FastQCFastQC Report
Fri 10 Feb 2017
ERR1633026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555806
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40710.7324498116249195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.56494532264855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16450.29596657826651745No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13770.24774831505957115No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13680.24612904502650207No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13270.2387523704314095No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12810.2304761013735008No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12760.22957650691068465No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12750.2293965880181214No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12510.22507853459660382No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11930.21464323882793637No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11880.21374364436512022No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11810.21248421211717758No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11480.2065468886625909No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11390.2049276186295218No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11160.20078948410056746No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10970.19737102514186608No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10970.19737102514186608No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10550.1898144316542103No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10340.1860361349103824No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10120.18207791927399128No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9500.17092294793507087No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9360.16840408343918561No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC9180.16516554337304742No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9100.1637261922325416No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG9060.16300651666228866No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9030.16246675998459895No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8990.16174708441434602No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8940.16084748995152987No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8700.15652943653001228No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8500.15293105867874762No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG8500.15293105867874762No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8070.14519454629852863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.14375519515802276No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7960.14321543848033305No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7920.14249576291008012No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7690.1383576283811258No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7670.13799779059599934No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7620.13709819613318316No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7560.13601868277780377No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7460.13421949385217144No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT7290.1311608726785965No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7240.13026127821578032No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7160.12882192707527446No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7040.12666290036451566No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7040.12666290036451566No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6920.1245038736537569No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6520.11730711795122759No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6510.11712719905866435No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6480.11658744238097465No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6460.11622760459584819No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6440.11586776681072172No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6210.11172963228176738No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6180.1111898756040777No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6110.10993044335613505No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6020.10831117332306597No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5990.10777141664537626No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5880.10579230882718071No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5860.10543247104205425No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5840.10507263325692778No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5830.10489271436436454No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5800.10435295768667485No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5780.10399311990154839No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5770.10381320100898514No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5760.10363328211642192No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5720.10291360654616899No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG5690.10237384986847928No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5620.10111441762053666No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5590.10057466094284695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT200.001841118337.037
CCCACGA200.001841118337.019
GCTCTAG251.2328052E-437.01
CAGGATC200.001841118337.033
CGAGACA200.001841118337.023
GGTATCA8150.031.7791441
CCCAGGG250.00549393129.69
ATAGACC250.00549393129.63
TACCTTA250.00549393129.631
CGAGCCC250.00549393129.615
TATAGAC250.00549393129.62
GCTGTGG250.00549393129.68
CGTCCCA250.00549393129.631
AATCGTC250.00549393129.628
ATAGATC454.003363E-628.7777776
ACTAGTG454.003363E-628.7777778
CTAGTGC405.9346952E-527.759
TAGTGCA405.9346952E-527.7510
GTGACAT405.9346952E-527.7516
GGCAACT604.314461E-827.74999830
Produced by FastQC (version 0.11.5)