##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345290
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75772539025167	33.0	33.0	33.0	27.0	33.0
2	31.282588548756117	33.0	33.0	33.0	27.0	33.0
3	31.69702568855165	33.0	33.0	33.0	27.0	33.0
4	31.82279533146051	33.0	33.0	33.0	27.0	33.0
5	31.923365866373192	33.0	33.0	33.0	33.0	33.0
6	34.828497784471026	37.0	37.0	37.0	27.0	37.0
7	34.97538011526543	37.0	37.0	37.0	27.0	37.0
8	35.15246604303629	37.0	37.0	37.0	33.0	37.0
9	35.20600075299024	37.0	37.0	37.0	33.0	37.0
10	35.27299661154392	37.0	37.0	37.0	33.0	37.0
11	35.30684352283588	37.0	37.0	37.0	33.0	37.0
12	35.22514697790263	37.0	37.0	37.0	33.0	37.0
13	35.266546960525936	37.0	37.0	37.0	33.0	37.0
14	35.23805496828753	37.0	37.0	37.0	33.0	37.0
15	35.29383996061282	37.0	37.0	37.0	33.0	37.0
16	35.28355295548669	37.0	37.0	37.0	33.0	37.0
17	35.244133916418086	37.0	37.0	37.0	33.0	37.0
18	35.22814445828144	37.0	37.0	37.0	33.0	37.0
19	35.25248341973413	37.0	37.0	37.0	33.0	37.0
20	35.14937299081931	37.0	37.0	37.0	33.0	37.0
21	35.229256566943725	37.0	37.0	37.0	33.0	37.0
22	35.28516898838657	37.0	37.0	37.0	33.0	37.0
23	35.2458339366909	37.0	37.0	37.0	33.0	37.0
24	35.243276665990905	37.0	37.0	37.0	33.0	37.0
25	35.26309768600307	37.0	37.0	37.0	33.0	37.0
26	35.16837730603261	37.0	37.0	37.0	33.0	37.0
27	35.14314634075704	37.0	37.0	37.0	33.0	37.0
28	35.14527788235976	37.0	37.0	37.0	33.0	37.0
29	35.17711778505025	37.0	37.0	37.0	33.0	37.0
30	35.138839815807	37.0	37.0	37.0	33.0	37.0
31	35.13860812650236	37.0	37.0	37.0	33.0	37.0
32	35.14946277042486	37.0	37.0	37.0	33.0	37.0
33	35.128758434938746	37.0	37.0	37.0	33.0	37.0
34	35.09911668452605	37.0	37.0	37.0	33.0	37.0
35	35.08095513915839	37.0	37.0	37.0	33.0	37.0
36	35.08447102435634	37.0	37.0	37.0	33.0	37.0
37	35.09211387529323	37.0	37.0	37.0	33.0	37.0
38	34.938075241101686	37.0	37.0	37.0	27.0	37.0
39	35.01070694199079	37.0	37.0	37.0	27.0	37.0
40	35.05149874018941	37.0	37.0	37.0	27.0	37.0
41	35.08735266008283	37.0	37.0	37.0	33.0	37.0
42	34.9660662052188	37.0	37.0	37.0	27.0	37.0
43	34.801083147499206	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	16.0
17	16.0
18	14.0
19	16.0
20	26.0
21	67.0
22	182.0
23	455.0
24	939.0
25	1650.0
26	2776.0
27	4311.0
28	5856.0
29	7999.0
30	10521.0
31	13325.0
32	16947.0
33	22414.0
34	32318.0
35	58516.0
36	166922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.69130296272698	20.56821801963567	13.549769758753511	25.190709258883835
2	15.673491847432594	22.79648990703467	38.03295780358539	23.497060441947347
3	17.9307248979119	26.610095861449796	29.992180485968316	25.46699875466999
4	12.899881259231371	17.69961481653103	38.82475600220105	30.575747922036552
5	14.184308841843087	36.9941208838947	35.22198731501057	13.599582959251643
6	30.17231892032784	39.33070752121405	17.493990558660837	13.002982999797272
7	26.65353760607026	31.817023371658603	23.7733499377335	17.756089084537635
8	24.36126154826378	36.341915491326134	20.66668597410872	18.63013698630137
9	25.710851747806192	15.722725824669118	20.230530858119263	38.335891569405426
10	14.602797648353558	28.493150684931507	34.20023748153726	22.703814185177677
11	34.23470126560283	23.446668018187612	24.255553303020648	18.063077413188914
