FastQCFastQC Report
Fri 10 Feb 2017
ERR1633042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306852
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8140.2652744645627208No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG6560.21378384367708214No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT5870.19129743329031587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.18706086321744686No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG5020.16359678281386467No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT4580.1492576225672311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3190.1039589117880933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACC200.001839833937.04
GGTATCA1050.029.952381
CTAGACA1400.027.7500024
GTATCAA4300.027.104651
TACTGAC358.8545214E-426.4285727
TCTAGAC1300.025.6153853
TTTCGGT451.320126E-424.66666830
CCAACGG451.320126E-424.66666831
TCGGTTG400.001927979523.12500232
CTCTAGT1400.022.46428727
GTTCTAG1500.022.2000031
ATTTCGG502.6958514E-422.229
GGCTACT1201.0913936E-1121.58333412
CTAGTTT1400.021.14285929
CGGTTGG450.003819514520.55555733
TGGGGCG450.003819514520.55555737
TCTAGTT1550.020.29032328
GACATGT1650.020.181827
TTAACGG555.1312934E-420.18181835
TGCTAGA555.1312934E-420.18181816
Produced by FastQC (version 0.11.5)