FastQCFastQC Report
Fri 10 Feb 2017
ERR1633044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374535
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52951.4137530537866954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40591.083743842364532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21530.5748461425500955No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10890.29076054307341104No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10270.274206682953529No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10000.2669977438690643No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9990.2667307461251952No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9580.2557838386265636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.21146221314429894No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7530.20104930113340544No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7500.20024830790179826No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7300.19490835302441695No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7190.19197137784185725No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6680.17835449290453495No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6620.17675250644132057No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6070.16206763052852205No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6060.16180063278465295No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5950.15886365760209326No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5930.15832966211435515No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5890.15726167113887887No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.15699467339500983No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5790.15459169370018824No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5760.15379070046858104No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5730.15298970723697386No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5690.15192171626149759No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5600.14951873656667602No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5570.14871774333506882No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5510.14711575687185444No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5370.14337778845768753No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5330.14230979748221126No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5170.13803783358030625No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5010.13376586967840123No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4970.13269787870292496No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4880.13029489900810337No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4860.12976090352036526No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4850.1294939057764962No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4840.12922690803262712No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4820.12869291254488902No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4810.12842591480101995No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4810.12842591480101995No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4800.12815891705715085No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4730.12628993285006743No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4720.12602293510619836No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4690.12522194187459115No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4550.12148397346042425No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4540.1212169757165552No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4470.11934799150947173No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4400.11747900730238829No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4330.11561002309530485No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4300.11480902986369765No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4280.11427503437595954No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4270.11400803663209046No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4250.11347404114435233No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4240.11320704340048326No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4240.11320704340048326No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4200.112139052425007No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4180.11160505693726887No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4160.11107106144953074No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4100.10946907498631638No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4090.1092020772424473No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4060.10840108401084012No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4050.10813408626697105No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4040.10786708852310199No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC4030.10760009077923291No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4000.10679909754762573No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA3900.10412912010893509No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3890.103862122365066No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT3830.10226013590185162No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3800.10145914267024445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT200.00184035237.016
TTGTATC200.00184035237.017
TATCGTT200.00184035237.013
ACTAGTG303.5948862E-430.8333348
GGTATCA8550.029.859651
GGGTGGC250.00549167129.634
CACGCTG250.00549167129.614
ACCGAAG250.00549167129.637
GAATCAA250.00549167129.61
ATCGTTA250.00549167129.614
CTAGTGG250.00549167129.69
CACAGAT250.00549167129.637
GCCGCTC2250.027.13333327
AGATATA856.91216E-1126.1176471
CCGCTCT2350.025.97872228
CGCTCTC2350.025.97872229
GTTATCG509.069734E-625.90000211
CGCCGGC2450.025.67346814
TTCGCCG2500.025.1624
CGGCAGC2450.024.91836717
Produced by FastQC (version 0.11.5)