##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197237
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.367410779924658	33.0	33.0	33.0	14.0	33.0
2	30.26568037437195	33.0	33.0	33.0	14.0	33.0
3	30.736991538098835	33.0	33.0	33.0	27.0	33.0
4	30.926063568194607	33.0	33.0	33.0	27.0	33.0
5	31.058513362097376	33.0	33.0	33.0	27.0	33.0
6	33.24423916405137	37.0	33.0	37.0	27.0	37.0
7	33.56958380019976	37.0	33.0	37.0	27.0	37.0
8	33.71866333395864	37.0	33.0	37.0	27.0	37.0
9	33.70882745123887	37.0	33.0	37.0	27.0	37.0
10	33.67237891470667	37.0	33.0	37.0	27.0	37.0
11	33.713294158803876	37.0	33.0	37.0	27.0	37.0
12	33.78541551534448	37.0	33.0	37.0	27.0	37.0
13	33.915340428012996	37.0	33.0	37.0	27.0	37.0
14	33.851670832551704	37.0	33.0	37.0	27.0	37.0
15	33.944924126811905	37.0	33.0	37.0	27.0	37.0
16	33.897478667795596	37.0	33.0	37.0	27.0	37.0
17	33.906660515014934	37.0	33.0	37.0	27.0	37.0
18	33.90193016523269	37.0	33.0	37.0	27.0	37.0
19	33.90434857557152	37.0	33.0	37.0	27.0	37.0
20	33.8883018906189	37.0	33.0	37.0	27.0	37.0
21	33.895105887840515	37.0	33.0	37.0	27.0	37.0
22	33.92349812661924	37.0	33.0	37.0	27.0	37.0
23	33.87775113188702	37.0	33.0	37.0	27.0	37.0
24	33.86326602006723	37.0	33.0	37.0	27.0	37.0
25	33.86233820226428	37.0	33.0	37.0	27.0	37.0
26	33.82579333492195	37.0	33.0	37.0	27.0	37.0
27	33.755715205564876	37.0	33.0	37.0	27.0	37.0
28	33.703559676936884	37.0	33.0	37.0	27.0	37.0
29	33.75445276494775	37.0	33.0	37.0	27.0	37.0
30	33.70150630966806	37.0	33.0	37.0	27.0	37.0
31	33.690686838676314	37.0	33.0	37.0	27.0	37.0
32	33.71268575368719	37.0	33.0	37.0	27.0	37.0
33	33.68823293803901	37.0	33.0	37.0	27.0	37.0
34	33.5722759928411	37.0	33.0	37.0	27.0	37.0
35	33.6164360642273	37.0	33.0	37.0	27.0	37.0
36	33.57325451107044	37.0	33.0	37.0	27.0	37.0
37	33.601763360829864	37.0	33.0	37.0	27.0	37.0
38	33.459731186339276	37.0	33.0	37.0	27.0	37.0
39	33.535629724645986	37.0	33.0	37.0	27.0	37.0
40	33.55074352175302	37.0	33.0	37.0	27.0	37.0
41	33.585554434512794	37.0	33.0	37.0	27.0	37.0
42	33.45876280819522	37.0	33.0	37.0	27.0	37.0
43	33.17283268352287	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	19.0
16	19.0
17	20.0
18	17.0
19	30.0
20	95.0
21	222.0
22	535.0
23	1028.0
24	1807.0
25	2856.0
26	4000.0
27	5441.0
28	6933.0
29	8712.0
30	10259.0
31	12658.0
32	15107.0
33	18778.0
34	24676.0
35	37799.0
36	46226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.742862647474865	22.055192484168792	13.11924233282802	24.082702535528323
2	16.24897965391889	25.340073110014856	36.14839000795997	22.26255722810629
3	18.580692263621938	28.07282609246744	30.959201366883494	22.38728027702713
4	14.254424879713238	20.04948361615721	37.39511349290447	28.300978011225077
5	14.886152192539939	37.634419505468045	34.623321182131136	12.856107119860877
6	28.548396092011135	40.67593808463929	17.660986528896707	13.114679294452866
7	25.05158768385242	33.54441610853947	23.849987578395535	17.55400862921257
8	23.587866373956203	37.02145135040586	21.018875768745215	18.371806506892725
9	25.562647981869528	16.158732894943647	20.32073089734685	37.95788822583998
10	14.460268610859018	29.371264012330343	34.22582983922895	21.942637537581692
11	32.803682878973014	24.419353366761815	24.665250434756157	18.11171331950902
12	22.087640757058765	26.698337533018652	30.558667998397866	20.65535371152471
13	27.27530838534352	22.811135841652426	27.31586872645599	22.597687046548064
14	21.483798678746886	22.001957036458677	28.216308299152796	28.297935985641637
15	23.521448815384538	29.51373221048789	24.846250956970547	22.118568017157024
16	22.268134275009253	29.486353980236974	25.497751436089576	22.747760308664198
