##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500894
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745285429651783	33.0	33.0	33.0	27.0	33.0
2	31.23053580198605	33.0	33.0	33.0	27.0	33.0
3	31.681421618146754	33.0	33.0	33.0	27.0	33.0
4	31.798885592560502	33.0	33.0	33.0	27.0	33.0
5	31.92528558936621	33.0	33.0	33.0	33.0	33.0
6	34.757527540757124	37.0	37.0	37.0	27.0	37.0
7	34.92267226199555	37.0	37.0	37.0	27.0	37.0
8	35.118098839275376	37.0	37.0	37.0	33.0	37.0
9	35.21293527173414	37.0	37.0	37.0	33.0	37.0
10	35.23469037361198	37.0	37.0	37.0	33.0	37.0
11	35.27490047794544	37.0	37.0	37.0	33.0	37.0
12	35.222777673519744	37.0	37.0	37.0	33.0	37.0
13	35.25135457801451	37.0	37.0	37.0	33.0	37.0
14	35.23007063370693	37.0	37.0	37.0	33.0	37.0
15	35.25907876716431	37.0	37.0	37.0	33.0	37.0
16	35.25390202318255	37.0	37.0	37.0	33.0	37.0
17	35.22494180405435	37.0	37.0	37.0	33.0	37.0
18	35.230412023302335	37.0	37.0	37.0	33.0	37.0
19	35.239062556149605	37.0	37.0	37.0	33.0	37.0
20	35.23847760204754	37.0	37.0	37.0	33.0	37.0
21	35.236674825412166	37.0	37.0	37.0	33.0	37.0
22	35.24030832870827	37.0	37.0	37.0	33.0	37.0
23	35.22214680151888	37.0	37.0	37.0	33.0	37.0
24	35.221639708201735	37.0	37.0	37.0	33.0	37.0
25	35.22113660774535	37.0	37.0	37.0	33.0	37.0
26	35.182228176021276	37.0	37.0	37.0	33.0	37.0
27	35.121570631710505	37.0	37.0	37.0	33.0	37.0
28	35.0826761749991	37.0	37.0	37.0	33.0	37.0
29	35.13334757453673	37.0	37.0	37.0	33.0	37.0
30	35.09802672820996	37.0	37.0	37.0	33.0	37.0
31	35.08622183535838	37.0	37.0	37.0	33.0	37.0
32	35.105882681765	37.0	37.0	37.0	33.0	37.0
33	35.07767711332138	37.0	37.0	37.0	33.0	37.0
34	35.008744365075245	37.0	37.0	37.0	27.0	37.0
35	35.01396902338619	37.0	37.0	37.0	27.0	37.0
36	34.9947254309295	37.0	37.0	37.0	27.0	37.0
37	34.99564977819659	37.0	37.0	37.0	27.0	37.0
38	34.84264135725323	37.0	37.0	37.0	27.0	37.0
39	34.89886283325414	37.0	37.0	37.0	27.0	37.0
40	34.920366384903794	37.0	37.0	37.0	27.0	37.0
41	34.92847588511741	37.0	37.0	37.0	27.0	37.0
42	34.84611314968836	37.0	37.0	37.0	27.0	37.0
43	34.61136687602566	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	16.0
16	21.0
17	25.0
18	19.0
19	13.0
20	40.0
21	110.0
22	264.0
23	655.0
24	1407.0
25	2541.0
26	4103.0
27	6342.0
28	8816.0
29	11937.0
30	15822.0
31	20162.0
32	25584.0
33	33348.0
34	47041.0
35	84481.0
36	238144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69504925193754	20.10205752115218	14.071839550883022	24.131053676027264
2	15.535223021238027	23.9521735137574	38.79104161758775	21.72156184741682
3	17.922554472602986	27.28741011072203	31.362723450470558	23.427311966204424
4	12.612049655216474	18.321640906059965	39.512751200852875	29.553558237870686
5	13.78575107707419	37.14618262546567	34.94052633890604	14.127539958554106
6	28.095165843471868	40.45426776922862	17.711132495098763	13.739433892200745
7	25.28399222190723	33.30045877970189	23.389978718052124	18.025570280338755
8	24.073157194935455	35.88723362627621	21.01702156544099	19.022587613347337
9	26.39540501583169	15.601903796012728	20.012417796979	37.99027339117658
10	14.871409919064712	28.419785423662493	33.678383051104625	23.030421606168172
11	33.358754546870195	24.46825076762748	24.034626088553665	18.138368596948656
12	22.74712813489481	26.035648260909493	30.586710960802087	20.63051264339361
13	28.572312704883668	22.662679129716068	26.56709802872464	22.197910136675624
14	22.3839774483224	21.633519267549623	28.394430757805043	27.588072526322932
15	23.904059541539727	28.513817294677118	25.317731895371075	22.26439126841208
16	22.018830331367514	29.031092406776683	25.59383821726753	23.356239044588275
