##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397762
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74890763823593	33.0	33.0	33.0	27.0	33.0
2	31.26586752882377	33.0	33.0	33.0	27.0	33.0
3	31.682883231681256	33.0	33.0	33.0	27.0	33.0
4	31.810715955772547	33.0	33.0	33.0	27.0	33.0
5	31.928610576173693	33.0	33.0	33.0	33.0	33.0
6	34.82340444788592	37.0	37.0	37.0	27.0	37.0
7	34.95533509988385	37.0	37.0	37.0	27.0	37.0
8	35.15915296081577	37.0	37.0	37.0	33.0	37.0
9	35.19738939365752	37.0	37.0	37.0	33.0	37.0
10	35.248688914476496	37.0	37.0	37.0	33.0	37.0
11	35.30883794832086	37.0	37.0	37.0	33.0	37.0
12	35.222416922682406	37.0	37.0	37.0	33.0	37.0
13	35.26326295624016	37.0	37.0	37.0	33.0	37.0
14	35.20356394024567	37.0	37.0	37.0	33.0	37.0
15	35.27427205213168	37.0	37.0	37.0	33.0	37.0
16	35.220926081425574	37.0	37.0	37.0	33.0	37.0
17	35.24497563869852	37.0	37.0	37.0	33.0	37.0
18	35.225810912052935	37.0	37.0	37.0	33.0	37.0
19	35.260942473137206	37.0	37.0	37.0	33.0	37.0
20	35.14694214128046	37.0	37.0	37.0	33.0	37.0
21	35.22866940532278	37.0	37.0	37.0	33.0	37.0
22	35.24683856175301	37.0	37.0	37.0	33.0	37.0
23	35.25604255811264	37.0	37.0	37.0	33.0	37.0
24	35.24242888963752	37.0	37.0	37.0	33.0	37.0
25	35.218862033075055	37.0	37.0	37.0	33.0	37.0
26	35.14751786244035	37.0	37.0	37.0	33.0	37.0
27	35.08029424630809	37.0	37.0	37.0	33.0	37.0
28	35.11499338800589	37.0	37.0	37.0	33.0	37.0
29	35.16138545160171	37.0	37.0	37.0	33.0	37.0
30	35.1330142145303	37.0	37.0	37.0	33.0	37.0
31	35.116479201130325	37.0	37.0	37.0	33.0	37.0
32	35.15094956280389	37.0	37.0	37.0	33.0	37.0
33	35.11982542324304	37.0	37.0	37.0	33.0	37.0
34	35.09038822210267	37.0	37.0	37.0	33.0	37.0
35	35.06632860856492	37.0	37.0	37.0	33.0	37.0
36	35.073888405629496	37.0	37.0	37.0	33.0	37.0
37	35.07666896284713	37.0	37.0	37.0	33.0	37.0
38	34.93113721270508	37.0	37.0	37.0	27.0	37.0
39	34.99991452174919	37.0	37.0	37.0	27.0	37.0
40	35.03933759383752	37.0	37.0	37.0	27.0	37.0
41	35.07881346131607	37.0	37.0	37.0	33.0	37.0
42	34.95344955023356	37.0	37.0	37.0	27.0	37.0
43	34.769915175406396	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	16.0
17	20.0
18	16.0
19	11.0
20	30.0
21	64.0
22	178.0
23	504.0
24	1041.0
25	1913.0
26	3165.0
27	4767.0
28	6875.0
29	9242.0
30	12186.0
31	15590.0
32	20289.0
33	26264.0
34	37279.0
35	68692.0
36	189611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80681412502954	20.545200396216835	13.748171016839214	24.89981446191441
2	15.783056199435844	23.07083130112982	38.38501415419271	22.761098345241628
3	17.66986288282943	26.84997561355786	30.876001226864307	24.604160276748406
4	12.980123792619708	17.967779727575785	38.98386472312589	30.068231756678614
5	13.593807352135196	37.32860353678833	35.77843031762713	13.299158793449351
6	29.39747889441425	39.576178719938056	17.742770802640777	13.283571583006923
7	26.13547799940668	32.54006164490323	23.829576480407884	17.494883875282206
8	23.883126090476214	36.74131767237695	20.895409817931327	18.480146419215508
9	25.615066295925704	16.16946817443597	20.211080998184844	38.00438453145348
10	14.610998536813472	28.579150346186914	34.39871078685244	22.411140330147173
11	33.2339439162112	23.988716870892645	24.414096871998833	18.36324234089732
12	22.29473906506906	25.970303850040978	30.540876202352162	21.1940808825378
13	27.521231289062303	22.385496854903185	26.850981240038013	23.2422906159965
