FastQCFastQC Report
Fri 10 Feb 2017
ERR1633038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393578
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10910.27720045327736814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.20072260136491368No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4800.12195803627235262No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4610.11713053066990534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4570.11611421370096905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC358.8589924E-426.428571
GTATCAA6550.024.0076331
GGTATCA2150.023.232561
ATCGTAC400.001928943123.12500225
CCGTACA400.001928943123.12500217
TCGTTTG502.6977283E-422.212
ACGGGTC502.6977283E-422.216
AGCTTCG555.134839E-420.18181821
CGCAATA555.134839E-420.18181828
ACTATCG951.6691229E-719.47368411
TTCGTTT609.22211E-418.511
CGCAGTA500.007026863318.52
TGCTCGC500.007026863318.510
TAGGACC801.6122722E-518.54
CGCCCTA609.22211E-418.516
TAGTACC609.22211E-418.54
CTAGCAC609.22211E-418.53
GTCAACC1258.527422E-917.7615
CTATCGG1054.777485E-717.61904712
ATCGGTC1054.777485E-717.61904714
Produced by FastQC (version 0.11.5)