12	22.823134177068553	25.433983028758433	30.61426626893336	21.128616525239654
13	27.94491586782125	22.149207912189752	26.189000550262097	23.716875669726896
14	21.780242694546615	20.708390048944366	27.654145790494944	29.857221466014078
15	23.552955486692344	28.931912305598196	23.95696371166266	23.558168496046804
16	23.466651220713025	28.25480031278056	24.79596860609922	23.482579860407192
17	22.4622780850879	27.76477743346173	26.444438008630428	23.32850647281995
18	23.34095977294448	27.459237162964467	27.184974948593936	22.014828115497117
19	24.37921746937357	26.639057024530103	26.821222740305252	22.160502765791072
20	23.87355556199137	26.709432650815256	27.061600393871814	22.355411393321557
21	23.197891627327756	27.01178719337369	26.9944104955255	22.79591068377306
22	22.94650873179067	26.842943612615482	27.193373685887224	23.017173969706622
23	22.96417504126966	26.790813519070927	27.335572996611546	22.909438443047872
24	23.057140374757452	27.168756697268964	26.389122187147034	23.384980740826553
25	23.32213501694228	26.9194010831475	26.98977670943265	22.768687190477568
26	23.234382692808943	27.043644472762026	26.9005763271453	22.821396507283733
27	23.191520171450087	26.90434127834574	26.726230125401834	23.17790842480234
28	23.020938920907064	26.71696255321614	27.27794028208173	22.984158243795072
29	23.836195661617772	26.63326479191404	26.929827101856414	22.600712444611776
30	23.313446668018187	26.725361290509426	26.922586811086337	23.038605230386054
31	23.256393176749977	26.642821975730545	26.962842827767965	23.137942019751513
32	22.680355643082624	26.78473167482406	27.213356888412637	23.321555793680673
33	22.778244374294072	26.845260505661905	27.46068522111848	22.91580989892554
34	23.244808711517855	26.399258594225145	27.233919314199657	23.122013380057343
35	23.169509687509052	27.008311853804052	26.91389846216224	22.908279996524662
36	23.58799849401952	26.871904775695793	26.555648874858818	22.984447855425874
37	23.235541139332156	26.421269078166183	26.891887978221206	23.45130180428046
38	23.129543282458222	26.897390599206467	26.792840800486545	23.180225317848766
39	22.933476208404528	26.704798864722406	27.27012076805004	23.091604158823017
40	23.14894726172203	26.79370963537896	27.350632801413305	22.706710301485707
41	23.033971444293204	26.22288511106606	27.40276289495786	23.340380549682877
42	22.864548640273394	26.90578933649975	27.061310782241016	23.16835124098584
43	23.269425700136118	26.15917055228938	27.34889513162849	23.222508615946015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.5
2	9.0
3	46.5
4	84.0
5	84.0
6	105.0
7	126.0
8	118.5
9	111.0
10	149.5
11	188.0
12	188.0
13	296.0
14	404.0
15	756.0
16	1108.0
17	1398.0
18	1688.0
19	1688.0
20	1691.5
21	1695.0
22	2188.0
23	2681.0
24	3592.0
25	4503.0
26	4503.0
27	6033.0
28	7563.0
29	9127.0
30	10691.0
31	12460.0
32	14229.0
33	14229.0
34	16418.5
35	18608.0
36	20208.5
37	21809.0
38	23464.0
39	25119.0
40	25119.0
41	25928.0
42	26737.0
43	27145.5
44	27554.0
45	27159.0
46	26764.0
47	26764.0
48	25901.5
49	25039.0
50	24683.0
51	24327.0
52	23339.5
53	22352.0
54	22352.0
55	21334.0
56	20316.0
57	18251.5
58	16187.0
59	14896.5
60	13606.0
61	13606.0
62	12034.5
63	10463.0
64	8971.5
65	7480.0
66	6330.5
67	5181.0
68	5181.0
69	4297.0
70	3413.0
