##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451074
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720059236400235	33.0	33.0	33.0	27.0	33.0
2	31.24088508759095	33.0	33.0	33.0	27.0	33.0
3	31.679265486372525	33.0	33.0	33.0	27.0	33.0
4	31.802362805215996	33.0	33.0	33.0	27.0	33.0
5	31.92795417159934	33.0	33.0	33.0	33.0	33.0
6	34.80819111720028	37.0	37.0	37.0	27.0	37.0
7	34.974068556378775	37.0	37.0	37.0	27.0	37.0
8	35.15771913255918	37.0	37.0	37.0	33.0	37.0
9	35.243503283275025	37.0	37.0	37.0	33.0	37.0
10	35.277240541463264	37.0	37.0	37.0	33.0	37.0
11	35.309654291757006	37.0	37.0	37.0	33.0	37.0
12	35.26404758421013	37.0	37.0	37.0	33.0	37.0
13	35.28862669983196	37.0	37.0	37.0	33.0	37.0
14	35.26849918195241	37.0	37.0	37.0	33.0	37.0
15	35.30292147186493	37.0	37.0	37.0	33.0	37.0
16	35.27379764739267	37.0	37.0	37.0	33.0	37.0
17	35.26648399154019	37.0	37.0	37.0	33.0	37.0
18	35.25116056345522	37.0	37.0	37.0	33.0	37.0
19	35.25786456324239	37.0	37.0	37.0	33.0	37.0
20	35.25650780138071	37.0	37.0	37.0	33.0	37.0
21	35.25305825651667	37.0	37.0	37.0	33.0	37.0
22	35.2645796476853	37.0	37.0	37.0	33.0	37.0
23	35.262010224486446	37.0	37.0	37.0	33.0	37.0
24	35.2611700075819	37.0	37.0	37.0	33.0	37.0
25	35.2532932512182	37.0	37.0	37.0	33.0	37.0
26	35.21472530006163	37.0	37.0	37.0	33.0	37.0
27	35.14763652970466	37.0	37.0	37.0	33.0	37.0
28	35.14810651910773	37.0	37.0	37.0	33.0	37.0
29	35.18230489897445	37.0	37.0	37.0	33.0	37.0
30	35.1541676088624	37.0	37.0	37.0	33.0	37.0
31	35.14622434456431	37.0	37.0	37.0	33.0	37.0
32	35.17215578818553	37.0	37.0	37.0	33.0	37.0
33	35.154934667039115	37.0	37.0	37.0	33.0	37.0
34	35.09486691762327	37.0	37.0	37.0	33.0	37.0
35	35.09863126671012	37.0	37.0	37.0	33.0	37.0
36	35.0795434895383	37.0	37.0	37.0	33.0	37.0
37	35.10141351529904	37.0	37.0	37.0	33.0	37.0
38	34.95071983754328	37.0	37.0	37.0	27.0	37.0
39	35.043884152046004	37.0	37.0	37.0	27.0	37.0
40	35.08520996554889	37.0	37.0	37.0	33.0	37.0
41	35.094079907066245	37.0	37.0	37.0	33.0	37.0
42	35.019493475571636	37.0	37.0	37.0	27.0	37.0
43	34.79648793767763	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	17.0
17	23.0
18	16.0
19	17.0
20	36.0
21	71.0
22	230.0
23	528.0
24	1142.0
25	2093.0
26	3565.0
27	5459.0
28	7638.0
29	10359.0
30	13537.0
31	17688.0
32	22351.0
33	29813.0
34	41402.0
35	76259.0
36	218821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.00768388335395	20.182719465098852	13.825891095474358	24.983705556072838
2	15.712499501190491	23.229891326035197	38.58302628836954	22.474582884404775
3	17.852503136957573	26.694511321867363	30.74573129907731	24.707254242097747
4	12.926260436203371	18.032739639172288	38.65330300571525	30.38769691890909
5	13.740317553217432	37.20476019455788	35.36160363931417	13.69331861291052
6	29.420228166553603	39.268944785112865	17.800626948128244	13.510200100205289
7	26.355985935788805	32.248810616439876	23.512550047220635	17.882653400550687
8	23.958153207677675	36.463640112265395	20.898344839205983	18.679861840850947
9	25.86205367633692	16.147461392144084	19.953267091430675	38.037217840088324
10	14.795133392747086	28.388246717833436	33.838350248517976	22.978269640901495
11	33.19699206782036	24.011802941424246	24.269632033768296	18.5215729569871
12	22.810226259992817	25.686472729529967	30.10570327706762	21.397597733409597
13	27.535614998869367	22.333807756598695	26.96564200109073	23.16493524344121
14	22.184164904206405	21.21270567578712	27.38708061205035	29.216048807956124
15	23.847306650350053	28.63742977870593	24.615694985745133	22.89956858519888
16	23.104413023140328	28.78862448290081	24.72809339487534	23.37886909908352
