##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633041.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348059
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74038309596936	33.0	33.0	33.0	27.0	33.0
2	31.24649843848313	33.0	33.0	33.0	27.0	33.0
3	31.667484535667803	33.0	33.0	33.0	27.0	33.0
4	31.800134459962248	33.0	33.0	33.0	27.0	33.0
5	31.91251483225545	33.0	33.0	33.0	33.0	33.0
6	34.80763318862606	37.0	37.0	37.0	27.0	37.0
7	34.98137672061346	37.0	37.0	37.0	27.0	37.0
8	35.15405146828555	37.0	37.0	37.0	33.0	37.0
9	35.22757635918048	37.0	37.0	37.0	33.0	37.0
10	35.26042423841935	37.0	37.0	37.0	33.0	37.0
11	35.29105122982023	37.0	37.0	37.0	33.0	37.0
12	35.25771492764158	37.0	37.0	37.0	33.0	37.0
13	35.28017376364352	37.0	37.0	37.0	33.0	37.0
14	35.248839995518004	37.0	37.0	37.0	33.0	37.0
15	35.27238772736806	37.0	37.0	37.0	33.0	37.0
16	35.280081825207795	37.0	37.0	37.0	33.0	37.0
17	35.23953697505308	37.0	37.0	37.0	33.0	37.0
18	35.24476022743271	37.0	37.0	37.0	33.0	37.0
19	35.23977256729462	37.0	37.0	37.0	33.0	37.0
20	35.24975937987525	37.0	37.0	37.0	33.0	37.0
21	35.26249285322316	37.0	37.0	37.0	33.0	37.0
22	35.26203028796842	37.0	37.0	37.0	33.0	37.0
23	35.2380860716143	37.0	37.0	37.0	33.0	37.0
24	35.22620590187296	37.0	37.0	37.0	33.0	37.0
25	35.224203367819825	37.0	37.0	37.0	33.0	37.0
26	35.19405330705426	37.0	37.0	37.0	33.0	37.0
27	35.14242412924246	37.0	37.0	37.0	33.0	37.0
28	35.11196664933244	37.0	37.0	37.0	33.0	37.0
29	35.15420374131972	37.0	37.0	37.0	33.0	37.0
30	35.11416167948538	37.0	37.0	37.0	33.0	37.0
31	35.112684918361545	37.0	37.0	37.0	33.0	37.0
32	35.12681183362591	37.0	37.0	37.0	33.0	37.0
33	35.113713479611214	37.0	37.0	37.0	33.0	37.0
34	35.04921292079791	37.0	37.0	37.0	27.0	37.0
35	35.040047807986575	37.0	37.0	37.0	27.0	37.0
36	35.03003801079702	37.0	37.0	37.0	27.0	37.0
37	35.06142062121652	37.0	37.0	37.0	27.0	37.0
38	34.915583277547775	37.0	37.0	37.0	27.0	37.0
39	34.99605239341606	37.0	37.0	37.0	27.0	37.0
40	35.02938869559471	37.0	37.0	37.0	27.0	37.0
41	35.05234457376479	37.0	37.0	37.0	27.0	37.0
42	34.962213302917036	37.0	37.0	37.0	27.0	37.0
43	34.76179900534105	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	20.0
17	20.0
18	8.0
19	19.0
20	19.0
21	87.0
22	195.0
23	438.0
24	930.0
25	1627.0
26	2760.0
27	4378.0
28	6082.0
29	8050.0
30	10674.0
31	13665.0
32	17346.0
33	22998.0
34	32548.0
35	58441.0
36	167746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63420282193536	20.638167666976003	13.710031919875654	25.017597591212986
2	15.850760934209429	23.207846945489127	37.839848991119325	23.101543129182122
3	17.67775003663172	26.860675919887143	30.400018387687144	25.06155565579399
4	12.781741026665019	18.093484150675604	39.16491169600556	29.95986312665381
5	13.995902993458007	37.074748821320526	35.33854892417665	13.590799261044822
6	29.567688236764454	39.72889653765597	17.6358031253322	13.067612100247372
7	26.357600291904532	32.38042975472549	23.742813718363838	17.519156235006133
8	24.096776695905003	36.594657802269154	20.850487991978373	18.458077509847467
9	25.854237356310282	15.641888300546746	20.30575276030788	38.19812158283509
10	14.681993570055651	28.469023929850973	34.32377843986221	22.525204060231168
11	34.07813043190953	23.629614519377462	24.324324324324326	17.96793072438868
12	22.782631680261105	25.65944279561798	30.66692715890122	20.89099836521969
13	27.84211872125128	22.237321833367332	26.18406649447364	23.73649295090775
14	21.953461913066462	20.93955335158694	27.502808431903787	29.60417630344281
15	23.69512065483151	29.0295610801617	24.080400162041492	23.1949181029653