17	22.182450554409165	28.577802339317675	26.53406815151315	22.705678954760007
18	21.892444115455014	28.263459695696042	28.147862723525506	21.696233465323445
19	24.075604475833643	27.23373403570324	28.33951033528192	20.3511511531812
20	23.813990275658217	26.84891779939869	28.29945699843336	21.03763492650973
21	22.287400437037675	28.33951033528192	27.334120879956604	22.038968347723802
22	21.755045959936524	28.038856806785745	27.696628928649293	22.50946830462844
23	21.946693571692936	27.95114506913003	27.86546134852994	22.23670001064709
24	23.07579206741129	27.988156380395157	27.15058533642268	21.785466215770874
25	22.907466651794543	27.41574856644544	27.930864898573798	21.745919883186218
26	22.574364850408394	27.615001242160446	27.609931199521387	22.200702707909773
27	22.571829829088863	27.436535741265583	27.463406967252595	22.52822746239296
28	21.67189726065596	27.840618139598554	28.255854631737453	22.231629968008033
29	22.776659551706828	27.96432717999158	27.3777232466525	21.881290021649082
30	22.116540000101402	28.19400011154094	27.696628928649293	21.99283095970837
31	22.632670340757567	27.541485623894097	28.04646187074433	21.779382164604005
32	21.716006631615773	27.959764141616432	27.711332052302563	22.61289717446524
33	22.115018987309686	27.582045965006564	28.17524095377642	22.12769409390733
34	22.04201037330724	27.814760922139353	27.560244781658618	22.58298392289479
35	22.18042253735354	28.179803992151577	27.808169866708575	21.831603603786306
36	22.63114932796585	27.919710804767867	27.352880037721118	22.09625982954517
37	22.94650598011529	27.17137251124282	27.341218939651284	22.540902568990607
38	22.88566546844659	27.864954344266035	27.44008477111293	21.80929541617445
39	22.41516551154195	27.44160578390464	27.821858981834037	22.321369722719368
40	22.287400437037675	27.795494760110934	27.814760922139353	22.102343880712034
41	21.843264701856143	27.574440901047975	28.349650420560035	22.232643976535844
42	22.445585767376304	27.32803682878973	27.710825048038657	22.51555235579531
43	22.117047004365308	27.35490805477674	27.77267956823517	22.755365372622784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	5.5
2	10.0
3	26.0
4	42.0
5	42.0
6	73.5
7	105.0
8	103.5
9	102.0
10	150.0
11	198.0
12	198.0
13	333.5
14	469.0
15	861.5
16	1254.0
17	1363.0
18	1472.0
19	1472.0
20	1495.5
21	1519.0
22	1805.5
23	2092.0
24	2642.5
25	3193.0
26	3193.0
27	3977.0
28	4761.0
29	6154.0
30	7547.0
31	8399.5
32	9252.0
33	9252.0
34	10113.5
35	10975.0
36	11781.5
37	12588.0
38	13708.0
39	14828.0
40	14828.0
41	15620.5
42	16413.0
43	15489.0
44	14565.0
45	14743.0
46	14921.0
47	14921.0
48	14626.0
49	14331.0
50	14718.0
51	15105.0
52	14917.0
53	14729.0
54	14729.0
55	12658.5
56	10588.0
57	9288.0
58	7988.0
59	6956.0
60	5924.0
61	5924.0
62	5107.5
63	4291.0
64	3513.5
65	2736.0
66	2341.0
67	1946.0
68	1946.0
69	1588.0
70	1230.0
71	1047.5
72	865.0
73	718.5
74	572.0
75	572.0
76	424.0
77	276.0
78	222.0
79	168.0
80	119.0
81	70.0
82	70.0
83	56.0
84	42.0
85	38.5
86	35.0
87	29.0
88	23.0
89	23.0
90	17.0
91	11.0
92	5.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	197237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.449162277793256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33398864166637	53.39715614044837
2	5.851507506056168	7.074374536066292
3	1.712579314429177	3.1057195467456373
4	0.8301972028284194	2.0073890174538067
5	0.5243653874301901	1.5848724198812743
6	0.3954235925827216	1.4341814949900562
7	0.3027249575854668	1.2809629060635372
8	0.2310135144169049	1.1171658737080652
9	0.16728312924806915	0.9100912523628237
>10	1.4677028627814714	17.752104879037223
>50	0.14338478424131704	5.937832621211357
>100	0.03783765139701421	3.63854011102105
