##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633034.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423427
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39732232474547	33.0	33.0	33.0	27.0	33.0
2	30.985858247112255	33.0	33.0	33.0	27.0	33.0
3	31.423626740854978	33.0	33.0	33.0	27.0	33.0
4	31.576425216152963	33.0	33.0	33.0	27.0	33.0
5	31.70124484267654	33.0	33.0	33.0	27.0	33.0
6	34.491395210980876	37.0	33.0	37.0	27.0	37.0
7	34.706081567779094	37.0	37.0	37.0	27.0	37.0
8	34.87891891636575	37.0	37.0	37.0	27.0	37.0
9	34.946510260328225	37.0	37.0	37.0	27.0	37.0
10	34.99359275624842	37.0	37.0	37.0	27.0	37.0
11	35.00248448965229	37.0	37.0	37.0	27.0	37.0
12	34.95491548720322	37.0	37.0	37.0	27.0	37.0
13	34.98648645457186	37.0	37.0	37.0	27.0	37.0
14	34.952546719977704	37.0	37.0	37.0	27.0	37.0
15	34.99633230757604	37.0	37.0	37.0	27.0	37.0
16	34.96363245612585	37.0	37.0	37.0	27.0	37.0
17	34.94895932474783	37.0	37.0	37.0	27.0	37.0
18	34.952270403162764	37.0	37.0	37.0	27.0	37.0
19	34.952910419033266	37.0	37.0	37.0	27.0	37.0
20	34.949927614441215	37.0	37.0	37.0	27.0	37.0
21	34.93782399327393	37.0	37.0	37.0	27.0	37.0
22	34.94408481273041	37.0	37.0	37.0	27.0	37.0
23	34.93611413537634	37.0	37.0	37.0	27.0	37.0
24	34.946819640693676	37.0	37.0	37.0	27.0	37.0
25	34.92668157675349	37.0	37.0	37.0	27.0	37.0
26	34.881084578923875	37.0	37.0	37.0	27.0	37.0
27	34.79715039428284	37.0	37.0	37.0	27.0	37.0
28	34.778946548047244	37.0	37.0	37.0	27.0	37.0
29	34.81260760414428	37.0	37.0	37.0	27.0	37.0
30	34.760556128919504	37.0	37.0	37.0	27.0	37.0
31	34.7543094795561	37.0	37.0	37.0	27.0	37.0
32	34.782588261967234	37.0	37.0	37.0	27.0	37.0
33	34.734013182909926	37.0	37.0	37.0	27.0	37.0
34	34.669206262236465	37.0	37.0	37.0	27.0	37.0
35	34.665359081966905	37.0	37.0	37.0	27.0	37.0
36	34.63367947721803	37.0	37.0	37.0	27.0	37.0
37	34.65966034286902	37.0	37.0	37.0	27.0	37.0
38	34.51349583281179	37.0	37.0	37.0	27.0	37.0
39	34.590729925111056	37.0	37.0	37.0	27.0	37.0
40	34.62332586254537	37.0	37.0	37.0	27.0	37.0
41	34.61446010764547	37.0	37.0	37.0	27.0	37.0
42	34.51778937101319	37.0	37.0	37.0	27.0	37.0
43	34.26717238154393	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	27.0
17	22.0
18	17.0
19	31.0
20	55.0
21	144.0
22	384.0
23	898.0
24	1639.0
25	3001.0
26	4718.0
27	6721.0
28	9223.0
29	12221.0
30	15369.0
31	19334.0
32	23826.0
33	30769.0
34	42163.0
35	73199.0
36	179652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53851077045157	20.81374121159019	14.101368122486285	24.546379895471947
2	15.690071724287776	23.79654580364502	37.94821775654363	22.565164715523572
3	17.731273631582305	27.330330848056455	30.74343393312188	24.19496158723936
4	12.625789097058052	18.568017627595783	39.454734818516535	29.351458456829633
5	13.992494574035193	37.222236654724426	34.978166248255306	13.80710252298507
6	28.574465019944405	40.5080450703427	17.733399145543387	13.184090764169504
7	25.756505843982552	32.63112649878256	23.94297009874193	17.669397558492964
8	24.294860743410315	35.879856504190805	21.07210924197087	18.75317351042801
9	25.931270325227253	15.455792852132715	20.399265989178772	38.21367083346126
10	14.826404551433894	28.54494399270713	34.144492439074504	22.484159016784474
11	33.58642694018095	24.136864205636392	24.530320456654866	17.74638839752779
12	22.42842331736049	26.226952934035857	30.783582530164587	20.56104121843907
13	28.490389134372627	22.405278832006463	26.182789477288882	22.921542556332025
14	22.08385388744695	21.41549783079492	28.091737182560394	28.408911099197738
