##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357243
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.696909386607995	33.0	33.0	33.0	27.0	33.0
2	31.185089700847882	33.0	33.0	33.0	27.0	33.0
3	31.62704657614005	33.0	33.0	33.0	27.0	33.0
4	31.7784757154094	33.0	33.0	33.0	27.0	33.0
5	31.873811383288125	33.0	33.0	33.0	33.0	33.0
6	34.770092066184645	37.0	37.0	37.0	27.0	37.0
7	34.95393051788279	37.0	37.0	37.0	27.0	37.0
8	35.1301830966597	37.0	37.0	37.0	33.0	37.0
9	35.212158110865715	37.0	37.0	37.0	33.0	37.0
10	35.24171782232262	37.0	37.0	37.0	33.0	37.0
11	35.270818462503115	37.0	37.0	37.0	33.0	37.0
12	35.250888050990504	37.0	37.0	37.0	33.0	37.0
13	35.26949163454567	37.0	37.0	37.0	33.0	37.0
14	35.24670042520077	37.0	37.0	37.0	33.0	37.0
15	35.27496130085124	37.0	37.0	37.0	33.0	37.0
16	35.2541379397217	37.0	37.0	37.0	33.0	37.0
17	35.21661446130505	37.0	37.0	37.0	33.0	37.0
18	35.22297147879735	37.0	37.0	37.0	33.0	37.0
19	35.236732420229366	37.0	37.0	37.0	33.0	37.0
20	35.246347724098165	37.0	37.0	37.0	33.0	37.0
21	35.236357325405955	37.0	37.0	37.0	33.0	37.0
22	35.23873105981083	37.0	37.0	37.0	33.0	37.0
23	35.22388682213507	37.0	37.0	37.0	33.0	37.0
24	35.23385762632158	37.0	37.0	37.0	33.0	37.0
25	35.22065932712467	37.0	37.0	37.0	33.0	37.0
26	35.17949966829301	37.0	37.0	37.0	33.0	37.0
27	35.12219133754895	37.0	37.0	37.0	33.0	37.0
28	35.08048023334257	37.0	37.0	37.0	33.0	37.0
29	35.125908695201865	37.0	37.0	37.0	33.0	37.0
30	35.09426356849539	37.0	37.0	37.0	33.0	37.0
31	35.08646775444166	37.0	37.0	37.0	33.0	37.0
32	35.10133718505331	37.0	37.0	37.0	33.0	37.0
33	35.088779906114326	37.0	37.0	37.0	33.0	37.0
34	35.02705161472723	37.0	37.0	37.0	27.0	37.0
35	35.018332059690465	37.0	37.0	37.0	27.0	37.0
36	35.01656015653211	37.0	37.0	37.0	27.0	37.0
37	35.0260606925818	37.0	37.0	37.0	27.0	37.0
38	34.878558852097875	37.0	37.0	37.0	27.0	37.0
39	34.94627466458405	37.0	37.0	37.0	27.0	37.0
40	34.98878354509396	37.0	37.0	37.0	27.0	37.0
41	35.00634022220169	37.0	37.0	37.0	27.0	37.0
42	34.91990045991104	37.0	37.0	37.0	27.0	37.0
43	34.716394722919695	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	15.0
17	14.0
18	10.0
19	12.0
20	24.0
21	69.0
22	186.0
23	460.0
24	1024.0
25	1809.0
26	2981.0
27	4566.0
28	6238.0
29	8495.0
30	11033.0
31	14261.0
32	17919.0
33	23464.0
34	33766.0
35	60457.0
36	170431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.45033772530183	20.5081695092696	13.762900882592522	25.278591882836054
2	15.999193826051176	22.91633425987353	37.74069750841864	23.343774405656653
3	17.910497896389852	26.330816838958356	30.115075732764534	25.643609531887257
4	13.083531377801663	17.802448193526534	38.578222666364354	30.53579776230745
5	14.20489694689609	36.70918674403697	35.08340261390706	14.002513695159877
6	30.269872327799284	39.1663377588924	17.354573777512787	13.209216135795524
7	26.670361630598784	31.92252892289002	23.60158211637457	17.80552733013663
8	24.6840385955778	35.91616910618263	20.748622086366982	18.651170211872593
9	25.89973771354512	15.479099660455208	20.08436834311659	38.53679428288308
10	14.88958496037711	28.09852117466263	34.02529930607457	22.986594558885688
11	34.47457332963837	23.3127031180457	24.093124287949657	18.119599264366272
12	22.85503144918165	25.19657488040353	30.65420456104108	21.294189109373733
13	28.228124833796603	21.938008582393497	26.093443398471067	23.74042318533883
14	22.02254487841609	20.794249292498385	27.433987509902224	29.749218319183303
