FastQCFastQC Report
Fri 10 Feb 2017
ERR1633029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197237
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7070.35845201458144266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.281387366467752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3400.17238144972799221No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC2780.14094718536582893No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA2510.12725807024037072No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2480.12573705744865316No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2200.11154093805928907No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2150.10900591673975978No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2130.10799190821194807No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA1990.10089384851726603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG358.843255E-426.42857224
ATACAAT358.843255E-426.4285726
TAATACA509.041325E-625.8999984
GGTATCA1600.024.281251
CTTCGCC400.0019255523.12523
TTAGGCT400.0019255523.1254
TTTAAGC400.0019255523.12522
CCGGCAG400.0019255523.12516
AGCTTCG400.0019255523.12521
CGCTCTC400.0019255523.12529
GCTTCGC400.0019255523.12522
TAGGCTG400.0019255523.1255
GGACTAC502.691119E-422.1999991
GCCGCTC450.003814745520.55555727
ACATAAT450.003814745520.5555571
GTATCAA3650.020.2739721
CGGCAGC555.122358E-420.18181817
TGGCGGC609.199878E-418.515
CTAATAC801.6056325E-518.53
TCACGGT500.0070147418.4999981
Produced by FastQC (version 0.11.5)