FastQCFastQC Report
Fri 10 Feb 2017
ERR1633030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325923
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9090.27890023103616496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.19268354795457823No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4570.140217167858666No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4550.13960352598619918No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3890.11935334419479447No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3600.11045553704402573No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3580.10984189517155893No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3550.1089214323628587No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3460.10616004393675807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3460.10616004393675807No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3360.10309183457442402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA250.005490637429.615
CGCAATA250.005490637429.634
AGTACCG754.6020432E-1027.1333315
TAACGGC1000.025.90000236
AACGGCC1000.025.90000237
GGTATCA2150.024.9534871
TACCGCA901.4188117E-1024.6666687
TTAACGG1051.8189894E-1224.66666635
CGCAAGG851.9626896E-923.94117510
TAGTACC851.9626896E-923.9411754
CCGCAAG903.803507E-922.6111139
GTATCAA5900.021.0084741
GTACTAT806.928676E-720.81251
GATATAT450.00382001620.5555571
GTATACG450.00382001620.5555571
TATACAG450.00382001620.5555575
CAAGGGA1001.2812961E-820.3512
GTATTAG1750.020.0857141
TCAACCC1202.3646862E-1020.04166616
GACACAT1403.6379788E-1219.82142826