17	22.04678035672218	27.51839710597452	26.56370409707443	23.87111844022887
18	23.302734710337916	26.57708018063702	28.10215334981054	22.018031759214523
19	25.15282674577855	26.094343314154294	28.224734175294575	20.528095764772587
20	24.864741841587243	25.74297156683849	27.953818572392564	21.438468019181702
21	22.87150574772307	27.208151824537723	27.636585784617107	22.2837566431221
22	22.704604167748066	26.911881555778265	27.62121327067204	22.76230100580163
23	22.302123802640878	27.126098535817956	28.254880274069965	22.3168973874712
24	22.83397285653252	26.770134998622463	27.996342539539302	22.39954960530571
25	22.859726808466462	26.75356462644791	28.484669411092966	21.90203915399266
26	22.768290296949058	26.86556437090482	27.81925916461367	22.546886167532453
27	22.891070765471337	26.78091572268783	27.941839990097705	22.386173521743125
28	22.23903660255463	27.03126809265034	28.054039377592865	22.67565592720216
29	22.95116331998387	27.086968500321422	27.610432546606667	22.351435633088037
30	22.780668165320407	27.054426685087062	28.04984687378966	22.115058275802866
31	22.942778312377467	26.938034793788706	27.822852739302128	22.296334154531696
32	21.914017736287516	26.800081454359603	28.25008884115202	23.035811968200857
33	22.16796368093848	26.627989155390164	28.468099038918414	22.73594812475294
34	22.16616689359425	26.62978594273439	28.157254828366877	23.046792335304474
35	22.283157714007356	27.204158963772773	28.045055440871717	22.467627881348147
36	22.58721406125847	26.886926175997317	28.198980223360632	22.326879539383583
37	23.10748381893175	26.54314086413493	27.855793840612986	22.49358147632034
38	22.91582650221404	26.895311183603717	27.56850750857467	22.620354805607573
39	22.40893282810335	26.597443770538277	28.050445802904406	22.943177598453964
40	22.3168973874712	27.04125024456272	28.265261712058837	22.37659065590724
41	22.49018754467013	26.47206794251878	28.331143914680553	22.706600598130542
42	22.380982802748687	26.767539639125243	28.097960846007343	22.75351671211873
43	22.384177091360648	26.39340858544922	28.198381294245888	23.024033028944245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	28.0
2	39.0
3	162.5
4	286.0
5	286.0
6	394.0
7	502.0
8	468.5
9	435.0
10	637.0
11	839.0
12	839.0
13	1187.5
14	1536.0
15	2852.0
16	4168.0
17	4707.0
18	5246.0
19	5246.0
20	4768.0
21	4290.0
22	4658.5
23	5027.0
24	6498.0
25	7969.0
26	7969.0
27	9862.5
28	11756.0
29	14632.5
30	17509.0
31	19679.0
32	21849.0
33	21849.0
34	23731.0
35	25613.0
36	27576.5
37	29540.0
38	31116.5
39	32693.0
40	32693.0
41	34449.5
42	36206.0
43	35638.0
44	35070.0
45	36036.5
46	37003.0
47	37003.0
48	37078.0
49	37153.0
50	37528.5
51	37904.0
52	37940.0
53	37976.0
54	37976.0
55	33892.5
56	29809.0
57	26981.5
58	24154.0
59	21297.0
60	18440.0
61	18440.0
62	15833.0
63	13226.0
64	10748.5
65	8271.0
66	7255.5
67	6240.0
68	6240.0
69	5105.0
70	3970.0
71	3297.5
72	2625.0
73	2209.5
74	1794.0
75	1794.0
76	1278.0
77	762.0
78	573.0
79	384.0
80	304.5
81	225.0
82	225.0
83	182.5
84	140.0
85	126.5
86	113.0
87	93.0
88	73.0
89	73.0
90	52.5
91	32.0
92	20.5
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	500894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.69346886224976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.33842916699848	59.3464478354677
2	5.551781441647669	7.294315625337944
3	1.3375268923981827	2.636003437745451
4	0.6330596960581456	1.6635154972376436
5	0.3896698060858183	1.2799380636328803
6	0.2650488357701149	1.0447186463783755
7	0.18598976928966654	0.8552819182269409
8	0.14262380040172046	0.7495561752564918
9	0.10342947550045153	0.6115176925404965
>10	0.8902721697486914	11.955283556290006
>50	0.10586878411574618	4.82500356169564
