FastQCFastQC Report
Fri 10 Feb 2017
ERR1633037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500894
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31380.6264798540210105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.4527904107455869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11560.23078735221424093No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6320.12617440017249157No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5670.11319760268639673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.10601045330948264No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5260.10501223811824457No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5150.10281616469752083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCCG250.005493419229.613
CGTTAGA454.0023224E-628.77777725
GGTATCA5500.027.9181821
ATCTCGC451.3217561E-424.66666611
ACGGGTC1450.024.24137916
CGGGTCA851.9699655E-923.94117517
AACGTTA551.8998811E-523.54545423
TCTCGCC551.8998811E-523.54545412
GTATCAA14900.023.218121
ACGAAAG400.00192967423.12500219
GCCGGCA1600.021.96875215
CGGGTCC603.7208833E-521.58333417
ACGTTAG603.7208833E-521.58333424
GACCATA603.7208833E-521.58333411
TTAGACT450.003822839420.5555554
CGGGCAT450.003822839420.55555517
ATTATAC555.137527E-420.1818183
CCGGCAG1750.019.0285716
TACGGGT3150.018.79365215
CTGTCTA500.00702947618.59