##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633040.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469887
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744370455024292	33.0	33.0	33.0	27.0	33.0
2	31.238078516749773	33.0	33.0	33.0	27.0	33.0
3	31.677901282648808	33.0	33.0	33.0	27.0	33.0
4	31.800360512208254	33.0	33.0	33.0	27.0	33.0
5	31.927493205813313	33.0	33.0	33.0	33.0	33.0
6	34.786940264361434	37.0	37.0	37.0	27.0	37.0
7	34.97540898130827	37.0	37.0	37.0	27.0	37.0
8	35.17914519873927	37.0	37.0	37.0	33.0	37.0
9	35.24356707038075	37.0	37.0	37.0	33.0	37.0
10	35.224747652095076	37.0	37.0	37.0	33.0	37.0
11	35.29147433319075	37.0	37.0	37.0	33.0	37.0
12	35.23659305322344	37.0	37.0	37.0	33.0	37.0
13	35.27195687473797	37.0	37.0	37.0	33.0	37.0
14	35.25211593425654	37.0	37.0	37.0	33.0	37.0
15	35.27553858693686	37.0	37.0	37.0	33.0	37.0
16	35.26272486789377	37.0	37.0	37.0	33.0	37.0
17	35.235665170562285	37.0	37.0	37.0	33.0	37.0
18	35.24638476910406	37.0	37.0	37.0	33.0	37.0
19	35.24663163696804	37.0	37.0	37.0	33.0	37.0
20	35.236473875633926	37.0	37.0	37.0	33.0	37.0
21	35.241406976570964	37.0	37.0	37.0	33.0	37.0
22	35.22883586904937	37.0	37.0	37.0	33.0	37.0
23	35.2391915503089	37.0	37.0	37.0	33.0	37.0
24	35.22943814151062	37.0	37.0	37.0	33.0	37.0
25	35.22936152734594	37.0	37.0	37.0	33.0	37.0
26	35.187059867585184	37.0	37.0	37.0	33.0	37.0
27	35.11955214764401	37.0	37.0	37.0	33.0	37.0
28	35.10864952637549	37.0	37.0	37.0	33.0	37.0
29	35.15214934654502	37.0	37.0	37.0	33.0	37.0
30	35.095771962195165	37.0	37.0	37.0	33.0	37.0
31	35.07234930951484	37.0	37.0	37.0	27.0	37.0
32	35.09843856076035	37.0	37.0	37.0	33.0	37.0
33	35.08910440169658	37.0	37.0	37.0	33.0	37.0
34	35.03230776761221	37.0	37.0	37.0	27.0	37.0
35	35.0149078395444	37.0	37.0	37.0	27.0	37.0
36	35.00760182767346	37.0	37.0	37.0	27.0	37.0
37	35.013965059684566	37.0	37.0	37.0	27.0	37.0
38	34.864716410541256	37.0	37.0	37.0	27.0	37.0
39	34.94315867431957	37.0	37.0	37.0	27.0	37.0
40	34.9618206079334	37.0	37.0	37.0	27.0	37.0
41	34.98156790888022	37.0	37.0	37.0	27.0	37.0
42	34.869332413963356	37.0	37.0	37.0	27.0	37.0
43	34.661761231955765	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	24.0
17	23.0
18	17.0
19	17.0
20	40.0
21	91.0
22	204.0
23	522.0
24	1209.0
25	2232.0
26	3776.0
27	5625.0
28	8197.0
29	11117.0
30	14765.0
31	19041.0
32	23919.0
33	31334.0
34	44606.0
35	80456.0
36	222663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4729498794391	20.97355321598597	13.627318908588663	23.92617799598627
2	16.56100296454254	23.155992823806574	38.3832708715074	21.89973334014348
3	17.034946699951263	28.696686650194618	30.21130612253584	24.05706052731827
4	11.680893491413894	18.69768689067797	41.43740941971154	28.184010198196585
5	12.67134438705476	37.9980718768555	36.132942601093454	13.197641134996285
6	27.13652431329234	42.43935244005473	17.270748073472983	13.153375173179935
7	25.92751023118324	32.502708097904396	24.267749480194176	17.302032190718194
8	23.74294245212147	35.67921649247585	20.673693888105014	19.90414716729767
9	26.17735753489669	14.74141655334155	20.747328613049522	38.333897298712245
10	15.688878389910766	27.491928910567857	34.08393933009426	22.735253369427117
11	33.436336821406	24.611661952767367	24.078342239729977	17.873658986096657
12	23.17812580471475	27.363387367601145	29.087844524321802	20.3706423033623
13	27.941398676703123	23.037240868549247	25.831316891082324	23.19004356366531
14	22.22513072291849	21.467288943937586	28.444072723867652	27.863507609276272
15	24.317761504361687	28.52515604815626	25.676598841849213	21.480483605632845
16	22.200656753645028	28.55282227429148	25.659360654795726	23.58716031726777
17	21.82439607820604	27.23676117875149	26.280361022969352	24.658481720073127
18	22.53158738164708	27.12311683447297	28.457054568438796	21.88824121544116
19	25.420154207288135	25.512729656279063	28.2165712181865	20.850544918246303
20	24.432682751384906	25.965391679276077	28.48280544045696	21.11912012888205
21	22.750150568115313	27.45617563371619	28.21593276681415	21.577741031354346
22	22.050620681142487	27.135034593423523	28.4072553613954	22.407089364038587
23	22.330688016480558	26.813893553130857	29.4604873086508	21.394931121737777
24	22.365590024835758	28.032910040073467	26.3133476772075	23.28815225788328
25	22.68290035689432	27.443619423393283	28.708178774896943	21.16530144481546
26	22.37644369816573	27.703256314816116	28.462374996541723	21.457924990476435
