FastQCFastQC Report
Fri 10 Feb 2017
ERR1633032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362409
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50371.389866145708302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38641.0661986871186973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20290.5598646832722145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.25882359433678526No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9120.2516493795683882No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8620.23785281270608621No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7690.21219119834220454No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7660.2113634043304664No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7570.20888002229525207No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7470.20612070892279166No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7320.20198173886410106No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7020.19370379874671986No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6990.19287600473498176No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6960.19204821072324363No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6850.18901296601353718No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6790.18735737799006094No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6720.1854258586293387No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6610.18239061391963224No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6570.1812868885706481No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6360.17549233048848126No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6190.17080149775529857No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5990.16528287101037778No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5940.1639032143241476No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5900.16279948897516341No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5720.1578327249047347No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.15121037281082975No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5460.15065851013633766No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5420.1495547847873535No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5380.14845105943836936No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5220.14403615804243272No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5120.1412768446699723No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5070.13989718798374212No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5050.13934532530925006No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5000.13796566862301984No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4970.13713787461128174No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4960.1368619432740357No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4720.13023959118013073No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4620.12748027780767032No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4570.12610062112144016No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4530.12499689577245597No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4500.12416910176071785No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4480.1236172390862258No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4470.12334130774897974No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4280.11809861234130499No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4250.11727081832956687No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4170.11506336763159855No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT4060.11202812292189211No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT3940.10871694687493963No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC3910.10788915286320151No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3850.10623356483972528No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC3820.10540577082798716No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC3790.10457797681624903No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG3740.10319832013001885No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT3710.10237052611828074No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG3710.10237052611828074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAC200.001840273537.026
AGATCTT250.0054914429.68
ATGATCA250.0054914429.625
CATGATC250.0054914429.624
ATAGATC250.0054914429.66
CTAGTGG250.0054914429.69
GTACTCG250.0054914429.636
GGTATCA7150.028.9790231
TTATAGA358.857633E-426.428574
TATAGAT358.857633E-426.428575
GGTCTTA509.068679E-625.90000229
GTATCAA21500.024.6953491
GAATCAA601.3331883E-624.6666681
CCGCTCT1600.023.12500228
TGTCCCC400.001928650223.12500210
GGCAGCT1650.022.42424418
GCTCTCT1650.022.42424430
CGCTCTC1650.022.42424429
CGGGAGC502.697158E-422.217
GCCGCTC1700.021.76470627
Produced by FastQC (version 0.11.5)