##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346327
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692729703430572	33.0	33.0	33.0	27.0	33.0
2	31.19519124988811	33.0	33.0	33.0	27.0	33.0
3	31.629872923566456	33.0	33.0	33.0	27.0	33.0
4	31.77365322368744	33.0	33.0	33.0	27.0	33.0
5	31.87716233501864	33.0	33.0	33.0	33.0	33.0
6	34.79120311151022	37.0	37.0	37.0	27.0	37.0
7	34.95136965931042	37.0	37.0	37.0	27.0	37.0
8	35.107554421110684	37.0	37.0	37.0	33.0	37.0
9	35.20853701848253	37.0	37.0	37.0	33.0	37.0
10	35.23641818281566	37.0	37.0	37.0	33.0	37.0
11	35.25094202877627	37.0	37.0	37.0	33.0	37.0
12	35.223655678015284	37.0	37.0	37.0	33.0	37.0
13	35.240553003375425	37.0	37.0	37.0	33.0	37.0
14	35.2350581964444	37.0	37.0	37.0	33.0	37.0
15	35.240541453597324	37.0	37.0	37.0	33.0	37.0
16	35.23462796721018	37.0	37.0	37.0	33.0	37.0
17	35.20222217730642	37.0	37.0	37.0	33.0	37.0
18	35.21439853086822	37.0	37.0	37.0	33.0	37.0
19	35.21313960505534	37.0	37.0	37.0	33.0	37.0
20	35.19381105140518	37.0	37.0	37.0	33.0	37.0
21	35.226239940865135	37.0	37.0	37.0	33.0	37.0
22	35.217747966517194	37.0	37.0	37.0	33.0	37.0
23	35.20244739797937	37.0	37.0	37.0	33.0	37.0
24	35.196089245135376	37.0	37.0	37.0	33.0	37.0
25	35.19739148261614	37.0	37.0	37.0	33.0	37.0
26	35.17201084524164	37.0	37.0	37.0	33.0	37.0
27	35.09808071562425	37.0	37.0	37.0	33.0	37.0
28	35.07350856271674	37.0	37.0	37.0	33.0	37.0
29	35.11569990211563	37.0	37.0	37.0	33.0	37.0
30	35.077374850935676	37.0	37.0	37.0	33.0	37.0
31	35.046548493187075	37.0	37.0	37.0	27.0	37.0
32	35.07255281857897	37.0	37.0	37.0	27.0	37.0
33	35.080568364580294	37.0	37.0	37.0	33.0	37.0
34	34.999139541531555	37.0	37.0	37.0	27.0	37.0
35	34.98909412202918	37.0	37.0	37.0	27.0	37.0
36	34.99497584652655	37.0	37.0	37.0	27.0	37.0
37	35.01862979207512	37.0	37.0	37.0	27.0	37.0
38	34.872265229104286	37.0	37.0	37.0	27.0	37.0
39	34.946588051177066	37.0	37.0	37.0	27.0	37.0
40	34.973421073147634	37.0	37.0	37.0	27.0	37.0
41	34.98898151169271	37.0	37.0	37.0	27.0	37.0
42	34.918415254946915	37.0	37.0	37.0	27.0	37.0
43	34.70981471268483	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	10.0
17	21.0
18	19.0
19	6.0
20	26.0
21	63.0
22	206.0
23	483.0
24	1012.0
25	1764.0
26	2821.0
27	4442.0
28	6300.0
29	8326.0
30	10804.0
31	13735.0
32	17623.0
33	22885.0
34	32619.0
35	58973.0
36	164183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.375136792684366	21.12454414469562	13.58340527882608	24.91691378379393
2	15.803272629624605	23.17087607954332	38.15902311976831	22.866828171063762
3	17.31600481625747	27.37528405235514	30.54194446289201	24.766766668495382
4	12.419187646357345	18.15913861754931	39.77772452046765	29.643949215625693
5	13.380995417625538	37.61733852688355	35.95763541392961	13.0440306415613
6	29.106018300623397	40.56541938688003	17.592044512844797	12.736517799651775
7	26.0565881378004	32.467581216595875	24.48033217161815	16.995498473985567
8	23.94615493449832	36.774493470044206	20.81558758052361	18.463764014933865
9	25.496423899955822	15.714050593802966	20.864385392995636	37.925140113245575
10	14.582749828918912	28.674634088592573	34.61064254303014	22.131973539458375
11	33.494356489675944	24.10207693884681	24.626148120129244	17.777418451348005
12	22.664418309863223	26.034354814958117	30.812209270429392	20.489017604749268
13	27.34958579608289	22.80186066925189	26.46285158246397	23.385701952201245
14	21.48403098805464	21.237443225622027	27.957392868589515	29.321132917733816