27	22.169585453523933	27.628344687126905	27.49980314416019	22.70226671518897
28	21.74054613130391	27.054589720507273	29.38131933847925	21.82354480970957
29	24.288818375481767	26.77792745915507	27.34402100930649	21.589233156056668
30	23.21962514391758	26.607248125613182	28.454075128701156	21.719051601768086
31	23.0902323324544	26.857308246450746	28.12633675756086	21.926122663534
32	21.07783360680334	26.389961841889647	29.00654838290908	23.525656168397933
33	20.99398365990121	26.895615328791816	29.703950098640735	22.406450912666237
34	22.551379374189963	26.113299580537447	29.06273210367599	22.272588941596595
35	22.293232202635956	27.664097963978573	28.22955306275764	21.813116770627833
36	23.858927784765275	27.362748916228796	26.971165407853377	21.807157891152553
37	22.677367111667273	26.27333805787349	27.829669686541656	23.21962514391758
38	22.589473639406922	27.232292019145028	27.576417308842338	22.601817032605712
39	21.77417123691441	26.888805180820068	28.676256206279383	22.66076737598614
40	21.797793937691402	27.824774892686964	29.057198858448945	21.320232311172685
41	22.296211642373592	25.99667579652129	28.41257578949833	23.29453677160679
42	21.347047268811437	27.957147143887784	28.34170768716734	22.354097900133436
43	22.898696920749032	25.931553756541465	28.359158691344938	22.810590631364562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	26.0
2	34.0
3	158.5
4	283.0
5	283.0
6	370.0
7	457.0
8	439.5
9	422.0
10	571.0
11	720.0
12	720.0
13	1043.5
14	1367.0
15	2498.0
16	3629.0
17	3994.5
18	4360.0
19	4360.0
20	3483.5
21	2607.0
22	2304.5
23	2002.0
24	2797.5
25	3593.0
26	3593.0
27	4939.5
28	6286.0
29	8517.0
30	10748.0
31	13987.0
32	17226.0
33	17226.0
34	21290.0
35	25354.0
36	28591.5
37	31829.0
38	34647.0
39	37465.0
40	37465.0
41	40290.0
42	43115.0
43	44457.0
44	45799.0
45	44145.5
46	42492.0
47	42492.0
48	40983.0
49	39474.0
50	38230.0
51	36986.0
52	34317.5
53	31649.0
54	31649.0
55	30638.5
56	29628.0
57	23875.0
58	18122.0
59	16150.5
60	14179.0
61	14179.0
62	11896.5
63	9614.0
64	7155.5
65	4697.0
66	3754.0
67	2811.0
68	2811.0
69	2149.0
70	1487.0
71	1085.5
72	684.0
73	543.5
74	403.0
75	403.0
76	273.5
77	144.0
78	111.5
79	79.0
80	65.0
81	51.0
82	51.0
83	40.0
84	29.0
85	25.5
86	22.0
87	19.0
88	16.0
89	16.0
90	11.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	469887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.803754919859664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29376231779938	29.68543199901451
2	5.724707805598726	3.984826549077314
3	1.8845501696432339	1.9676826671532917
4	1.0651614271616985	1.4828646904409482
5	0.7711596405794944	1.3419625567407893
6	0.5882256528126626	1.2283476874839823
7	0.46531028651118844	1.1336181621397559
8	0.3796171819472047	1.0569682691086628
9	0.30125068930740184	0.9436189644084244
>10	2.7626450475350195	20.26114605880826
>50	0.42476936746166716	10.470033209480743
>100	0.3169011062841706	20.377993854386318
>500	0.02011103174495698	4.7133496339582495
>1k	0.0018282756131779076	1.352155697798751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3051	0.6493050456811956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2204	0.4690489415540332	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.23835517901112396	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	974	0.20728387889003103	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	950	0.2021762679112212	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	943	0.2006865480424017	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	857	0.18238427536833324	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	847	0.18025610412716248	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	838	0.17834075001010882	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	810	0.1723818705348307	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	793	0.16876397942484045	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	767	0.1632307341977965	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	754	0.1604641115842745	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	752	0.16003847733604037	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	697	0.14833353550960124	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	684	0.14556691289607926	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	667	0.141949021786089	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	650	0.13833113067609873	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	