##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393578
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690419688092323	33.0	33.0	33.0	27.0	33.0
2	31.1945002007226	33.0	33.0	33.0	27.0	33.0
3	31.64048295382364	33.0	33.0	33.0	27.0	33.0
4	31.7747358846277	33.0	33.0	33.0	27.0	33.0
5	31.89066716127426	33.0	33.0	33.0	33.0	33.0
6	34.747315652805796	37.0	37.0	37.0	27.0	37.0
7	34.925943523266035	37.0	37.0	37.0	27.0	37.0
8	35.08381057884333	37.0	37.0	37.0	33.0	37.0
9	35.175060597899275	37.0	37.0	37.0	33.0	37.0
10	35.2199868895111	37.0	37.0	37.0	33.0	37.0
11	35.25737210921342	37.0	37.0	37.0	33.0	37.0
12	35.206792554461884	37.0	37.0	37.0	33.0	37.0
13	35.232591760718336	37.0	37.0	37.0	33.0	37.0
14	35.21147523489626	37.0	37.0	37.0	33.0	37.0
15	35.23193877706579	37.0	37.0	37.0	33.0	37.0
16	35.224146166706475	37.0	37.0	37.0	33.0	37.0
17	35.19468059698459	37.0	37.0	37.0	33.0	37.0
18	35.18847090030439	37.0	37.0	37.0	33.0	37.0
19	35.19898469934803	37.0	37.0	37.0	33.0	37.0
20	35.20147721671435	37.0	37.0	37.0	33.0	37.0
21	35.188069455101655	37.0	37.0	37.0	33.0	37.0
22	35.184901086951	37.0	37.0	37.0	33.0	37.0
23	35.17890227604185	37.0	37.0	37.0	33.0	37.0
24	35.17194304559706	37.0	37.0	37.0	33.0	37.0
25	35.18005325500917	37.0	37.0	37.0	33.0	37.0
26	35.128330343667585	37.0	37.0	37.0	33.0	37.0
27	35.070204635421696	37.0	37.0	37.0	33.0	37.0
28	35.05658090645311	37.0	37.0	37.0	27.0	37.0
29	35.08821631290367	37.0	37.0	37.0	33.0	37.0
30	35.04396841286861	37.0	37.0	37.0	27.0	37.0
31	35.034544613774145	37.0	37.0	37.0	27.0	37.0
32	35.07328661662999	37.0	37.0	37.0	27.0	37.0
33	35.04315535929346	37.0	37.0	37.0	27.0	37.0
34	34.99723815863692	37.0	37.0	37.0	27.0	37.0
35	34.98321044367317	37.0	37.0	37.0	27.0	37.0
36	34.977033777294466	37.0	37.0	37.0	27.0	37.0
37	34.98701909151426	37.0	37.0	37.0	27.0	37.0
38	34.844843969937344	37.0	37.0	37.0	27.0	37.0
39	34.92880699632602	37.0	37.0	37.0	27.0	37.0
40	34.95778219311039	37.0	37.0	37.0	27.0	37.0
41	34.98421151588757	37.0	37.0	37.0	27.0	37.0
42	34.89675235912576	37.0	37.0	37.0	27.0	37.0
43	34.67456768416934	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	25.0
17	17.0
18	20.0
19	11.0
20	29.0
21	73.0
22	251.0
23	542.0
24	1148.0
25	2087.0
26	3284.0
27	5100.0
28	7150.0
29	9819.0
30	12424.0
31	15975.0
32	20154.0
33	25960.0
34	36831.0
35	66557.0
36	186111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82469040444334	20.589819552922165	13.615344353597001	24.970145689037494
2	15.916794129753187	22.75914812311664	38.40509378064831	22.91896396648187
3	17.711101738410175	27.053595475356857	29.99888205133417	25.2364207348988
4	12.548465615456147	17.970262565488927	39.18537113354913	30.295900685505796
5	13.736031993658182	37.23048544380021	35.48623144586334	13.547251116678268
6	29.539506781374975	39.867573898947605	17.38791294228844	13.205006377388981
7	26.690770317446606	31.95325958259862	23.81103618596568	17.544933913989095
8	24.215530339602314	36.467485479371305	20.452362682873535	18.864621498152843
9	25.717138661205656	15.876395530237971	20.41272632108502	37.99373948747135
10	14.978479488182774	28.24141593280112	33.93025016642191	22.8498544125942
11	34.20033640091672	23.84838583457409	24.036658553069532	17.914619211439664
12	23.130611975262845	25.722728404534806	30.26134590856196	20.885313711640386
13	27.788138564655547	22.333311313132338	26.036008110209412	23.842542012002703
14	21.94482415175645	20.992026993378694	27.508397318955836	29.55475153590902
15	23.847115438362916	28.82783082387735	24.271427772893812	23.053625964865923