16	23.10930043469642	28.472184313579017	25.22790676293387	23.190608488790694
17	22.322364886412934	27.77517604773903	26.50498909667614	23.397469969171894
18	22.9986870042148	27.374669237112098	27.56572879885307	22.06091495982003
19	24.392128920671496	26.81872900858762	27.170968140458946	21.618173930281934
20	24.067758627129308	26.623072525060405	27.080466242792173	22.228702605018114
21	23.212156559663736	27.02214279762914	27.246530042320412	22.519170600386715
22	22.8817528062771	26.9092309062544	27.21923581921456	22.989780468253944
23	22.891808572684518	26.783390172355837	27.614571092831962	22.710230162127683
24	23.124815045725004	27.268940036028372	26.49206025415231	23.114184664094306
25	23.345467291464953	26.886533604934797	27.336170017152266	22.431829086447987
26	23.088614286658295	27.190217750438862	27.135916611838795	22.58525135106404
27	23.1630269580732	27.08879816352975	26.714723653173746	23.03345122522331
28	22.975127780060276	26.938823590253374	27.127010075877937	22.959038553808405
29	23.593701067922392	26.89946244745862	26.856653613324177	22.65018287129481
30	23.301221919272308	26.926469362952833	27.277559264377594	22.49474945339727
31	23.306968071505118	26.808098626956923	27.18504621342933	22.699887088108625
32	22.452515234486107	26.72305557391132	27.468044210895282	23.356384980707293
33	22.621739417742397	26.794882476821456	27.608537632987513	22.974840472448637
34	23.035749686116432	26.54837254603386	27.357143472802026	23.058734295047678
35	23.009317385845502	27.011225108386792	27.29278656779454	22.68667093797316
36	23.646278360852612	26.82045285425747	26.73857018493991	22.79469859995001
37	23.027417765378857	26.646919056826572	27.179012753584882	23.14665042420969
38	23.2253727097992	26.894578218060733	26.85952668944058	23.020522382699486
39	22.804467058745786	26.772472483113496	27.363464240258118	23.0595962178826
40	22.766255146397594	27.02788894986195	27.61916801461821	22.58668788912225
41	23.112173510812823	26.49091102370575	27.22526927905901	23.171646186422418
42	22.62432518624716	26.957785892621654	27.345651168336403	23.072237752794784
43	23.14779965465625	26.204465334900117	27.35972924130679	23.28800576913684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	14.5
2	20.0
3	66.5
4	113.0
5	113.0
6	124.0
7	135.0
8	141.5
9	148.0
10	190.0
11	232.0
12	232.0
13	367.5
14	503.0
15	955.0
16	1407.0
17	1653.0
18	1899.0
19	1899.0
20	1909.0
21	1919.0
22	2359.5
23	2800.0
24	3831.5
25	4863.0
26	4863.0
27	6142.5
28	7422.0
29	9086.0
30	10750.0
31	12556.5
32	14363.0
33	14363.0
34	16269.5
35	18176.0
36	19831.0
37	21486.0
38	23214.0
39	24942.0
40	24942.0
41	26377.5
42	27813.0
43	28238.5
44	28664.0
45	28130.5
46	27597.0
47	27597.0
48	26730.0
49	25863.0
50	25605.0
51	25347.0
52	24448.5
53	23550.0
54	23550.0
55	21896.0
56	20242.0
57	17882.5
58	15523.0
59	14376.5
60	13230.0
61	13230.0
62	11475.5
63	9721.0
64	8333.5
65	6946.0
66	5842.5
67	4739.0
68	4739.0
69	3916.0
70	3093.0
71	2497.0
72	1901.0
73	1490.0
74	1079.0
75	1079.0
76	877.0
77	675.0
78	525.5
79	376.0
80	305.5
81	235.0
82	235.0
83	175.0
84	115.0
85	109.0
86	103.0
87	67.5
88	32.0
89	32.0
90	27.5
91	23.0
92	14.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	348059.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.67789549710022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63485465686813	62.64524994838793
2	6.543679040248983	9.249869267465655
3	1.806837697604622	3.83110457914562
4	0.8337554944411891	2.3571233482499
5	0.4842878279270364	1.7114222246372366
6	0.3157232396832579	1.3388792484203538
