FastQCFastQC Report
Fri 10 Feb 2017
ERR1633041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348059
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9900.2844345355241496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.21576801634205695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3890.11176266092817597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACA250.00549114629.59999817
CTCTAAT754.6020432E-1027.1333331
GTATCAA4900.026.428571
ATGCTAG901.4188117E-1024.66666615
TCTATAC400.001928497523.1250023
GGTATCA1550.022.677421
ATCGTAC502.696861E-422.19999925
CATCGTA502.696861E-422.19999924
CTAATAC1600.021.9687523
CTAGAGG1059.749783E-1021.14285718
GTTCTAG450.00382053120.5555551
GACATCG555.133197E-420.18181822
TCTAATA1254.0927262E-1019.242
TAATACT1850.019.04
TAATATC609.219188E-418.54
TAGAGGT1205.136826E-918.519
CCCTACA801.6113992E-518.52
TCGTCTG609.219188E-418.523
GATGCTA1306.91216E-1018.514
ACTAATA609.219188E-418.52
Produced by FastQC (version 0.11.5)