16	23.059469787437305	28.561047619531582	24.94168881390728	23.437793779123833
17	22.454761190920223	27.653222487029254	26.352844925275292	23.539171396775227
18	23.167961623871253	27.450975410210937	27.425821565229764	21.955241400688045
19	24.83599184913791	26.29567709577263	26.91156517894801	21.95676587614145
20	24.198761109614868	26.601334424180212	27.187495236014207	22.012409230190713
21	23.073952304244646	27.15217822134367	27.24136003536783	22.53250943904385
22	22.774646956892916	26.97838801965557	27.18952786995208	23.057437153499432
23	22.84477282774952	26.900893850774178	27.63619917779957	22.61813414367673
24	23.101392862405927	27.506872843502432	26.17270274253134	23.219031551560303
25	23.115875379213268	27.098059342747817	27.1382038630208	22.647861415018117
26	23.137472114803163	27.224082646895916	27.212903160237616	22.425542078063305
27	23.103933654828268	27.036318086884936	26.59549060160883	23.264257656677962
28	22.68114579575078	26.864052360650238	27.61053717433393	22.844264669265048
29	23.994481398858674	26.92299874484854	26.53908500983287	22.543434846459913
30	23.439572333819473	26.589646779037444	27.223066329926976	22.747714557216103
31	23.15856069190859	26.664600155496498	27.260161899293152	22.91667725330176
32	22.425033919578837	26.43160949036786	27.47180990807413	23.671546681979176
33	22.440532753355118	26.716686400154483	27.876812220195234	22.96596862629517
34	23.07547677969805	26.45041135429318	27.51068403213594	22.963427833872828
35	23.068108481673264	27.229164231740594	26.995411328885254	22.70731595770089
36	23.650966263358217	26.847029051420556	26.742602482862353	22.75940220235887
37	23.018308950195387	26.432371728094555	26.923252824090778	23.626066497619277
38	23.16465859372221	26.760133950576503	26.98219920828908	23.093008247412204
39	22.748476794942807	26.580245847074785	27.457073312024555	23.214204045957853
40	22.83918308442037	27.138712021505267	27.549304076955522	22.472800817118845
41	22.852141125774306	26.217166609922305	27.354933456646457	23.575758807656932
42	22.577227385677045	27.102886848350266	27.414133920086996	22.905751845885693
43	23.25129961532403	25.937933522707063	27.36560478482029	23.44516207714862
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	13.0
3	53.0
4	93.0
5	93.0
6	115.0
7	137.0
8	146.5
9	156.0
10	196.5
11	237.0
12	237.0
13	357.0
14	477.0
15	967.0
16	1457.0
17	1731.0
18	2005.0
19	2005.0
20	1967.0
21	1929.0
22	2314.0
23	2699.0
24	3739.0
25	4779.0
26	4779.0
27	6354.5
28	7930.0
29	9885.0
30	11840.0
31	14195.5
32	16551.0
33	16551.0
34	18955.0
35	21359.0
36	23489.0
37	25619.0
38	27495.5
39	29372.0
40	29372.0
41	30786.5
42	32201.0
43	32528.5
44	32856.0
45	31668.0
46	30480.0
47	30480.0
48	29421.0
49	28362.0
50	28247.5
51	28133.0
52	26584.5
53	25036.0
54	25036.0
55	24172.0
56	23308.0
57	20565.5
58	17823.0
59	16385.0
60	14947.0
61	14947.0
62	13182.0
63	11417.0
64	9580.5
65	7744.0
66	6574.5
67	5405.0
68	5405.0
69	4445.5
70	3486.0
71	2945.5
72	2405.0
73	1910.5
74	1416.0
75	1416.0
76	1102.0
77	788.0
78	611.5
79	435.0
80	352.0
81	269.0
82	269.0
83	225.5
84	182.0
85	149.5
86	117.0
87	92.0
88	67.0
89	67.0
90	52.0
91	37.0
92	21.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	393578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.26460313658819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.75932167781727	62.171437938233694
2	5.962223521099109	8.259420920011221
3	1.5713317433487113	3.2651300879691516
4	0.734163281441927	2.034061133061215
5	0.3891066042757277	1.3475657261491873
6	0.26373429076093935	1.096047058983961
