FastQCFastQC Report
Fri 10 Feb 2017
ERR1633028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439314
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13050.29705404334940383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.20759638891544546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5310.12087026591458502No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4490.10220480112174891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3450.029.4927541
GCCGTCC451.321395E-424.66666627
TTCGCCG551.8991916E-523.54545424
TGCGGTA400.001929298423.12500236
GTCAACC802.716115E-823.12500215
CGCCGTC502.6984207E-422.226
GTGTTAC603.7195445E-521.5833341
GCCGGCA603.7195445E-521.58333415
TCGCTCG450.003822102720.55555537
GTATCAA8850.020.2768361
TCGCCGT555.1361445E-420.18181825
CGAGTCG555.1361445E-420.18181821
GTTAGGT1101.7462298E-920.1818181
GCCGCTC555.1361445E-420.18181827
TCCGCCG656.889732E-519.92307731
AGCTTCG656.889732E-519.92307721
CGCCGCT656.889732E-519.92307726
TAGGTAC1153.0358933E-919.3043463
GGTAACG500.007028132718.525
GCGGGTA500.007028132718.522
Produced by FastQC (version 0.11.5)