643	0.13684141080727918	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	627	0.13343633682140602	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	617	0.13130816558023525	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	616	0.13109534845611817	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	601	0.12790309159436206	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	600	0.12769027447024497	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	594	0.1264133717255425	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	588	0.12513646898084008	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	575	0.12236984636731811	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	557	0.11853913813321074	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	556	0.11832632100909368	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	551	0.1172622353885083	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	547	0.11641096689204	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	529	0.11258025865793266	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	513	0.10917518467205944	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	512	0.10896236754794238	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	508	0.10811109905147406	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	505	0.10747264767912285	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	498	0.10598292781030332	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	497	0.10577011068618625	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	495	0.1053444764379521	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	494	0.10513165931383503	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	490	0.10428039081736673	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	489	0.10406757369324966	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	488	0.10385475656913257	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG	484	0.10300348807266428	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	475	0.1010881339556106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.384513723512249E-4	0.0	0.0
2	0.0	0.0	6.384513723512249E-4	0.0	0.0
3	0.0	0.0	8.512684964682998E-4	0.0	0.0
4	0.0	0.0	8.512684964682998E-4	0.0	0.0
5	0.0	0.0	8.512684964682998E-4	0.0	0.0
6	0.0	0.0	0.0021281712411707495	0.0	0.0
7	0.0	0.0	0.005958879475278098	0.0	0.0
8	0.0	0.0	0.007022965095863473	0.0	0.0
9	0.0	0.0	0.008725502088800073	8.512684964682998E-4	0.0
10	0.0	0.0	0.010640856205853748	8.512684964682998E-4	0.0
11	0.0	0.0	0.017876638425834295	8.512684964682998E-4	0.0
12	0.0	0.0	0.019791992542887972	0.0010640856205853747	0.0
13	0.0	0.0	0.021281712411707496	0.0010640856205853747	0.0
14	0.0	0.0	0.02277143228052702	0.0010640856205853747	0.0
15	0.0	0.0	0.02447396927346362	0.0010640856205853747	0.0
16	0.0	0.0	0.02532523776993192	0.0012769027447024498	0.0
17	0.0	0.0	0.025963689142283145	0.0012769027447024498	0.0
18	0.0	0.0	0.026814957638751444	0.0012769027447024498	0.0
19	0.0	0.0	0.02745340901110267	0.0012769027447024498	0.0
20	0.0	0.0	0.028091860383453893	0.0017025369929365996	0.0
21	0.0	0.0	0.029368763128156342	0.0019153541170536747	0.0
22	0.0	0.0	0.02958158025227342	0.0021281712411707495	0.0
23	0.0	0.0	0.03000721450050757	0.0027666226135219744	0.0
24	0.0	0.0	0.03043284874874172	0.002979439737639049	0.0
25	0.0	0.0	0.03043284874874172	0.004043525358224424	0.0
26	0.0	0.0	0.03043284874874172	0.004469159606458574	0.0
27	0.0	0.0	0.030645665872858795	0.005746062351161024	0.0
28	0.0	0.0	0.030645665872858795	0.012130576074673273	0.0
29	0.0	0.0	0.030645665872858795	0.029368763128156342	0.0
30	0.0	0.0	0.030645665872858795	0.06001442900101514	0.0
31	0.0	0.0	0.030645665872858795	0.15003607250253784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGA	20	0.0018408287	37.0	30
GGTATCA	510	0.0	32.284313	1
CCCGAAT	30	3.5962687E-4	30.833332	28
AGCCCGA	25	0.005493077	29.6	26
TATATAA	25	0.005493077	29.6	2
TGGTGCG	25	0.005493077	29.6	7
TAATCGA	25	0.005493077	29.6	31
TCGGCGT	45	4.0016275E-6	28.777779	11
CGCCACA	40	5.93277E-5	27.750002	15
CTCGCCA	45	1.3215859E-4	24.666668	13
TCATCGG	60	1.3345962E-6	24.666666	8
GTATCAA	1560	0.0	24.192308	1
GCCGAGT	95	2.8012437E-10	23.368422	12
GTAAATC	65	2.6772432E-6	22.76923	16
AACAGTG	50	2.698808E-4	22.2	7
TGACTCG	50	2.698808E-4	22.2	10
TGCCGAG	100	5.311449E-10	22.2	11
CGGCGTA	60	3.720254E-5	21.583332	12
ATCGGCG	60	3.720254E-5	21.583332	10
ATTAGAG	190	0.0	21.421053	3
>>END_MODULE