7	0.20229449797187918	0.9808293683116288
8	0.13791806877201757	0.7642272239086778
9	0.11828216474418064	0.7373490479227994
>10	0.7389726015713879	9.900207659879324
>50	0.08386114892033111	4.041973167315889
>100	0.038051534528428285	4.920429351010988
>500	3.6943237406241055E-4	0.20214983552439966
>1k	3.6943237406241055E-4	0.2791714817178734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.27720045327736814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.20072260136491368	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	480	0.12195803627235262	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	461	0.11713053066990534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.11611421370096905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.54079242234068E-4	0.0	0.0
2	0.0	0.0	2.54079242234068E-4	0.0	0.0
3	0.0	0.0	5.08158484468136E-4	0.0	0.0
4	0.0	0.0	5.08158484468136E-4	0.0	0.0
5	0.0	0.0	7.622377267022039E-4	0.0	0.0
6	0.0	0.0	0.001016316968936272	0.0	0.0
7	0.0	0.0	0.0030489509068088155	0.0	0.0
8	0.0	0.0	0.0035571093912769515	0.0	0.0
9	0.0	0.0	0.005589743329149495	5.08158484468136E-4	0.0
10	2.54079242234068E-4	0.0	0.006097901813617631	5.08158484468136E-4	0.0
11	2.54079242234068E-4	0.0	0.008130535751490175	5.08158484468136E-4	0.0
12	2.54079242234068E-4	0.0	0.008130535751490175	5.08158484468136E-4	0.0
13	2.54079242234068E-4	0.0	0.008384614993724242	5.08158484468136E-4	0.0
14	2.54079242234068E-4	0.0	0.00889277347819238	5.08158484468136E-4	0.0
15	2.54079242234068E-4	0.0	0.009146852720426446	5.08158484468136E-4	0.0
16	2.54079242234068E-4	0.0	0.00990909044712865	5.08158484468136E-4	0.0
17	2.54079242234068E-4	0.0	0.00990909044712865	5.08158484468136E-4	0.0
18	2.54079242234068E-4	0.0	0.010417248931596786	5.08158484468136E-4	0.0
19	2.54079242234068E-4	0.0	0.010417248931596786	5.08158484468136E-4	0.0
20	2.54079242234068E-4	0.0	0.010671328173830855	5.08158484468136E-4	0.0
21	2.54079242234068E-4	0.0	0.010925407416064922	5.08158484468136E-4	0.0
22	2.54079242234068E-4	0.0	0.010925407416064922	5.08158484468136E-4	0.0
23	2.54079242234068E-4	0.0	0.010925407416064922	5.08158484468136E-4	0.0
24	2.54079242234068E-4	0.0	0.010925407416064922	0.0012703962111703397	0.0
25	2.54079242234068E-4	0.0	0.010925407416064922	0.0017785546956384758	0.0
26	2.54079242234068E-4	0.0	0.010925407416064922	0.002032633937872544	2.54079242234068E-4
27	2.54079242234068E-4	0.0	0.010925407416064922	0.0033030301490428837	2.54079242234068E-4
28	2.54079242234068E-4	0.0	0.010925407416064922	0.006860139540319835	2.54079242234068E-4
29	2.54079242234068E-4	0.0	0.010925407416064922	0.01778554695638476	2.54079242234068E-4
30	2.54079242234068E-4	0.0	0.010925407416064922	0.03379253921713104	2.54079242234068E-4
31	2.54079242234068E-4	0.0	0.010925407416064922	0.07749416888139073	2.54079242234068E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTAAC	35	8.8589924E-4	26.42857	1
GTATCAA	655	0.0	24.007633	1
GGTATCA	215	0.0	23.23256	1
ATCGTAC	40	0.0019289431	23.125002	25
CCGTACA	40	0.0019289431	23.125002	17
TCGTTTG	50	2.6977283E-4	22.2	12
ACGGGTC	50	2.6977283E-4	22.2	16
AGCTTCG	55	5.134839E-4	20.181818	21
CGCAATA	55	5.134839E-4	20.181818	28
ACTATCG	95	1.6691229E-7	19.473684	11
TTCGTTT	60	9.22211E-4	18.5	11
CGCAGTA	50	0.0070268633	18.5	2
TGCTCGC	50	0.0070268633	18.5	10
TAGGACC	80	1.6122722E-5	18.5	4
CGCCCTA	60	9.22211E-4	18.5	16
TAGTACC	60	9.22211E-4	18.5	4
CTAGCAC	60	9.22211E-4	18.5	3
GTCAACC	125	8.527422E-9	17.76	15
CTATCGG	105	4.777485E-7	17.619047	12
ATCGGTC	105	4.777485E-7	17.619047	14
>>END_MODULE