##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439314
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.732560309937767	33.0	33.0	33.0	27.0	33.0
2	31.239657739111433	33.0	33.0	33.0	27.0	33.0
3	31.662410030183423	33.0	33.0	33.0	27.0	33.0
4	31.800857700869994	33.0	33.0	33.0	27.0	33.0
5	31.918322657597983	33.0	33.0	33.0	33.0	33.0
6	34.81447438506399	37.0	37.0	37.0	27.0	37.0
7	34.987999471903926	37.0	37.0	37.0	33.0	37.0
8	35.16485475081605	37.0	37.0	37.0	33.0	37.0
9	35.26474230277205	37.0	37.0	37.0	33.0	37.0
10	35.27279804422349	37.0	37.0	37.0	33.0	37.0
11	35.30556048748731	37.0	37.0	37.0	33.0	37.0
12	35.260979618222954	37.0	37.0	37.0	33.0	37.0
13	35.282947959773644	37.0	37.0	37.0	33.0	37.0
14	35.27064468694373	37.0	37.0	37.0	33.0	37.0
15	35.287962596229576	37.0	37.0	37.0	33.0	37.0
16	35.27466686697897	37.0	37.0	37.0	33.0	37.0
17	35.24029737272202	37.0	37.0	37.0	33.0	37.0
18	35.25644527604401	37.0	37.0	37.0	33.0	37.0
19	35.26207450707239	37.0	37.0	37.0	33.0	37.0
20	35.26648820661304	37.0	37.0	37.0	33.0	37.0
21	35.258678302990575	37.0	37.0	37.0	33.0	37.0
22	35.265566314754366	37.0	37.0	37.0	33.0	37.0
23	35.2530080989907	37.0	37.0	37.0	33.0	37.0
24	35.25361586473456	37.0	37.0	37.0	33.0	37.0
25	35.24047719854136	37.0	37.0	37.0	33.0	37.0
26	35.20728909162922	37.0	37.0	37.0	33.0	37.0
27	35.14640553226166	37.0	37.0	37.0	33.0	37.0
28	35.10902907715211	37.0	37.0	37.0	33.0	37.0
29	35.158360534833854	37.0	37.0	37.0	33.0	37.0
30	35.12369512467165	37.0	37.0	37.0	33.0	37.0
31	35.11009665068721	37.0	37.0	37.0	33.0	37.0
32	35.136669898978866	37.0	37.0	37.0	33.0	37.0
33	35.112254560519354	37.0	37.0	37.0	33.0	37.0
34	35.061015583386826	37.0	37.0	37.0	33.0	37.0
35	35.05794716307698	37.0	37.0	37.0	27.0	37.0
36	35.050701775950685	37.0	37.0	37.0	27.0	37.0
37	35.07061691637416	37.0	37.0	37.0	27.0	37.0
38	34.92798317376637	37.0	37.0	37.0	27.0	37.0
39	34.99420687708564	37.0	37.0	37.0	27.0	37.0
40	35.025521608689914	37.0	37.0	37.0	27.0	37.0
41	35.042969265718824	37.0	37.0	37.0	27.0	37.0
42	34.96754030147002	37.0	37.0	37.0	27.0	37.0
43	34.76101376236587	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	26.0
17	13.0
18	15.0
19	20.0
20	34.0
21	79.0
22	219.0
23	565.0
24	1200.0
25	2026.0
26	3474.0
27	5258.0
28	7616.0
29	10272.0
30	13429.0
31	17329.0
32	22034.0
33	28598.0
34	40840.0
35	74036.0
36	212219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.973426751708345	20.179643717250077	13.457572488015407	25.389357043026173
2	16.09236218285782	22.77254993011832	37.818507946480196	23.316579940543665
3	17.658667832120077	26.7637726091133	30.184332846210225	25.393226712556395
4	12.83432806603022	17.824380739061354	39.0497457399491	30.291545454959323
5	14.011162858456593	36.862244317276485	35.389265991978405	13.737326832288524
6	29.760034963602344	39.7565294982632	17.26487205051512	13.218563487619333
7	26.720523361422583	31.925001251951908	23.701498244991054	17.65297714163446
8	24.538940256855003	35.952871977674285	20.653792048512	18.854395716958713
9	26.101148608967616	15.348019867338625	20.06947194944846	38.4813595742453
10	15.059615673527363	28.131131718998258	34.039206581169736	22.770046026304648
11	34.258411978675845	23.459302457923034	24.01607961503617	18.26620594836495
12	23.06505142107923	25.487236919378848	30.226899211042674	21.220812448499252
13	27.94561520916702	21.943757767792512	26.210182238672115	23.900444784368354
14	22.101276080434495	20.761232284880517	27.531788197052677	29.605703437632307
15	23.91159853772017	28.59617494548318	24.231642970631484	23.260583546165154
16	23.14335532216137	28.1049545427644	25.156949243593417	23.594740891480807
17	22.576790177412967	27.590060867625432	26.23704229776424	23.596106657197357
