FastQCFastQC Report
Fri 10 Feb 2017
ERR1633025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417033
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31050.7445453956881111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.563744355962238No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12690.3042924660638367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12230.2932621639054943No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10870.26065083578517767No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10760.2580131548342697No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9880.23691170722700602No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9870.23667191804965074No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9710.23283529121196642No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9470.22708035095543996No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9410.22564161589130835No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9160.21964688645742664No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9130.2189275189253608No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9050.21700920550651864No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9000.21581025961974232No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8980.2153306812650318No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8500.20382080075197886No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7890.18919366093330744No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7850.18823450422388635No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7810.18727534751446528No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7740.18559682327297838No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7710.18487745574091258No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7350.1762450453561229No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7350.1762450453561229No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7130.17096968345430696No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7040.16881158085810954No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7030.16857179168075428No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6990.16761263497133322No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6980.16737284579397793No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6980.16737284579397793No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6810.16329642977893835No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6670.1599393812959646No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6620.15874043540918825No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6610.158500646231833No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5990.14363371723580626No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5940.14243477134902993No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5890.14123582546225358No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5860.14051645793018777No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5790.1388379336887009No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5600.13428193931895077No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5560.1333227826095297No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5540.13284320425481916No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5520.1323636259001086No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5340.12804742070771377No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5280.12660868564358216No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5100.12229248045118732No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5070.12157311291912151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.12157311291912151No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5040.1208537453870557No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5040.1208537453870557No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5020.12037416703234516No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4840.11605796183995032No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4770.11437943759846343No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4700.11270091335697655No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4690.11246112417962129No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT4670.11198154582491074No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT4630.11102238911548966No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG4580.10982344322871332No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT4510.10814491898722643No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG4510.10814491898722643No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4490.1076653406325159No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4450.10670618392309482No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4440.10646639474573955No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4420.10598681639102901No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4400.10550723803631848No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4370.10478787050425267No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT4340.10406850297218687No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4310.10334913544012105No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4300.10310934626276579No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4270.10238997873069998No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA4210.10095124366656837No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4190.10047166531185782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGA403.823334E-837.04
ATAGATC251.2322771E-436.9999966
TAGATCT251.2322771E-436.9999967
GACATCT251.2322771E-436.99999618
TATAGAT451.07716914E-732.888895
AGATCTT303.5955795E-430.8333348
GTGACAT303.5955795E-430.83333416
GAATCAA502.7137503E-729.5999981
TGAGCAC250.00549237829.59999812
AAGCTAG250.00549237829.5999985
TGCACCG405.9311948E-527.755
GGTATCA5300.027.575471
TCTAAGG358.859881E-426.4285722
AGCTGCG358.859881E-426.4285713
GATCTTG358.859881E-426.428579
GGACAGA358.859881E-426.428576
GACTCGA358.859881E-426.4285711
GCCGCTC1150.025.7391327
TGACATC451.3212377E-424.66666617
GCCGGCA1150.024.13043615
Produced by FastQC (version 0.11.5)