15	23.59367730447043	29.024837811476356	24.604949613510712	22.776535270542503
16	22.401736308737988	28.593594645594163	25.718482760900933	23.286186284766913
17	21.9827738901865	27.59861794358886	27.0150462771623	23.403561889062342
18	23.256429089311734	26.92199599931039	27.87257307635082	21.949001835027055
19	24.484503822382607	26.585928625241184	27.662619530639283	21.266948021736923
20	24.067430749574307	26.72030834122529	27.43023000422741	21.78203090497299
21	22.872655735227088	27.227125336834924	27.648921774001185	22.251297153936804
22	22.73756751458929	26.78100357322514	27.863362515852792	22.618066396332782
23	22.68797218883066	26.94891917615079	28.224463721019205	22.138644913999343
24	22.642864059212094	27.19736814137974	27.39291542579949	22.766852373608675
25	22.956495452580963	26.97844020338807	28.298620541439256	21.76644380259171
26	22.73780368280719	27.074324499854757	27.925002420724233	22.262869396613823
27	22.641919386340504	26.970646652197427	27.83407765683341	22.55335630462866
28	22.633417330496165	27.067475621535706	28.043086529673356	22.256020518294772
29	22.935712649405918	27.0150462771623	27.555635327931377	22.493605745500407
30	22.992629189919395	27.194061786329165	27.756378313144886	22.05693071060655
31	23.319249835272668	26.735423107170774	27.671593922919417	22.273733134637137
32	22.261216219088535	27.023548333006637	27.9030387764597	22.812196671445136
33	22.217525098777358	26.99568048329464	28.137789985050553	22.64900443287745
34	22.77157573796664	26.450604236385495	27.83242447930812	22.945395546339746
35	22.49289724084671	27.12651767601027	28.10354559345531	22.277039489687713
36	23.03088844121891	26.800133198874892	27.381579351340374	22.78739900856582
37	22.909261809001315	26.585692457023292	27.873517749222415	22.63152798475298
38	22.70828265556991	27.055667210640795	27.57193093496636	22.664119198822938
39	22.27751182612351	26.76706964836914	28.098349892661545	22.857068632845802
40	22.36938126288593	26.757150583217417	28.55132053459038	22.32214761930628
41	22.447552943010248	26.473276385303723	28.0374184924438	23.04175217924223
42	22.165568090839745	27.133602722547217	28.012384661346584	22.68844452526646
43	22.558079668986625	26.52877591650981	28.277129233610516	22.636015180893047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	21.0
2	29.0
3	135.5
4	242.0
5	242.0
6	288.5
7	335.0
8	316.0
9	297.0
10	432.0
11	567.0
12	567.0
13	872.5
14	1178.0
15	2071.5
16	2965.0
17	3339.5
18	3714.0
19	3714.0
20	3429.0
21	3144.0
22	3625.0
23	4106.0
24	5463.5
25	6821.0
26	6821.0
27	8313.5
28	9806.0
29	11897.5
30	13989.0
31	15971.5
32	17954.0
33	17954.0
34	20280.0
35	22606.0
36	24272.5
37	25939.0
38	27258.5
39	28578.0
40	28578.0
41	30292.5
42	32007.0
43	32320.0
44	32633.0
45	33002.5
46	33372.0
47	33372.0
48	32874.5
49	32377.0
50	31589.0
51	30801.0
52	29510.5
53	28220.0
54	28220.0
55	26226.0
56	24232.0
57	21319.0
58	18406.0
59	17276.5
60	16147.0
61	16147.0
62	13769.5
63	11392.0
64	9436.0
65	7480.0
66	6454.5
67	5429.0
68	5429.0
69	4512.5
70	3596.0
71	2876.0
72	2156.0
73	1786.5
74	1417.0
75	1417.0
76	1040.5
77	664.0
78	499.5
79	335.0
80	271.5
81	208.0
82	208.0
83	170.0
84	132.0
85	102.0
86	72.0
87	60.5
88	49.0
89	49.0
90	33.0
91	17.0
92	9.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.61166978263059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98317604266582	60.16301114806407
2	6.103251321740304	8.253020259318184
3	1.6819115725594727	3.4115054954242803
4	0.8048344570450764	2.1766480615765795
5	0.4814808102464911	1.6276860774529587
6	0.31362628157078287	1.272287795083086
