318.523
TTCGCCG801.6123433E-518.524
Produced by FastQC (version 0.11.5)