15	23.1007111775865	29.43865190990018	24.619506997721807	22.841129914791512
16	22.85383467070139	28.9203556176677	25.493247710978352	22.73256200065256
17	21.989333779924753	28.17972609701236	26.90520808368969	22.92573203937319
18	22.57663999630407	28.053833515723582	28.005324447704048	21.364202040268303
19	24.275612354797634	26.920800284124542	27.336014806815523	21.4675725542623
20	23.677622593675917	27.021860842498562	27.64121769310507	21.65929887072045
21	22.626015297681093	27.590109925013067	27.684240616527156	22.099634160778685
22	22.43313400341296	27.37355158564016	27.578560146913176	22.614754264033703
23	22.449881181657798	27.347564584915414	28.116202317462974	22.086351915963814
24	22.6979126663529	27.992330947341674	26.537347651208247	22.772408735097176
25	22.807924302754333	27.562101713120835	27.6588311047074	21.971142879417428
26	22.527553439379545	27.783279963733698	27.67009213835479	22.019074458531964
27	22.43400023677045	27.684240616527156	27.165655579842173	22.71610356686022
28	22.336404611826396	27.27249102726614	28.043727459886174	22.347376901021292
29	23.335749161919225	27.288949461058483	27.312049017258254	22.06325235976404
30	23.029680042272187	27.14919714604983	27.64035145974758	22.1807713519304
31	22.742956800942462	27.355360685132833	27.57220776895824	22.32947474496646
32	21.94544462314518	27.325908750978122	27.741123273669103	22.987523352207596
33	22.027736792106882	27.380481452500092	28.078665538638337	22.513116216754685
34	22.932084417328134	26.74726486817372	28.026114048283844	22.294536666214302
35	22.688961588325483	27.683085638717163	27.462484877009302	22.165467895948048
36	23.334016695204244	27.403292264247376	26.993852630606334	22.26883840994205
37	22.521489805877103	27.07470107730555	27.416574509062247	22.9872346077551
38	22.75768276801982	27.357381896300314	27.366044229875236	22.51889110580463
39	22.28616307709188	27.304830405945822	27.881164333130247	22.527842183832043
40	22.4080132360457	27.573651491220723	27.717446228564334	22.30088904416924
41	22.29540289957179	26.901743150259726	27.843627554305613	22.95922639586287
42	22.232167864474903	27.41628576460975	27.71773497301683	22.63381139789852
43	22.75854900137731	26.633210809437323	27.930539634507273	22.677700554678093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	11.0
2	14.0
3	44.0
4	74.0
5	74.0
6	112.5
7	151.0
8	132.0
9	113.0
10	156.0
11	199.0
12	199.0
13	328.5
14	458.0
15	856.5
16	1255.0
17	1496.5
18	1738.0
19	1738.0
20	1807.5
21	1877.0
22	2430.0
23	2983.0
24	4080.0
25	5177.0
26	5177.0
27	6485.5
28	7794.0
29	9756.0
30	11718.0
31	13839.5
32	15961.0
33	15961.0
34	17906.5
35	19852.0
36	21776.0
37	23700.0
38	25508.0
39	27316.0
40	27316.0
41	28314.5
42	29313.0
43	29760.5
44	30208.0
45	28868.5
46	27529.0
47	27529.0
48	26198.5
49	24868.0
50	24581.5
51	24295.0
52	22634.5
53	20974.0
54	20974.0
55	19941.0
56	18908.0
57	16284.5
58	13661.0
59	12610.5
60	11560.0
61	11560.0
62	10030.5
63	8501.0
64	7110.0
65	5719.0
66	4844.0
67	3969.0
68	3969.0
69	3263.5
70	2558.0
71	2064.0
72	1570.0
73	1273.0
74	976.0
75	976.0
76	744.5
77	513.0
78	416.0
79	319.0
80	249.0
81	179.0
82	179.0
83	156.0
84	133.0
85	117.0
86	101.0
87	82.5
88	64.0
89	64.0
90	39.0
91	14.0
92	9.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	346327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.9498895740983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40105853656857	60.74792055367333
2	5.819867474623063	7.90918704473047
3	1.5667060904446668	3.1937251752234705
4	0.8042518110415567	2.185952870001694