>100	0.05385232885551407	5.862407415551585
>500	0.0015298957061225576	0.5569940321787565
>1k	9.179374236735346E-4	1.3190165424601228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3138	0.6264798540210105	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2268	0.4527904107455869	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1156	0.23078735221424093	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	632	0.12617440017249157	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	567	0.11319760268639673	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.10601045330948264	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	526	0.10501223811824457	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	515	0.10281616469752083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.9964303824761327E-4	0.0	0.0	0.0	0.0
3	1.9964303824761327E-4	0.0	0.0	0.0	0.0
4	1.9964303824761327E-4	0.0	1.9964303824761327E-4	0.0	0.0
5	5.989291147428398E-4	0.0	7.985721529904531E-4	0.0	0.0
6	5.989291147428398E-4	0.0	0.0031942886119618124	0.0	0.0
7	5.989291147428398E-4	0.0	0.009582865835885437	1.9964303824761327E-4	0.0
8	5.989291147428398E-4	0.0	0.011379653180113957	1.9964303824761327E-4	0.0
9	5.989291147428398E-4	0.0	0.014773584830323382	3.9928607649522655E-4	0.0
10	0.0017967873442285194	0.0	0.016171086098056675	3.9928607649522655E-4	0.0
11	0.0017967873442285194	0.0	0.024156807627961206	5.989291147428398E-4	0.0
12	0.0017967873442285194	0.0	0.02635288104868495	7.985721529904531E-4	0.0
13	0.0017967873442285194	0.0	0.028149668392913472	7.985721529904531E-4	0.0
14	0.0017967873442285194	0.0	0.030944670928380057	7.985721529904531E-4	0.0
15	0.0017967873442285194	0.0	0.0345382456168371	7.985721529904531E-4	0.0
16	0.0017967873442285194	0.0	0.038131820305294134	7.985721529904531E-4	0.0
17	0.0017967873442285194	0.0	0.03853110638178936	9.982151912380664E-4	0.0
18	0.0017967873442285194	0.0	0.03952932157302743	9.982151912380664E-4	0.0
19	0.0017967873442285194	0.0	0.039928607649522656	9.982151912380664E-4	0.0
20	0.0017967873442285194	0.0	0.04072717980251311	9.982151912380664E-4	0.0
21	0.001996430382476133	0.0	0.042124681070246396	0.0013975012677332928	0.0
22	0.001996430382476133	0.0	0.04272361018498924	0.0017967873442285194	0.0
23	0.001996430382476133	0.0	0.042923253223236854	0.0027950025354665856	0.0
24	0.001996430382476133	0.0	0.04332253929973208	0.0031942886119618124	0.0
25	0.001996430382476133	0.0	0.04332253929973208	0.0033939316502094256	0.0
26	0.001996430382476133	0.0	0.04332253929973208	0.003793217726704652	0.0
27	0.001996430382476133	0.0	0.04332253929973208	0.005590005070933171	0.0
28	0.001996430382476133	0.0	0.043721825376227305	0.016171086098056675	0.0
29	0.001996430382476133	0.0	0.04412111145272253	0.04192503803199878	0.0
30	0.001996430382476133	0.0	0.04412111145272253	0.07207113680738839	0.0
31	0.001996430382476133	0.0	0.04412111145272253	0.16630265086026186	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATCCG	25	0.0054934192	29.6	13
CGTTAGA	45	4.0023224E-6	28.777777	25
GGTATCA	550	0.0	27.918182	1
ATCTCGC	45	1.3217561E-4	24.666666	11
ACGGGTC	145	0.0	24.241379	16
CGGGTCA	85	1.9699655E-9	23.941175	17
AACGTTA	55	1.8998811E-5	23.545454	23
TCTCGCC	55	1.8998811E-5	23.545454	12
GTATCAA	1490	0.0	23.21812	1
ACGAAAG	40	0.001929674	23.125002	19
GCCGGCA	160	0.0	21.968752	15
CGGGTCC	60	3.7208833E-5	21.583334	17
ACGTTAG	60	3.7208833E-5	21.583334	24
GACCATA	60	3.7208833E-5	21.583334	11
TTAGACT	45	0.0038228394	20.555555	4
CGGGCAT	45	0.0038228394	20.555555	17
ATTATAC	55	5.137527E-4	20.181818	3
CCGGCAG	175	0.0	19.02857	16
TACGGGT	315	0.0	18.793652	15
CTGTCTA	50	0.007029476	18.5	9
>>END_MODULE