7	0.2317085219088931	1.0966340048387855
8	0.17926008531017124	0.969605895455799
9	0.13273953470181857	0.8077267428623454
>10	0.9808688530856129	12.745979187932571
>50	0.08009342417444797	3.746735572738887
>100	0.025995234147279198	2.6691482946614853
>500	3.5128694793620536E-4	0.17243296511160688
>1k	7.025738958724107E-4	0.887578499479442
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2098	0.4954809211505171	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1639	0.3870797091352228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.17145812619412557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.001653177525287712	0.0	0.0
2	0.0	0.0	0.001653177525287712	0.0	0.0
3	0.0	0.0	0.0023616821789824455	0.0	0.0
4	0.0	0.0	0.0025978503968806903	0.0	0.0
5	0.0	0.0	0.003306355050575424	0.0	0.0
6	0.0	0.0	0.006848878319049092	0.0	0.0
7	0.0	0.0	0.014406261291792918	2.3616821789824457E-4	0.0
8	0.0	0.0	0.015823270599182385	2.3616821789824457E-4	0.0
9	0.0	0.0	0.018184952778164832	7.085046536947336E-4	0.0
10	2.3616821789824457E-4	0.0	0.021018971392943765	7.085046536947336E-4	0.0
11	2.3616821789824457E-4	0.0	0.03377205515944897	7.085046536947336E-4	0.0
12	2.3616821789824457E-4	0.0	0.03613373733843142	7.085046536947336E-4	0.0
13	2.3616821789824457E-4	0.0	0.04038476526059982	7.085046536947336E-4	0.0
14	2.3616821789824457E-4	0.0	0.042746447439582266	7.085046536947336E-4	0.0
15	2.3616821789824457E-4	0.0	0.046761307143852425	7.085046536947336E-4	0.0
16	2.3616821789824457E-4	0.0	0.050776166848122584	7.085046536947336E-4	0.0
17	2.3616821789824457E-4	0.0	0.054082521898698006	7.085046536947336E-4	0.0
18	2.3616821789824457E-4	0.0	0.05644420407768045	7.085046536947336E-4	0.0
19	2.3616821789824457E-4	0.0	0.058805886256662894	7.085046536947336E-4	0.0
20	2.3616821789824457E-4	0.0	0.05951439091035763	7.085046536947336E-4	0.0
21	2.3616821789824457E-4	0.0	0.06211224130723832	9.446728715929783E-4	0.0
22	2.3616821789824457E-4	0.0	0.0625845777430348	0.0011808410894912228	0.0
23	2.3616821789824457E-4	0.0	0.06329308239672954	0.0018893457431859565	0.0
24	2.3616821789824457E-4	0.0	0.06329308239672954	0.0023616821789824455	0.0
25	2.3616821789824457E-4	0.0	0.06376541883252604	0.0023616821789824455	0.0
26	2.3616821789824457E-4	0.0	0.06400158705042427	0.0023616821789824455	2.3616821789824457E-4
27	2.3616821789824457E-4	0.0	0.06400158705042427	0.004014859704270158	2.3616821789824457E-4
28	2.3616821789824457E-4	0.0	0.06400158705042427	0.014406261291792918	4.7233643579648913E-4
29	2.3616821789824457E-4	0.0	0.06400158705042427	0.033299718723652484	4.7233643579648913E-4
30	2.3616821789824457E-4	0.0	0.06400158705042427	0.05856971803876465	4.7233643579648913E-4
31	2.3616821789824457E-4	0.0	0.06400158705042427	0.13744990281677832	4.7233643579648913E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCTCG	20	0.0018406232	37.0	24
GGTATCA	290	0.0	30.62069	1
TTTAACG	45	4.0003997E-6	28.777777	34
TTAACGG	45	4.0003997E-6	28.777777	35
TTCGCCG	60	4.309186E-8	27.750002	24
GTATCAA	930	0.0	24.666666	1
AACGGCC	55	1.8989813E-5	23.545454	37
CTCGTAT	40	0.0019291837	23.125002	36
GCTTCGC	80	2.7155693E-8	23.125002	22
TGCTCGC	75	3.7292375E-7	22.2	10
CGAGTCG	85	5.1612005E-8	21.764706	21
GCCGGCA	70	5.0893723E-6	21.142859	15
CCGCTCT	70	5.0893723E-6	21.142859	28
AGTGCAC	45	0.0038218773	20.555555	10
CTTAGCC	45	0.0038218773	20.555555	3
CTGCTCG	120	2.3646862E-10	20.041668	9
GTTTAAC	65	6.8889785E-5	19.923077	33
TAACGGC	65	6.8889785E-5	19.923077	36
GCCGCTC	75	9.243124E-6	19.733334	27
GCCGTCC	95	1.6698687E-7	19.473684	27
>>END_MODULE