5	0.49859066283681147	1.6939590241218911
6	0.31272017568683413	1.27495808433078
7	0.2532708821364105	1.2046809931452491
8	0.18256294434778447	0.9924105527003367
9	0.14399101358591215	0.8805756125542697
>10	0.8977556214095211	11.483426532878575
>50	0.08418381040083953	3.8357820811649317
>100	0.03418631894446035	4.1537367654057755
>500	8.546579736115087E-4	0.44368471006920823
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.2512076736725695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	658	0.1899938497431618	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	415	0.11982894778634065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	2.887444524972064E-4	0.0	0.0
5	0.0	0.0	2.887444524972064E-4	0.0	0.0
6	0.0	0.0	8.662333574916192E-4	0.0	2.887444524972064E-4
7	0.0	0.0	0.0020212111674804446	0.0	2.887444524972064E-4
8	0.0	0.0	0.0020212111674804446	0.0	2.887444524972064E-4
9	0.0	0.0	0.0031761889774692704	2.887444524972064E-4	2.887444524972064E-4
10	0.0	0.0	0.003464933429966477	2.887444524972064E-4	2.887444524972064E-4
11	0.0	0.0	0.005197400144949715	2.887444524972064E-4	2.887444524972064E-4
12	0.0	0.0	0.006063633502441335	2.887444524972064E-4	2.887444524972064E-4
13	0.0	0.0	0.006352377954938541	2.887444524972064E-4	2.887444524972064E-4
14	0.0	0.0	0.006929866859932954	2.887444524972064E-4	2.887444524972064E-4
15	0.0	0.0	0.0075073557649273665	2.887444524972064E-4	2.887444524972064E-4
16	0.0	0.0	0.008084844669921779	2.887444524972064E-4	5.774889049944128E-4
17	0.0	0.0	0.008662333574916192	2.887444524972064E-4	5.774889049944128E-4
18	0.0	0.0	0.009239822479910604	2.887444524972064E-4	5.774889049944128E-4
19	0.0	0.0	0.009239822479910604	2.887444524972064E-4	5.774889049944128E-4
20	0.0	0.0	0.009239822479910604	2.887444524972064E-4	5.774889049944128E-4
21	0.0	0.0	0.009528566932407811	2.887444524972064E-4	5.774889049944128E-4
22	0.0	0.0	0.009528566932407811	8.662333574916192E-4	5.774889049944128E-4
23	2.887444524972064E-4	0.0	0.009528566932407811	0.0011549778099888255	5.774889049944128E-4
24	2.887444524972064E-4	0.0	0.009528566932407811	0.0017324667149832384	5.774889049944128E-4
25	2.887444524972064E-4	0.0	0.009528566932407811	0.0017324667149832384	5.774889049944128E-4
26	2.887444524972064E-4	0.0	0.009528566932407811	0.0017324667149832384	5.774889049944128E-4
27	2.887444524972064E-4	0.0	0.009528566932407811	0.0017324667149832384	5.774889049944128E-4
28	2.887444524972064E-4	0.0	0.009528566932407811	0.00721861131243016	5.774889049944128E-4
29	2.887444524972064E-4	0.0	0.009528566932407811	0.014725967077357526	5.774889049944128E-4
30	2.887444524972064E-4	0.0	0.009528566932407811	0.02829695634472623	5.774889049944128E-4
31	2.887444524972064E-4	0.0	0.009528566932407811	0.0770947688167541	5.774889049944128E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTATA	30	3.5943312E-4	30.833334	1
TAACCGT	45	3.997631E-6	28.777779	7
GGTATCA	225	0.0	27.133333	1
CGAGTTC	40	0.0019284778	23.125002	14
GACCGTG	40	0.0019284778	23.125002	7
CTCGCTT	40	0.0019284778	23.125002	12
GCCGAGT	50	2.696822E-4	22.2	12
AACCGTG	60	3.7166155E-5	21.583334	8
CCGAGTT	45	0.003820492	20.555557	13
CTCCTCG	45	0.003820492	20.555557	9
CCGTGCA	65	6.884348E-5	19.923077	10
GTATCAA	585	0.0	19.923077	1
AATAGCG	75	9.235151E-6	19.733334	6
CCGTCAC	50	0.007025201	18.5	24
CCCGTCA	50	0.007025201	18.5	23
GTTGTAT	50	0.007025201	18.5	1
CTAGTCT	50	0.007025201	18.5	19
TTAACGG	50	0.007025201	18.5	35
CGTCACC	50	0.007025201	18.5	25
AATCGAA	50	0.007025201	18.5	7
>>END_MODULE