FastQCFastQC Report
Fri 10 Feb 2017
ERR1633024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536554
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26240.4890467688247595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.351688739623598No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC18130.33789702434424124No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17750.3308147921737607No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG16770.31255008815515306No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15430.28757590102766917No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG15320.28552578118884586No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG15310.28533940665804375No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14140.26353358655419584No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC14090.2626017139001853No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13970.2603652195305598No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12950.24135501738874374No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12920.24079589379633737No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT12900.2404231447347331No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT12880.24005039567312889No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12270.22868154929419965No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11560.21544895760724922No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11430.2130260887068217No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11150.20780760184436237No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT11050.20594385653634117No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC10530.196252380934631No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG10250.1910338940721717No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG10200.19010202141816107No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG10190.18991564688735896No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10060.18749277798693142No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10030.18693365439452506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9890.18432441096329538No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9720.18115604393965937No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC9650.17985142222404454No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9190.1712781938071471No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA8910.16605970694468777No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8700.16214584179784328No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG8700.16214584179784328No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT8180.1524543661961331No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT8080.15059062088811193No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8060.15021787182650767No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7800.14537213402565258No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7750.14444026137164198No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7570.14108551981720385No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC7480.13940814903998477No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7290.13586703295474453No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG7120.13269866593110852No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC7090.13213954233870215No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC7040.13120766968469155No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7010.1306485460922852No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG6860.12785292813025342No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT6840.1274801790686492No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT6800.12673468094544074No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA6770.12617555735303437No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6770.12617555735303437No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6750.12580280829143012No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6620.12337993939100257No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA6590.12282081579859623No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6470.1205843214289708No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6340.11816145252854326No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA6320.11778870346693902No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG6300.11741595440533478No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT6270.11685683081292843No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT6260.11667045628212631No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6250.11648408175132419No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC6190.11536583456651148No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT6160.11480671097410514No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6100.11368846378929241No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG6090.1135020892584903No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6050.11275659113528182No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6030.11238384207367758No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5890.10977459864244793No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT5500.1025059919411653No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC5470.10194686834875893No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5380.10026949757153986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAAC303.596943E-430.83333433
GCTGCCG250.00549376429.619
TGTACTT250.00549376429.65
GTTGCGG250.00549376429.69
GGTATCA4750.028.0421051
TGTACTA556.2513027E-726.909095
TTAGCTT856.91216E-1126.11764733
ATTTTCC509.079295E-625.90000210
CATTTTC509.079295E-625.9000029
TTGTACT659.372343E-825.6153854
CGTAGTT601.3351873E-624.66666828
TCTATAA451.321928E-424.66666624
GGTCTTA1002.0008883E-1124.05000129
GCCGTCC851.9699655E-923.94117727
GTGTTTC551.9002087E-523.54545437
CGAGTCG1103.6379788E-1223.54545421
ATCTATA400.001929852223.12500223
TTTGATA400.001929852223.12500237
CAAGCAT1850.023.037
CCAAGCA1850.023.036
Produced by FastQC (version 0.11.5)