7	0.2327849911083517	1.1516927292394643
8	0.15426448444560484	0.8722471288448401
9	0.14077991302704979	0.8955025182915015
>10	0.7753660246424454	10.486486174441936
>50	0.05559295621303505	2.6368930791427814
>100	0.02125613031659812	2.3205352483354202
>500	8.175434737153124E-4	0.5029945053974061
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	990	0.2844345355241496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.21576801634205695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	389	0.11176266092817597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.873076116405552E-4	0.0	2.873076116405552E-4	0.0	0.0
3	2.873076116405552E-4	0.0	2.873076116405552E-4	0.0	0.0
4	2.873076116405552E-4	0.0	2.873076116405552E-4	0.0	0.0
5	2.873076116405552E-4	0.0	2.873076116405552E-4	0.0	0.0
6	2.873076116405552E-4	0.0	0.001436538058202776	0.0	0.0
7	2.873076116405552E-4	0.0	0.0025857685047649967	0.0	0.0
8	2.873076116405552E-4	0.0	0.003160383728046107	0.0	0.0
9	2.873076116405552E-4	0.0	0.004884229397889438	2.873076116405552E-4	0.0
10	2.873076116405552E-4	0.0	0.005746152232811104	5.746152232811104E-4	0.0
11	2.873076116405552E-4	0.0	0.009481151184138321	5.746152232811104E-4	0.0
12	2.873076116405552E-4	0.0	0.009768458795778876	5.746152232811104E-4	0.0
13	2.873076116405552E-4	0.0	0.010343074019059987	8.619228349216655E-4	0.0
14	2.873076116405552E-4	0.0	0.010630381630700542	8.619228349216655E-4	0.0
15	2.873076116405552E-4	0.0	0.012066919688903319	8.619228349216655E-4	0.0
16	2.873076116405552E-4	0.0	0.012641534912184428	8.619228349216655E-4	0.0
17	2.873076116405552E-4	0.0	0.012641534912184428	0.0011492304465622208	0.0
18	2.873076116405552E-4	0.0	0.012928842523824985	0.0011492304465622208	0.0
19	2.873076116405552E-4	0.0	0.012928842523824985	0.0011492304465622208	0.0
20	2.873076116405552E-4	0.0	0.01321615013546554	0.0011492304465622208	0.0
21	2.873076116405552E-4	0.0	0.013503457747106094	0.0011492304465622208	2.873076116405552E-4
22	2.873076116405552E-4	0.0	0.013790765358746649	0.0011492304465622208	2.873076116405552E-4
23	2.873076116405552E-4	0.0	0.013790765358746649	0.0011492304465622208	2.873076116405552E-4
24	2.873076116405552E-4	0.0	0.013790765358746649	0.001436538058202776	2.873076116405552E-4
25	2.873076116405552E-4	0.0	0.013790765358746649	0.001436538058202776	2.873076116405552E-4
26	2.873076116405552E-4	0.0	0.014078072970387205	0.001436538058202776	2.873076116405552E-4
27	2.873076116405552E-4	0.0	0.014078072970387205	0.001723845669843331	2.873076116405552E-4
28	2.873076116405552E-4	0.0	0.014078072970387205	0.008044613125935546	2.873076116405552E-4
29	2.873076116405552E-4	0.0	0.014078072970387205	0.021835378484682196	2.873076116405552E-4
30	2.873076116405552E-4	0.0	0.014078072970387205	0.037349989513272175	2.873076116405552E-4
31	2.873076116405552E-4	0.0	0.014078072970387205	0.10544189347208376	2.873076116405552E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACA	25	0.005491146	29.599998	17
CTCTAAT	75	4.6020432E-10	27.133333	1
GTATCAA	490	0.0	26.42857	1
ATGCTAG	90	1.4188117E-10	24.666666	15
TCTATAC	40	0.0019284975	23.125002	3
GGTATCA	155	0.0	22.67742	1
ATCGTAC	50	2.696861E-4	22.199999	25
CATCGTA	50	2.696861E-4	22.199999	24
CTAATAC	160	0.0	21.968752	3
CTAGAGG	105	9.749783E-10	21.142857	18
GTTCTAG	45	0.003820531	20.555555	1
GACATCG	55	5.133197E-4	20.181818	22
TCTAATA	125	4.0927262E-10	19.24	2
TAATACT	185	0.0	19.0	4
TAATATC	60	9.219188E-4	18.5	4
TAGAGGT	120	5.136826E-9	18.5	19
CCCTACA	80	1.6113992E-5	18.5	2
TCGTCTG	60	9.219188E-4	18.5	23
GATGCTA	130	6.91216E-10	18.5	14
ACTAATA	60	9.219188E-4	18.5	2
>>END_MODULE