71	2796.0
72	2179.0
73	1746.5
74	1314.0
75	1314.0
76	1025.0
77	736.0
78	594.5
79	453.0
80	351.5
81	250.0
82	250.0
83	194.5
84	139.0
85	124.0
86	109.0
87	80.0
88	51.0
89	51.0
90	35.0
91	19.0
92	10.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	345290.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.08006405779616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.61938723179472	67.28629334183587
2	6.222471350940382	9.343670952528107
3	1.6443619070554398	3.70376391947767
4	0.7226226542135854	2.1701822067188274
5	0.4230691594040625	1.5882029794467494
6	0.25550217180162316	1.1509871655463146
7	0.18712656241206255	0.9834632004968993
8	0.1383174145289394	0.8307904274513214
9	0.10515965624774569	0.7105854354638914
>10	0.6366473307799289	8.837938881321293
>50	0.03293536469963512	1.70360636358307
>100	0.011624246364326286	1.271304612147353
>500	7.749497576217523E-4	0.4192105139826855
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	847	0.24530105129021978	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.17202930869703725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0017376697848185583	0.0	0.0
8	0.0	0.0	0.0023168930464247442	0.0	0.0
9	0.0	0.0	0.0026065046772278375	2.8961163080309303E-4	0.0
10	0.0	0.0	0.0031857279388340237	2.8961163080309303E-4	0.0
11	0.0	0.0	0.0034753395696371166	2.8961163080309303E-4	0.0
12	0.0	0.0	0.004054562831243303	2.8961163080309303E-4	0.0
13	0.0	0.0	0.0046337860928494885	2.8961163080309303E-4	0.0
14	0.0	0.0	0.004923397723652582	2.8961163080309303E-4	0.0
15	0.0	0.0	0.005502620985258768	2.8961163080309303E-4	0.0
16	0.0	0.0	0.006081844246864954	8.688348924092791E-4	0.0
17	0.0	0.0	0.006081844246864954	0.0011584465232123721	0.0
18	0.0	0.0	0.006081844246864954	0.0014480581540154652	0.0
19	0.0	0.0	0.0063714558776680474	0.0014480581540154652	0.0
20	0.0	0.0	0.00666106750847114	0.0014480581540154652	0.0
21	0.0	0.0	0.006950679139274233	0.0017376697848185583	0.0
22	0.0	0.0	0.006950679139274233	0.0017376697848185583	0.0
23	0.0	0.0	0.0072402907700773265	0.0017376697848185583	0.0
24	0.0	0.0	0.0072402907700773265	0.0017376697848185583	0.0
25	0.0	0.0	0.0072402907700773265	0.0017376697848185583	0.0
26	0.0	0.0	0.0072402907700773265	0.0020272814156216514	0.0
27	0.0	0.0	0.0072402907700773265	0.0028961163080309304	0.0
28	0.0	0.0	0.0072402907700773265	0.00666106750847114	0.0
29	0.0	0.0	0.0072402907700773265	0.014770193170957745	0.0
30	0.0	0.0	0.0072402907700773265	0.031567667757537145	0.0
31	0.0	0.0	0.0072402907700773265	0.074719800747198	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	115	0.0	25.73913	1
GTATCAA	380	0.0	23.855263	1
AGGTCGC	55	5.133084E-4	20.181818	35
GGTCGCC	60	9.218984E-4	18.5	36
ACGGAAC	80	1.6113383E-5	18.5	34
TAGGGGT	50	0.0070251585	18.5	4
TAAGGGA	70	1.21606456E-4	18.5	4
TACCGCA	50	0.0070251585	18.5	7
GTATTAG	110	7.768758E-7	16.818182	1
GTATAAA	80	3.373999E-4	16.1875	1
GCTCGGA	80	3.373999E-4	16.1875	11
CTAAGAC	115	1.2359724E-6	16.086956	3
GTCGCCC	70	0.0025875703	15.857143	37
AAGACTC	70	0.0025875703	15.857143	5
CCGAGGT	70	0.0025875703	15.857143	32
TTCATAC	85	5.3506636E-4	15.235294	3
ATTAGAG	75	0.0040966775	14.8	3
GAGGTCG	75	0.0040966775	14.8	34
TCTAAGC	100	1.0898482E-4	14.8	3
AGTGTGA	75	0.0040966775	14.8	8
>>END_MODULE