14	21.950814808855547	21.30796808141552	27.563216194608835	29.178000915120094
15	23.495205675755855	29.005033160533184	24.60064058406786	22.899120579643103
16	23.0424223530654	28.839104791307363	24.92344668419809	23.195026171429145
17	22.34602601555704	28.083376491469775	26.513090742705437	23.05750675026775
18	22.553436477089313	27.82744455226995	27.6079665729758	22.011152397664937
19	24.32082501596432	26.70139430111474	27.62179393707795	21.35598674584299
20	24.121710972893336	26.443702515574643	27.748502873577667	21.686083637954354
21	22.71257686757408	27.522739728782536	26.952046701293735	22.812636702349646
22	22.407369230846584	27.29471392440706	27.204207541192975	23.093709303553382
23	22.365887138540135	27.523493948642656	27.67936605306691	22.431252859750302
24	23.14826454010187	27.603944067055174	26.630749040883746	22.617042351959213
25	23.189495225788285	26.927660259149945	27.653219764582843	22.22962475047893
26	23.101754315394633	27.229096796576847	27.182837978489648	22.48631090953887
27	22.949653310270964	27.269070449163067	26.68982959659294	23.09144664397303
28	22.43980068483163	27.378180922260043	27.53053333400375	22.651485058904573
29	23.383329729838444	27.17780984608887	27.119734916859834	22.319125507212856
30	22.83777736435356	27.55617680924774	27.132305247861787	22.473740578536912
31	23.2146358877922	26.961851559475264	27.24619244673951	22.57732010599303
32	22.332701464694967	27.049341063248878	27.43022209260814	23.187735379448014
33	22.482288403618245	27.01087585038289	27.731155816794967	22.775679929203896
34	22.681151040069185	26.874864868941728	27.554416962907467	22.889567128081616
35	22.85688426747653	27.49609062705839	27.16347966874664	22.483545436718437
36	23.06831723492943	27.29395970454694	26.884921133743294	22.752801926780336
37	23.281258642102564	26.551304548951382	27.06668812003158	23.10074868891448
38	23.352658122193674	27.01087585038289	26.929168698870175	22.707297328553256
39	22.871968664678878	27.095599881336074	27.34524665503492	22.687184798950124
40	22.80836278980898	26.978192989777806	27.621039717217833	22.59240450319538
41	22.57405181993252	26.619687149602022	27.64894585204218	23.15731517842328
42	22.766629290882488	26.961600152855226	27.318848959930815	22.952921596331475
43	22.969263026634017	26.412025281449715	27.50765533158019	23.11105636033608
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	73.0
4	130.0
5	130.0
6	143.5
7	157.0
8	148.0
9	139.0
10	196.0
11	253.0
12	253.0
13	401.5
14	550.0
15	1080.0
16	1610.0
17	1902.5
18	2195.0
19	2195.0
20	2233.5
21	2272.0
22	2855.5
23	3439.0
24	4481.5
25	5524.0
26	5524.0
27	7142.0
28	8760.0
29	11052.0
30	13344.0
31	15129.0
32	16914.0
33	16914.0
34	19136.0
35	21358.0
36	23330.0
37	25302.0
38	27519.0
39	29736.0
40	29736.0
41	31211.5
42	32687.0
43	31895.0
44	31103.0
45	30880.0
46	30657.0
47	30657.0
48	29998.0
49	29339.0
50	29496.5
51	29654.0
52	29408.0
53	29162.0
54	29162.0
55	26009.0
56	22856.0
57	20128.0
58	17400.0
59	15236.5
60	13073.0
61	13073.0
62	11541.0
63	10009.0
64	8351.0
65	6693.0
66	5814.0
67	4935.0
68	4935.0
69	4064.5
70	3194.0
71	2697.5
72	2201.0
73	1779.0
74	1357.0
75	1357.0
76	1052.0
77	747.0
78	581.5
79	416.0
80	320.5
81	225.0
82	225.0
83	191.0
84	157.0
85	129.5
86	102.0
87	77.0
88	52.0
89	52.0
90	42.0
91	32.0
92	18.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	397762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.46075463613144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33870904862682	55.17702430758885
