FastQCFastQC Report
Fri 10 Feb 2017
ERR1633043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345290
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8470.24530105129021978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.17202930869703725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1150.025.739131
GTATCAA3800.023.8552631
AGGTCGC555.133084E-420.18181835
GGTCGCC609.218984E-418.536
ACGGAAC801.6113383E-518.534
TAGGGGT500.007025158518.54
TAAGGGA701.21606456E-418.54
TACCGCA500.007025158518.57
GTATTAG1107.768758E-716.8181821
GTATAAA803.373999E-416.18751
GCTCGGA803.373999E-416.187511
CTAAGAC1151.2359724E-616.0869563
GTCGCCC700.002587570315.85714337
AAGACTC700.002587570315.8571435
CCGAGGT700.002587570315.85714332
TTCATAC855.3506636E-415.2352943
ATTAGAG750.004096677514.83
GAGGTCG750.004096677514.834
TCTAAGC1001.0898482E-414.83
AGTGTGA750.004096677514.88
Produced by FastQC (version 0.11.5)