18	23.092594363029633	27.206053073655745	27.436184596894247	22.265167966420375
19	24.872869974551232	26.159193651920948	26.915372603650233	22.05256376987758
20	24.217530058227148	26.426883732364548	27.088141966793682	22.267444242614623
21	23.388055013043065	26.83593056447097	27.038291518139644	22.73772290434632
22	23.214375139421918	26.68410294231461	26.860514347368852	23.241007570894624
23	23.04911748771949	26.67613597563474	27.38428549966539	22.890461036980383
24	23.262632194739982	27.052404430543987	26.230896352039775	23.454067022676263
25	23.404216574022225	26.6941185575693	27.265463882325626	22.636200986082848
26	23.48115470938782	26.84526329686739	26.992310739015828	22.681271254728962
27	23.277883245241444	26.87917981216169	26.507691537260364	23.3352454053365
28	22.908898874153792	26.62469213364473	27.528373782761307	22.938035209440173
29	24.005836372162054	26.46603568290562	26.839117351142917	22.68901059378941
30	23.571522874299475	26.4318915399919	27.133667490678647	22.86291809502998
31	23.386461619707088	26.689566005180804	27.047396622916636	22.876575752195468
32	22.6018292155497	26.439403251432914	27.184883705049234	23.773883827968152
33	22.745006988167916	26.544111956368337	27.56342843615273	23.147452619311018
34	23.400574532111428	26.188557614826752	27.261594212795405	23.149273640266415
35	23.231902466117628	26.85527891212208	26.98320563423884	22.929612987521452
36	23.83329463663803	26.549347391615107	26.584857300245385	23.032500671501477
37	23.3040604214753	26.349945596998957	26.863018251182524	23.482975730343217
38	23.354138497748764	26.579849492618035	26.841621254956593	23.22439075467661
39	22.749104285317564	26.617180422203706	27.24907469372704	23.38464059875169
40	22.92096313798331	26.793591827257952	27.52245546465626	22.76298957010248
41	22.937579954201322	26.023299963124323	27.458264475978456	23.580855606695895
42	22.60911329937129	26.993221249493526	27.125472896379353	23.272192554755826
43	23.446555311235244	25.867147416198893	27.239741961330623	23.446555311235244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	15.0
2	23.0
3	78.0
4	133.0
5	133.0
6	169.0
7	205.0
8	204.5
9	204.0
10	265.0
11	326.0
12	326.0
13	533.5
14	741.0
15	1232.5
16	1724.0
17	2024.0
18	2324.0
19	2324.0
20	2300.5
21	2277.0
22	2753.0
23	3229.0
24	4247.0
25	5265.0
26	5265.0
27	6815.5
28	8366.0
29	10541.0
30	12716.0
31	15089.0
32	17462.0
33	17462.0
34	20115.5
35	22769.0
36	25013.0
37	27257.0
38	29422.0
39	31587.0
40	31587.0
41	33078.5
42	34570.0
43	35080.5
44	35591.0
45	34811.5
46	34032.0
47	34032.0
48	33117.0
49	32202.0
50	32058.5
51	31915.0
52	30860.0
53	29805.0
54	29805.0
55	28521.5
56	27238.0
57	23786.5
58	20335.0
59	18653.0
60	16971.0
61	16971.0
62	15304.0
63	13637.0
64	11093.5
65	8550.0
66	7277.5
67	6005.0
68	6005.0
69	5027.0
70	4049.0
71	3421.5
72	2794.0
73	2480.0
74	2166.0
75	2166.0
76	1799.5
77	1433.0
78	1058.5
79	684.0
80	493.5
81	303.0
82	303.0
83	234.5
84	166.0
85	145.5
86	125.0
87	104.0
88	83.0
89	83.0
90	60.0
91	37.0
92	21.0
93	5.0
94	4.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	439314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.4680757512083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24170060108008	59.31724115719241
2	5.961606540602767	7.92513030279367
3	1.662944606113902	3.315981841477263
4	0.740772096232737	1.969507832271157
5	0.4471573476385081	1.486084422777287
6	0.3047221969951936	1.2152578843770685
7	0.2330497001075826	1.0843255584383023
8	0.1686410397588512	0.896739632436308
9	0.13428811991709816	0.8033285634423323
>10	0.9646891180465944	12.370297093217875
>50	0.10351004929651214	4.648204880637427
