FastQCFastQC Report
Fri 10 Feb 2017
ERR1633040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469887
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30510.6493050456811956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22040.4690489415540332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11200.23835517901112396No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9740.20728387889003103No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9500.2021762679112212No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9430.2006865480424017No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8570.18238427536833324No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8470.18025610412716248No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8380.17834075001010882No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8100.1723818705348307No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7930.16876397942484045No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7670.1632307341977965No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7540.1604641115842745No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7520.16003847733604037No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6970.14833353550960124No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6840.14556691289607926No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6670.141949021786089No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6500.13833113067609873No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6430.13684141080727918No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6270.13343633682140602No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6170.13130816558023525No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6160.13109534845611817No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6010.12790309159436206No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6000.12769027447024497No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5940.1264133717255425No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5880.12513646898084008No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5750.12236984636731811No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5570.11853913813321074No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5560.11832632100909368No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5510.1172622353885083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.11641096689204No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5290.11258025865793266No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5130.10917518467205944No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5120.10896236754794238No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5080.10811109905147406No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5050.10747264767912285No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4980.10598292781030332No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4970.10577011068618625No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4950.1053444764379521No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4940.10513165931383503No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4900.10428039081736673No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4890.10406757369324966No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4880.10385475656913257No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG4840.10300348807266428No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4750.1010881339556106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGA200.001840828737.030
GGTATCA5100.032.2843131
CCCGAAT303.5962687E-430.83333228
AGCCCGA250.00549307729.626
TATATAA250.00549307729.62
TGGTGCG250.00549307729.67
TAATCGA250.00549307729.631
TCGGCGT454.0016275E-628.77777911
CGCCACA405.93277E-527.75000215
CTCGCCA451.3215859E-424.66666813
TCATCGG601.3345962E-624.6666668
GTATCAA15600.024.1923081
GCCGAGT952.8012437E-1023.36842212
GTAAATC652.6772432E-622.7692316
AACAGTG502.698808E-422.27
TGACTCG502.698808E-422.210
TGCCGAG1005.311449E-1022.211
CGGCGTA603.720254E-521.58333212
ATCGGCG603.720254E-521.58333210
ATTAGAG1900.021.4210533
Produced by FastQC (version 0.11.5)