FastQCFastQC Report
Fri 10 Feb 2017
ERR1633031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214009
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4830.22569144288324322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.19391707825371832No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3530.16494633403268089No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3230.1509282319902434No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC2640.12335929797344973No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA2430.11354662654374349No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT2400.11214481633949973No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC2350.1098084659990935No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC2310.10793938572676849No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG2170.10139760477363101No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG2170.10139760477363101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2150.10046306463746851No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG2150.10046306463746851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC303.589783E-430.8333322
GGTATCA1300.029.8846151
TATACCC400.001926083223.1250022
GTATCAA3400.020.676471
GTCGCCC909.388168E-820.55555537
ATAGGCG450.003815794120.55555532
TTACACC450.003815794120.55555524
CTCTAAT555.1243213E-420.1818181
AGGTCGC951.6602462E-719.47368435
GTTTGTC500.00701664418.535
AGATCAC1900.018.537
TGAGTAT701.2133243E-418.53
ACGGGGC500.00701664418.515
TTGAGTA701.2133243E-418.52
GCCTGAA500.00701664418.530
ATATACA609.203374E-418.54
TACTGGT701.2133243E-418.57
GTATACC500.00701664418.51
ACCTTAG609.203374E-418.51
AAAGAGT500.00701664418.56
Produced by FastQC (version 0.11.5)