>100	0.035883483718427825	4.3376824077360885
>500	6.900669945851505E-4	0.3309334733194023
>1k	3.4503349729257524E-4	0.29928494988345117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.29705404334940383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.20759638891544546	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.12087026591458502	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	449	0.10220480112174891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.552552388496611E-4	0.0	0.0
2	0.0	0.0	4.552552388496611E-4	0.0	0.0
3	0.0	0.0	6.828828582744916E-4	0.0	0.0
4	0.0	0.0	6.828828582744916E-4	0.0	0.0
5	2.2762761942483054E-4	0.0	9.105104776993222E-4	2.2762761942483054E-4	0.0
6	2.2762761942483054E-4	0.0	0.0015933933359738137	2.2762761942483054E-4	0.0
7	2.2762761942483054E-4	0.0	0.002276276194248305	4.552552388496611E-4	0.0
8	2.2762761942483054E-4	0.0	0.002503903813673136	4.552552388496611E-4	0.0
9	2.2762761942483054E-4	0.0	0.0031867866719476274	9.105104776993222E-4	0.0
10	4.552552388496611E-4	0.0	0.003869669530222119	9.105104776993222E-4	0.0
11	4.552552388496611E-4	0.0	0.007284083821594577	0.0011381380971241526	0.0
12	4.552552388496611E-4	0.0	0.00842222191871873	0.0011381380971241526	0.0
13	4.552552388496611E-4	0.0	0.00910510477699322	0.0011381380971241526	0.0
14	4.552552388496611E-4	0.0	0.010470870493542204	0.0011381380971241526	0.0
15	6.828828582744916E-4	0.0	0.01274714668779051	0.0011381380971241526	0.0
16	6.828828582744916E-4	0.0	0.013430029546065002	0.0011381380971241526	0.0
17	6.828828582744916E-4	0.0	0.013657657165489832	0.0011381380971241526	0.0
18	6.828828582744916E-4	0.0	0.013657657165489832	0.0011381380971241526	0.0
19	6.828828582744916E-4	0.0	0.013885284784914662	0.0011381380971241526	0.0
20	6.828828582744916E-4	0.0	0.014112912404339492	0.0011381380971241526	0.0
21	6.828828582744916E-4	0.0	0.014568167643189155	0.0011381380971241526	2.2762761942483054E-4
22	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.0011381380971241526	2.2762761942483054E-4
23	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.0013657657165489832	2.2762761942483054E-4
24	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.0013657657165489832	2.2762761942483054E-4
25	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.0015933933359738137	2.2762761942483054E-4
26	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.0018210209553986443	2.2762761942483054E-4
27	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.002503903813673136	2.2762761942483054E-4
28	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.007284083821594577	2.2762761942483054E-4
29	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.018437837173411274	2.2762761942483054E-4
30	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.04142822673531916	2.2762761942483054E-4
31	6.828828582744916E-4	2.2762761942483054E-4	0.015023422882038815	0.10379819445772272	2.2762761942483054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	345	0.0	29.492754	1
GCCGTCC	45	1.321395E-4	24.666666	27
TTCGCCG	55	1.8991916E-5	23.545454	24
TGCGGTA	40	0.0019292984	23.125002	36
GTCAACC	80	2.716115E-8	23.125002	15
CGCCGTC	50	2.6984207E-4	22.2	26
GTGTTAC	60	3.7195445E-5	21.583334	1
GCCGGCA	60	3.7195445E-5	21.583334	15
TCGCTCG	45	0.0038221027	20.555555	37
GTATCAA	885	0.0	20.276836	1
TCGCCGT	55	5.1361445E-4	20.181818	25
CGAGTCG	55	5.1361445E-4	20.181818	21
GTTAGGT	110	1.7462298E-9	20.181818	1
GCCGCTC	55	5.1361445E-4	20.181818	27
TCCGCCG	65	6.889732E-5	19.923077	31
AGCTTCG	65	6.889732E-5	19.923077	21
CGCCGCT	65	6.889732E-5	19.923077	26
TAGGTAC	115	3.0358933E-9	19.304346	3
GGTAACG	50	0.0070281327	18.5	25
GCGGGTA	50	0.0070281327	18.5	22
>>END_MODULE