FastQCFastQC Report
Fri 10 Feb 2017
ERR1633033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436087
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48211.1055133494004639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.8266699076101787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18830.431794573101239No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10330.23687933829717464No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10140.23252240951920142No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9800.22472580012703885No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9230.21165501379311924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.21050845358838946No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8660.19858422745919965No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8530.1956031709269022No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8260.18941174582136133No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8130.1864306892890639No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7910.18138582438825282No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7830.17955132806068513No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7780.17840476785595535No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7750.17771683173311748No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7710.17679958356933365No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7690.17634095948744172No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7440.1706081584637928No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7350.16854435009527916No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7260.16648054172676552No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7210.16533398152203574No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6540.14997007477865656No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6530.1497407627377106No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6520.14951145069676464No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6430.147447642328251No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6390.14653039416446717No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6320.1449252098778455No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT6240.14309071355027783No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6100.1398803449770344No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6070.13919240885419654No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6040.13850447273135866No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6010.13781653660852078No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5690.13047855129825012No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5360.1229112539470335No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5350.12268194190608755No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC5330.12222331782419563No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5320.12199400578324968No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5280.12107675761946583No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5240.120159509455682No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.11970088537379009No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5210.11947157333284414No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5190.11901294925095221No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5180.11878363721000626No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5160.11832501312811433No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4880.11190427598162753No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4880.11190427598162753No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4880.11190427598162753No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4870.11167496394068156No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4710.10800597128554623No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4680.10731803516270835No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4670.1070887231217624No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4660.10685941108081645No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4640.10640078699892452No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4550.1043369786304109No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4530.103878354548519No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4520.10364904250757302No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4390.10066798597527557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACC200.001840683637.08
CACATAG303.5958484E-430.83333214
GCCACAT303.5958484E-430.83333212
ATAGACC250.00549265129.5999983
GGTATCA8600.027.9651171
CAATCAG405.9318063E-527.756
GATGTCT601.3342287E-624.66666624
CTCTAAC451.321373E-424.6666661
AATCAGC551.8991504E-523.5454547
ATGTCTA400.001929275923.12525
TCTAGTG400.001929275923.12528
CTGGTCA400.001929275923.12531
ATACTGA400.001929275923.1256
ACTAGTG400.001929275923.1258
CTAGTGA400.001929275923.12529
CTCAGTT400.001929275923.12515
GGGATCT400.001929275923.12518
CCACATA400.001929275923.12513
GTATCAA24150.023.0579721
ACGATCG502.698378E-422.19999916
Produced by FastQC (version 0.11.5)