FastQCFastQC Report
Fri 10 Feb 2017
ERR1633027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253841
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5150.202882907016597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1536394829834424No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3090.1217297442099582No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2980.11739632289504061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCA358.850288E-426.428578
TATAAGA358.850288E-426.428572
TAACGGC751.3629688E-824.66666636
GGTATCA1200.024.6666661
TTAACGG751.3629688E-824.66666635
GTATCAA3450.024.1304361
TGCGAAT400.001927066223.12513
AGGGGTT802.7046553E-823.1255
GGCTTAT502.6940714E-422.21
CTTAGGT502.6940714E-422.24
GTCGCCC957.0795068E-921.42105337
AACGGCC957.0795068E-921.42105337
GCTCGGA957.0795068E-921.42105311
GTGTTTA450.003817722220.5555551
TTTAACG909.4054485E-820.55555534
GCTCGTC450.003817722220.55555512
CTTACAC450.003817722220.5555553
TATGGGA555.127933E-420.1818182
CTTGCTA555.127933E-420.18181816
TAGGGGT555.127933E-420.1818184
Produced by FastQC (version 0.11.5)