2	6.117806118445122	7.642455737512489
3	1.783593892508623	3.342138614714511
4	0.9024991229714295	2.2548310511696914
5	0.5411143301100906	1.6899204701550503
6	0.3648461947105843	1.367314118864643
7	0.262046437452923	1.1457332763113572
8	0.19914235163360836	0.9950865250439218
9	0.1455926298253299	0.818444297551414
>10	1.1727938219285519	14.820894093252068
>50	0.12564947182576522	5.470674431358532
>100	0.045395938207463224	4.796238932904609
>500	4.053208768523502E-4	0.19367235596051866
>1k	4.053208768523502E-4	0.2855717876123726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	0.2835866674041261	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.19232606432992594	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	418	0.10508796717635167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	398	0.1000598347755693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	5.028132400782377E-4	0.0	0.0
4	0.0	0.0	5.028132400782377E-4	0.0	0.0
5	0.0	0.0	7.542198601173567E-4	0.0	0.0
6	0.0	0.0	0.002011252960312951	0.0	0.0
7	0.0	0.0	0.003771099300586783	0.0	0.0
8	0.0	0.0	0.004022505920625902	0.0	0.0
9	0.0	0.0	0.00452531916070414	0.0	0.0
10	0.0	0.0	0.005028132400782377	0.0	0.0
11	0.0	0.0	0.0062851655009779715	0.0	0.0
12	0.0	0.0	0.0067879787410562095	2.5140662003911887E-4	0.0
13	0.0	0.0	0.0070393853610953286	2.5140662003911887E-4	0.0
14	0.0	0.0	0.007542198601173566	2.5140662003911887E-4	0.0
15	0.0	0.0	0.00879923170136916	2.5140662003911887E-4	0.0
16	0.0	0.0	0.00879923170136916	2.5140662003911887E-4	0.0
17	0.0	0.0	0.00879923170136916	2.5140662003911887E-4	0.0
18	0.0	0.0	0.00879923170136916	2.5140662003911887E-4	0.0
19	0.0	2.5140662003911887E-4	0.00905063832140828	2.5140662003911887E-4	0.0
20	0.0	2.5140662003911887E-4	0.009302044941447398	5.028132400782377E-4	0.0
21	0.0	2.5140662003911887E-4	0.009302044941447398	5.028132400782377E-4	0.0
22	0.0	2.5140662003911887E-4	0.009553451561486516	7.542198601173567E-4	0.0
23	0.0	2.5140662003911887E-4	0.010056264801564754	0.0012570331001955943	0.0
24	0.0	2.5140662003911887E-4	0.010056264801564754	0.002011252960312951	0.0
25	0.0	2.5140662003911887E-4	0.010056264801564754	0.002011252960312951	0.0
26	0.0	2.5140662003911887E-4	0.010056264801564754	0.00226265958035207	0.0
27	0.0	2.5140662003911887E-4	0.010056264801564754	0.0032682860605085452	0.0
28	0.0	2.5140662003911887E-4	0.010056264801564754	0.00879923170136916	0.0
29	0.0	2.5140662003911887E-4	0.010056264801564754	0.017095650162660084	0.0
30	0.0	2.5140662003911887E-4	0.010056264801564754	0.03519692680547664	0.0
31	0.0	2.5140662003911887E-4	0.010056264801564754	0.0892493501138872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	30	3.595285E-4	30.833332	25
GACATCG	25	0.0054920753	29.6	22
GCCGCTC	45	3.9995994E-6	28.777777	27
CGCTCTC	50	9.071582E-6	25.900002	29
GGTATCA	335	0.0	25.402985	1
TCACTAG	40	0.0019289796	23.125	16
GCCGGCA	65	2.6755279E-6	22.76923	15
TATTGTC	65	2.6755279E-6	22.76923	5
CCGCTCT	60	3.7184058E-5	21.583332	28
AGCTTCG	70	5.0880917E-6	21.142859	21
GCTTCGC	70	5.0880917E-6	21.142859	22
GTATCAA	695	0.0	20.496403	1
TTCGGTC	55	5.1349704E-4	20.181818	28
CGAACTA	105	2.2482709E-8	19.38095	29
GCGAACT	105	2.2482709E-8	19.38095	28
TGCTCGC	80	1.6123433E-5	18.5	10
ACAGACG	50	0.0070269923	18.5	8
ACCCGCA	50	0.0070269923	18.5	26
ACATCGT	50	0.0070269923	18.5	23
TTCGCCG	80	1.6123433E-5	18.5	24
>>END_MODULE