FastQCFastQC Report
Fri 10 Feb 2017
ERR1633034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423427
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20980.4954809211505171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.3870797091352228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7260.17145812619412557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTCG200.001840623237.024
GGTATCA2900.030.620691
TTTAACG454.0003997E-628.77777734
TTAACGG454.0003997E-628.77777735
TTCGCCG604.309186E-827.75000224
GTATCAA9300.024.6666661
AACGGCC551.8989813E-523.54545437
CTCGTAT400.001929183723.12500236
GCTTCGC802.7155693E-823.12500222
TGCTCGC753.7292375E-722.210
CGAGTCG855.1612005E-821.76470621
GCCGGCA705.0893723E-621.14285915
CCGCTCT705.0893723E-621.14285928
AGTGCAC450.003821877320.55555510
CTTAGCC450.003821877320.5555553
CTGCTCG1202.3646862E-1020.0416689
GTTTAAC656.8889785E-519.92307733
TAACGGC656.8889785E-519.92307736
GCCGCTC759.243124E-619.73333427
GCCGTCC951.6698687E-719.47368427
Produced by FastQC (version 0.11.5)