>500	0.001991455336684959	0.7596092010105152
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	707	0.35845201458144266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.281387366467752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	340	0.17238144972799221	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	278	0.14094718536582893	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	251	0.12725807024037072	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	248	0.12573705744865316	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	220	0.11154093805928907	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	215	0.10900591673975978	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	213	0.10799190821194807	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	199	0.10089384851726603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.070042639058594E-4	0.0	0.0	0.0	0.0
3	5.070042639058594E-4	0.0	0.0	0.0	0.0
4	5.070042639058594E-4	0.0	5.070042639058594E-4	0.0	0.0
5	5.070042639058594E-4	0.0	5.070042639058594E-4	0.0	0.0
6	5.070042639058594E-4	0.0	0.0025350213195292973	0.0	0.0
7	5.070042639058594E-4	0.0	0.003042025583435157	0.0	0.0
8	5.070042639058594E-4	0.0	0.0035490298473410163	0.0	0.0
9	5.070042639058594E-4	0.0	0.0040560341112468755	5.070042639058594E-4	0.0
10	0.0010140085278117189	0.0	0.004563038375152735	5.070042639058594E-4	0.0
11	0.0010140085278117189	0.0	0.008112068222493751	5.070042639058594E-4	0.0
12	0.0010140085278117189	0.0	0.00912607675030547	5.070042639058594E-4	0.0
13	0.0010140085278117189	0.0	0.010647089542023048	5.070042639058594E-4	0.0
14	0.0010140085278117189	0.0	0.011661098069834768	5.070042639058594E-4	0.0
15	0.0010140085278117189	0.0	0.012675106597646486	5.070042639058594E-4	0.0
16	0.0010140085278117189	0.0	0.014196119389364065	5.070042639058594E-4	0.0
17	0.0010140085278117189	0.0	0.014703123653269925	5.070042639058594E-4	0.0
18	0.0010140085278117189	0.0	0.015717132181081644	5.070042639058594E-4	0.0
19	0.0010140085278117189	0.0	0.016224136444987502	5.070042639058594E-4	0.0
20	0.0010140085278117189	0.0	0.016224136444987502	5.070042639058594E-4	0.0
21	0.0010140085278117189	0.0	0.01774514923670508	5.070042639058594E-4	0.0
22	0.0010140085278117189	0.0	0.01825215350061094	5.070042639058594E-4	0.0
23	0.0010140085278117189	0.0	0.0187591577645168	0.0015210127917175784	0.0
24	0.0010140085278117189	0.0	0.020280170556234378	0.0015210127917175784	0.0
25	0.0010140085278117189	0.0	0.020280170556234378	0.0015210127917175784	0.0
26	0.0010140085278117189	0.0	0.020280170556234378	0.0015210127917175784	0.0
27	0.0010140085278117189	0.0	0.020280170556234378	0.0025350213195292973	0.0
28	0.0010140085278117189	0.0	0.020280170556234378	0.00963308101421133	0.0
29	0.0010140085278117189	0.0	0.020280170556234378	0.01825215350061094	0.0
30	0.0010140085278117189	0.0	0.020280170556234378	0.030927260098257428	0.0
31	0.0010140085278117189	0.0	0.020280170556234378	0.08821874191961955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCG	35	8.843255E-4	26.428572	24
ATACAAT	35	8.843255E-4	26.428572	6
TAATACA	50	9.041325E-6	25.899998	4
GGTATCA	160	0.0	24.28125	1
CTTCGCC	40	0.00192555	23.125	23
TTAGGCT	40	0.00192555	23.125	4
TTTAAGC	40	0.00192555	23.125	22
CCGGCAG	40	0.00192555	23.125	16
AGCTTCG	40	0.00192555	23.125	21
CGCTCTC	40	0.00192555	23.125	29
GCTTCGC	40	0.00192555	23.125	22
TAGGCTG	40	0.00192555	23.125	5
GGACTAC	50	2.691119E-4	22.199999	1
GCCGCTC	45	0.0038147455	20.555557	27
ACATAAT	45	0.0038147455	20.555557	1
GTATCAA	365	0.0	20.273972	1
CGGCAGC	55	5.122358E-4	20.181818	17
TGGCGGC	60	9.199878E-4	18.5	15
CTAATAC	80	1.6056325E-5	18.5	3
TCACGGT	50	0.00701474	18.499998	1
>>END_MODULE