FastQCFastQC Report
Fri 10 Feb 2017
ERR1633045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357243
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10650.2981164081591522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.22925571669703818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3680.10301111568316246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC200.001840238537.035
CCGTCCG405.928848E-527.7528
CGTTAAA509.068204E-625.89999817
TCCGCCG451.3207196E-424.66666631
CGAGTCG451.3207196E-424.66666621
GTATCAA4800.022.3541681
TCGCCGT502.6970534E-422.19999925
GAGTCGC502.6970534E-422.19999922
GGTATCA1950.021.8205131
GTATCTA450.003820725520.5555551
AACTATG450.003820725520.5555557
AACCGTG555.133562E-420.1818188
CGTCCGC555.133562E-420.18181829
CCTTAGG656.885121E-519.9230771
GGTACCG759.236486E-619.7333346
TATATAC759.236486E-619.7333343
AGGGTAC902.143779E-618.54
CGCCGTC609.219835E-418.526
GCCGTTA701.2162147E-418.515
AGTCGCC609.219835E-418.523
Produced by FastQC (version 0.11.5)