FastQCFastQC Report
Fri 10 Feb 2017
ERR1633039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451074
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13260.29396507003285494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.19420316843799465No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5700.12636507535348968No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5460.1210444406017638No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5290.11727565765262463No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5110.1132851815888302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4690.10397407077330993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2600.029.1730771
CGTTCGC451.3214716E-424.66666634
GTATCAA8700.023.6034491
GTTCTAT400.001929377923.1250021
GTACATG1155.456968E-1222.521741
TTCGCCG603.7198282E-521.58333424
CTCGGTA450.003822259420.55555527
GCGAACT1101.7462298E-920.18181828
CCGTTCG555.1364396E-420.18181833
AGCTTCG656.890255E-519.92307721
CCGCTCT656.890255E-519.92307728
ATAATAC759.245319E-619.7333343
GTCGCCA951.6704871E-719.47368412
CTCGTCT951.6704871E-719.47368413
CGAACTA1153.0358933E-919.30434829
CATGCTA1153.0358933E-919.3043484
AACCGTG609.224959E-418.58
TTTGTCG500.00702841918.536
CGGCAGC701.2171141E-418.517
ACATTAT609.224959E-418.51
Produced by FastQC (version 0.11.5)