FastQCFastQC Report
Fri 10 Feb 2017
ERR1633036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326387
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89892.754092534322752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70222.1514337274462525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35061.0741849399639078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.5361733157264229No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG17210.5272881579229565No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14690.4500792004583516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13830.4237301117997959No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13220.405040641937332No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11400.3492786171017841No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11370.3483594628462531No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11010.3373296117798809No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.32017206567663536No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9490.29075912949964305No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8210.2515418812636533No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8060.24694610998599822No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7610.23315879615303306No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7430.22764387061984698No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7410.22703110111615965No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7410.22703110111615965No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6780.2077288617500084No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6700.20527778373525904No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6600.20221393621682235No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6590.20190755146497869No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6590.20190755146497869No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6510.19945647345022932No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6450.19761816493916734No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.19700539543547999No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6330.19394154791704327No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6160.18873300713570088No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6130.18781385288016986No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6120.1875074681283262No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6090.18658831387279518No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6000.18383085110620215No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5930.18168615784329648No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5920.18137977309145278No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5860.17954146458039077No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5830.17862231032485976No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5580.170962691528768No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5560.17034992202508065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.16728607450664396No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5390.16514138124373826No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5210.1596264557105522No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5100.15625622344027185No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5060.15503068443289716No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5020.15380514542552248No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4920.15074129790708576No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.1504349131552421No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4730.14491998762205602No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4720.14461360287021235No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4710.1443072181183687No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4620.14154975535177566No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4560.13971144684071363No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4540.1390986773370263No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4510.13817952308149528No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4440.13603482981858958No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4320.13235821279646554No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4260.13051990428540353No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4200.1286815957743415No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4180.12806882627065416No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4180.12806882627065416No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4150.12714967201512317No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4150.12714967201512317No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4140.12684328726327948No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4140.12684328726327948No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.1256177482559048No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4100.1256177482559048No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4100.1256177482559048No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4060.12439220924853013No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4010.12286028548931177No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3950.12102197697824975No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3880.11887728371534406No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3780.11581343619690736No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3740.11458789718953268No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3720.11397512768584533No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3700.113362358182158No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3700.113362358182158No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3690.11305597343031432No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3680.11274958867847065No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3650.11183043442293963No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG3580.10968574116003395No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3570.10937935640819027No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3560.1090729716563466No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG3480.10662189364159724No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3470.10631550888975357No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3460.1060091241379099No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3450.10570273938606622No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3450.10570273938606622No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3350.10263889186762952No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3310.10141335286025484No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3280.10049419860472383No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3280.10049419860472383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCC250.00549064929.67
CGTTATT453.9967053E-628.77777710
TAGGACC900.028.7777774
TATAGAT405.9273014E-527.755
TTAGGAC950.027.2631593
CTTAGGA950.027.2631592
ATAGATC358.8557357E-426.4285726
GGGCTAG509.065068E-625.90000218
ATTGTTA509.065068E-625.90000214
TTGTTAC509.065068E-625.90000215
GGTATCA12600.025.841271
GAATCAA809.731593E-1025.43751
GACCGTT951.0913936E-1125.315797
GGGTCAA451.320378E-424.66666618
ATGGAGG751.366061E-824.66666628
CTGCCTG751.366061E-824.66666621
TGCCTGA751.366061E-824.66666622
TAGTGAG451.320378E-424.6666667
GTGACAT451.320378E-424.66666616
GCCGTTT1051.8189894E-1224.66666626
Produced by FastQC (version 0.11.5)