FastQCFastQC Report
Fri 10 Feb 2017
ERR1633023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465226
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11120.23902361432937969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.17797801498626473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG250.00549302329.59999821
CGTCCGC250.00549302329.59999829
GGTATCA2250.027.1333331
GATAGCG358.861426E-426.42857225
GTATCAA6350.023.3070871
ATAGCGA400.001929468423.12500226
TTGACGC400.001929468423.12500211
GGCCGTT603.7201506E-521.58333414
ATCCGTA450.003822437320.55555512
TACACCC555.1367696E-420.1818185
GCCGTTA656.8908455E-519.92307715
CTAATAG759.246338E-619.7333343
ACGCCGG500.00702874318.49999814
GGGCTAC500.00702874318.49999820
GTATAAT1351.1459633E-917.8148161
TTTACAC1156.380651E-817.6956523
GGTACCG752.0639347E-417.2666666
TTAGCCG650.00157802317.07692329
GTATAGA650.00157802317.0769231
CGTTAAA803.3771532E-416.187517
Produced by FastQC (version 0.11.5)