FastQCFastQC Report
Fri 10 Feb 2017
ERR1633035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346327
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8700.2512076736725695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.1899938497431618No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4150.11982894778634065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA303.5943312E-430.8333341
TAACCGT453.997631E-628.7777797
GGTATCA2250.027.1333331
CGAGTTC400.001928477823.12500214
GACCGTG400.001928477823.1250027
CTCGCTT400.001928477823.12500212
GCCGAGT502.696822E-422.212
AACCGTG603.7166155E-521.5833348
CCGAGTT450.00382049220.55555713
CTCCTCG450.00382049220.5555579
CCGTGCA656.884348E-519.92307710
GTATCAA5850.019.9230771
AATAGCG759.235151E-619.7333346
CCGTCAC500.00702520118.524
CCCGTCA500.00702520118.523
GTTGTAT500.00702520118.51
CTAGTCT500.00702520118.519
TTAACGG500.00702520118.535
CGTCACC500.00702520118.525
AATCGAA500.00702520118.57
Produced by FastQC (version 0.11.5)