17	22.551067008960835	27.771496472862545	26.113675361470623	23.563761156705993
18	22.780962768858327	27.59547213982628	27.426541986458986	22.19702310485641
19	24.944244181664203	26.352660539068978	27.421443044821913	21.281652234444902
20	24.632987048688243	26.004602349060242	27.616089599489218	21.746321002762294
21	22.828848481623858	27.26359754718738	26.66945999991132	23.23809397127744
22	22.483672302105642	27.275790668493414	26.674337248433737	23.566199780967203
23	22.462389763098738	27.272465271773587	27.544039337226266	22.72110562790141
24	23.29839449846367	27.391071088114145	26.37349969184657	22.937034721575618
25	23.349827301063684	26.85789914736828	27.170486439032178	22.62178711253586
26	23.19996275555674	27.03148485614334	26.968745704695902	22.79980668360402
27	23.25427756864727	26.8335129047562	26.48168593179833	23.430523594798192
28	22.343118867414216	27.111294377419227	27.518322935926253	23.0272638192403
29	23.554450045890473	27.244753632441682	26.63465418091045	22.566142140757393
30	22.91020985470233	27.314143577328775	27.078483796450247	22.69716277151864
31	23.243192912914513	26.88583247981484	27.15962347641407	22.711351130856578
32	22.242470193360734	26.93637850995624	27.262932467843413	23.558218828839614
33	22.518256427991858	26.73264253758807	27.695677427650452	23.05342360676962
34	22.809117794419542	26.725104971689788	27.26714463702186	23.198632596868805
35	22.899790278313535	27.440065266452958	26.941255758478654	22.718888696754856
36	23.132124662472233	27.17625046001321	26.912435653573468	22.77918922394108
37	23.677046338294826	26.28304890106723	26.794051530347566	23.245853230290372
38	23.732691310073292	26.69872349104582	26.779863171009634	22.78872202787126
39	23.07448445266187	26.616918731738032	27.221919241632193	23.086677573967908
40	22.847027317025585	27.169156280344247	27.23743775965806	22.746378642972108
41	22.667234201040184	26.501638312117304	27.614981133915943	23.21614635292657
42	23.110842123465332	26.65238963008287	27.138119244292515	23.09864900215929
43	22.988024137946322	26.040516633634393	27.392622939916734	23.578836288502554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	14.5
2	19.0
3	67.0
4	115.0
5	115.0
6	159.0
7	203.0
8	197.0
9	191.0
10	256.5
11	322.0
12	322.0
13	471.0
14	620.0
15	1212.5
16	1805.0
17	2147.5
18	2490.0
19	2490.0
20	2495.0
21	2500.0
22	3127.5
23	3755.0
24	4862.5
25	5970.0
26	5970.0
27	7476.5
28	8983.0
29	11998.5
30	15014.0
31	16970.5
32	18927.0
33	18927.0
34	21101.5
35	23276.0
36	25516.0
37	27756.0
38	30022.0
39	32288.0
40	32288.0
41	33955.5
42	35623.0
43	34598.5
44	33574.0
45	34003.0
46	34432.0
47	34432.0
48	33741.0
49	33050.0
50	33871.0
51	34692.0
52	35208.5
53	35725.0
54	35725.0
55	31442.5
56	27160.0
57	24265.0
58	21370.0
59	18437.0
60	15504.0
61	15504.0
62	13756.0
63	12008.0
64	9951.5
65	7895.0
66	6755.5
67	5616.0
68	5616.0
69	4647.5
70	3679.0
71	3217.5
72	2756.0
73	2199.0
74	1642.0
75	1642.0
76	1260.0
77	878.0
78	701.5
79	525.0
80	390.0
81	255.0
82	255.0
83	210.5
84	166.0
85	157.5
86	149.0
87	113.0
88	77.0
89	77.0
90	60.5
91	44.0
92	26.0
93	8.0
94	4.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	451074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.15799718415925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.12810852983837	52.1348239625346
2	6.526247900841363	7.721595098821972
3	1.7877719828548455	3.1728302978293725
4	0.8440834583999614	1.9973714742088127
5	0.4687238247809554	1.3864381353270057
6	0.3509876047746109	1.2458234240958739
7	0.22083341880384735	0.9144843937436383
8	0.17284077235917888	0.8179931139625772
9	0.13181885722315712	0.7018325625983957
>10	1.119123087195836	13.612865982363637