15	23.83195751911164	28.6743197207503	23.815162228511124	23.678560531626932
16	23.311303510495655	28.024062053000335	25.0521353812391	23.612499055264905
17	22.62241667436451	27.470937149223356	26.499329587983528	23.407316588428603
18	23.430550073759317	27.2377625313862	27.16414317425394	22.167544220600544
19	24.424271434289828	26.511926055933916	27.02194304716957	22.041859462606688
20	24.162824743941798	26.468258300372575	26.96287960855776	22.406037347127867
21	23.5016501373015	26.695554566499553	27.045456454010296	22.757338842188652
22	23.156506915460906	26.618016308227173	27.060012372530746	23.16546440378118
23	23.164904560761162	26.387920827000112	27.304383850768243	23.142790761470483
24	23.222568391822936	26.71179001408005	26.657765162648396	23.40787643144862
25	23.517885584882	26.42599015236128	27.22628574947585	22.829838513280876
26	23.365048440417308	26.80388419087288	26.832156263383748	22.998911105326066
27	23.21389082501267	26.86378739401472	26.689116371769355	23.233205409203258
28	23.022984355186807	26.64908759583813	27.146788040633407	23.18114000834166
29	23.69843495883754	26.61997575879723	26.820399559963388	22.86118972240184
30	23.491293041431184	26.654406104528288	26.748459731891177	23.105841122149346
31	23.474217829320658	26.490372099663258	26.86378739401472	23.17162267700137
32	22.933689393494063	26.384841690390015	27.26127593822692	23.420192977889002
33	22.951604370134614	26.483933904933053	27.38080242300059	23.18365930193174
34	23.341814955086594	26.309262882687694	27.030340692469835	23.31858146975588
35	23.361689382297204	26.667562415498697	26.854549984184434	23.11619821801967
36	23.628454581335394	26.68211833401914	26.631452540707585	23.057974543937878
37	23.363368911357256	26.221647450055002	26.89149962350557	23.523484015082172
38	23.434748896409445	26.655245869058312	26.760496356821545	23.14950887771069
39	23.005349300056263	26.443905129001827	27.126633691912787	23.42411187902912
40	23.032221765017088	26.54915561676506	27.294866519427952	23.1237560987899
41	23.009548122706395	26.254958109746028	27.214249124545475	23.521244643002102
42	22.93284962896404	26.827117676203592	27.074848212561196	23.16518448227117
43	23.448465050399868	25.97783581483752	27.24867947027653	23.325019664486078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	10.5
2	17.0
3	62.5
4	108.0
5	108.0
6	142.0
7	176.0
8	157.5
9	139.0
10	197.0
11	255.0
12	255.0
13	410.5
14	566.0
15	1027.5
16	1489.0
17	1728.0
18	1967.0
19	1967.0
20	1924.0
21	1881.0
22	2283.5
23	2686.0
24	3627.0
25	4568.0
26	4568.0
27	5848.5
28	7129.0
29	8858.5
30	10588.0
31	12461.0
32	14334.0
33	14334.0
34	16375.0
35	18416.0
36	20286.0
37	22156.0
38	23609.0
39	25062.0
40	25062.0
41	26051.0
42	27040.0
43	27307.5
44	27575.0
45	27608.0
46	27641.0
47	27641.0
48	27093.0
49	26545.0
50	26009.5
51	25474.0
52	24394.0
53	23314.0
54	23314.0
55	22265.0
56	21216.0
57	19311.5
58	17407.0
59	16089.5
60	14772.0
61	14772.0
62	13149.0
63	11526.0
64	9663.0
65	7800.0
66	6714.0
67	5628.0
68	5628.0
69	4697.0
70	3766.0
71	3156.0
72	2546.0
73	2005.5
74	1465.0
75	1465.0
76	1174.5
77	884.0
78	677.0
79	470.0
80	387.5
81	305.0
82	305.0
83	232.0
84	159.0
85	127.5
86	96.0
87	72.5
88	49.0
89	49.0
90	34.0
91	19.0
92	11.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	357243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.64927500417741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.67821685666358	68.73770305730666