>50	0.16177139535763968	6.760540172599988
>100	0.08542134427101254	8.558953421918309
>500	0.0018898527493586847	0.6779546155822225
>1k	3.7797054987173694E-4	0.2964933444135973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.29396507003285494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	876	0.19420316843799465	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	570	0.12636507535348968	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	546	0.1210444406017638	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	529	0.11727565765262463	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	511	0.1132851815888302	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.10397407077330993	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.2169311465524504E-4	0.0	0.0	0.0	0.0
3	2.2169311465524504E-4	0.0	0.0	0.0	0.0
4	2.2169311465524504E-4	0.0	2.2169311465524504E-4	0.0	0.0
5	2.2169311465524504E-4	0.0	4.4338622931049007E-4	0.0	0.0
6	2.2169311465524504E-4	0.0	0.0011084655732762252	0.0	0.0
7	2.2169311465524504E-4	0.0	0.0031037036051734307	0.0	0.0
8	2.2169311465524504E-4	0.0	0.0037687829491391657	0.0	0.0
9	2.2169311465524504E-4	0.0	0.004433862293104901	2.2169311465524504E-4	0.0
10	4.4338622931049007E-4	0.0	0.004877248522415391	2.2169311465524504E-4	0.0
11	4.4338622931049007E-4	0.0	0.008424338356899312	4.4338622931049007E-4	0.0
12	4.4338622931049007E-4	0.0	0.009976190159486026	6.650793439657351E-4	0.0
13	4.4338622931049007E-4	0.0	0.010419576388796517	6.650793439657351E-4	0.0
14	4.4338622931049007E-4	0.0	0.010641269503451761	6.650793439657351E-4	0.0
15	4.4338622931049007E-4	0.0	0.011528041962072742	6.650793439657351E-4	0.0
16	4.4338622931049007E-4	0.0	0.012193121306038477	6.650793439657351E-4	0.0
17	4.4338622931049007E-4	0.0	0.012636507535348966	6.650793439657351E-4	0.0
18	4.4338622931049007E-4	0.0	0.013301586879314702	6.650793439657351E-4	0.0
19	4.4338622931049007E-4	0.0	0.013523279993969947	6.650793439657351E-4	0.0
20	4.4338622931049007E-4	0.0	0.014188359337935682	8.867724586209801E-4	0.0
21	4.4338622931049007E-4	0.0	0.014410052452590928	0.0011084655732762252	0.0
22	4.4338622931049007E-4	0.0	0.014853438681901417	0.0011084655732762252	0.0
23	4.4338622931049007E-4	0.0	0.015075131796556663	0.0013301586879314702	0.0
24	4.4338622931049007E-4	0.0	0.015296824911211907	0.0013301586879314702	0.0
25	4.4338622931049007E-4	0.0	0.015296824911211907	0.0015518518025867153	0.0
26	4.4338622931049007E-4	0.0	0.015296824911211907	0.0017735449172419603	0.0
27	4.4338622931049007E-4	0.0	0.015296824911211907	0.0026603173758629403	0.0
28	6.650793439657351E-4	0.0	0.015296824911211907	0.007759259012933576	0.0
29	6.650793439657351E-4	0.0	0.015296824911211907	0.01928730097500632	0.0
30	6.650793439657351E-4	0.0	0.015296824911211907	0.03702275014742592	0.0
31	6.650793439657351E-4	0.0	0.015296824911211907	0.10308729831468894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	260	0.0	29.173077	1
CGTTCGC	45	1.3214716E-4	24.666666	34
GTATCAA	870	0.0	23.603449	1
GTTCTAT	40	0.0019293779	23.125002	1
GTACATG	115	5.456968E-12	22.52174	1
TTCGCCG	60	3.7198282E-5	21.583334	24
CTCGGTA	45	0.0038222594	20.555555	27
GCGAACT	110	1.7462298E-9	20.181818	28
CCGTTCG	55	5.1364396E-4	20.181818	33
AGCTTCG	65	6.890255E-5	19.923077	21
CCGCTCT	65	6.890255E-5	19.923077	28
ATAATAC	75	9.245319E-6	19.733334	3
GTCGCCA	95	1.6704871E-7	19.473684	12
CTCGTCT	95	1.6704871E-7	19.473684	13
CGAACTA	115	3.0358933E-9	19.304348	29
CATGCTA	115	3.0358933E-9	19.304348	4
AACCGTG	60	9.224959E-4	18.5	8
TTTGTCG	50	0.007028419	18.5	36
CGGCAGC	70	1.2171141E-4	18.5	17
ACATTAT	60	9.224959E-4	18.5	1
>>END_MODULE