2	6.443328804348003	9.877529629336156
3	1.6217577783833887	3.729196738364165
4	0.703244563422413	2.156127437478289
5	0.3750711891869161	1.4374467363065893
6	0.22196602658688053	1.0208121008065416
7	0.17300865458698073	0.9282691560478158
8	0.1198799339709931	0.7350968021140224
9	0.09191755977681931	0.6340872885341704
>10	0.5257355059299951	7.255999542957989
>50	0.03376262157926472	1.7620090478637485
>100	0.011376535530593238	1.1957695500765273
>500	3.669850171159109E-4	0.2303776197395144
>1k	3.669850171159109E-4	0.29957529306786673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.2981164081591522	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	819	0.22925571669703818	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.10301111568316246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	2.799215100085936E-4	0.0	0.0
7	0.0	0.0	0.001959450570060155	0.0	0.0
8	0.0	0.0	0.002519293590077342	0.0	0.0
9	2.799215100085936E-4	0.0	0.003638979630111717	2.799215100085936E-4	0.0
10	5.598430200171872E-4	0.0	0.00391890114012031	2.799215100085936E-4	0.0
11	5.598430200171872E-4	0.0	0.004758665670146091	2.799215100085936E-4	0.0
12	5.598430200171872E-4	0.0	0.005038587180154684	2.799215100085936E-4	0.0
13	5.598430200171872E-4	0.0	0.006158273220189059	2.799215100085936E-4	0.0
14	5.598430200171872E-4	0.0	0.006158273220189059	2.799215100085936E-4	0.0
15	5.598430200171872E-4	0.0	0.006998037750214839	2.799215100085936E-4	0.0
16	5.598430200171872E-4	0.0	0.00783780228024062	2.799215100085936E-4	0.0
17	5.598430200171872E-4	0.0	0.00783780228024062	2.799215100085936E-4	0.0
18	5.598430200171872E-4	0.0	0.008397645300257807	2.799215100085936E-4	0.0
19	5.598430200171872E-4	0.0	0.008397645300257807	2.799215100085936E-4	0.0
20	5.598430200171872E-4	0.0	0.008677566810266402	2.799215100085936E-4	0.0
21	5.598430200171872E-4	0.0	0.009237409830283589	5.598430200171872E-4	0.0
22	5.598430200171872E-4	0.0	0.009237409830283589	5.598430200171872E-4	0.0
23	5.598430200171872E-4	0.0	0.009237409830283589	8.397645300257808E-4	0.0
24	5.598430200171872E-4	0.0	0.009237409830283589	8.397645300257808E-4	0.0
25	5.598430200171872E-4	0.0	0.009237409830283589	0.001399607550042968	0.0
26	5.598430200171872E-4	0.0	0.009237409830283589	0.0016795290600515615	0.0
27	5.598430200171872E-4	0.0	0.009237409830283589	0.002239372080068749	0.0
28	5.598430200171872E-4	0.0	0.009237409830283589	0.0053185086901632785	0.0
29	8.397645300257808E-4	0.0	0.009237409830283589	0.01791497664054999	0.0
30	8.397645300257808E-4	0.0	0.009237409830283589	0.03387050271103982	0.0
31	8.397645300257808E-4	0.0	0.009237409830283589	0.08509613904261246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGTC	20	0.0018402385	37.0	35
CCGTCCG	40	5.928848E-5	27.75	28
CGTTAAA	50	9.068204E-6	25.899998	17
TCCGCCG	45	1.3207196E-4	24.666666	31
CGAGTCG	45	1.3207196E-4	24.666666	21
GTATCAA	480	0.0	22.354168	1
TCGCCGT	50	2.6970534E-4	22.199999	25
GAGTCGC	50	2.6970534E-4	22.199999	22
GGTATCA	195	0.0	21.820513	1
GTATCTA	45	0.0038207255	20.555555	1
AACTATG	45	0.0038207255	20.555555	7
AACCGTG	55	5.133562E-4	20.181818	8
CGTCCGC	55	5.133562E-4	20.181818	29
CCTTAGG	65	6.885121E-5	19.923077	1
GGTACCG	75	9.236486E-6	19.733334	6
TATATAC	75	9.236486E-6	19.733334	3
AGGGTAC	90	2.143779E-6	18.5	4
CGCCGTC	60	9.219835E-4	18.5	26
GCCGTTA	70	1.2162147E-4	18.5	15
AGTCGCC	60	9.219835E-4	18.5	23
>>END_MODULE