FastQCFastQC Report
Fri 10 Feb 2017
ERR1633022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457203
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33970.7429959995888041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.5063396346918109No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14860.3250197395905101No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13890.3038037808150865No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13050.28543119795801863No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13010.284556313060063No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12750.27886956122335155No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12550.2744951367335735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12300.26902710612135095No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT12120.26509012408055066No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12120.26509012408055066No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11920.26071569959077256No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11530.25218557183570534No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11090.24256183795819364No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10820.23665636489699324No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10700.23403171020312638No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10320.22572030367254808No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10270.22462669755010356No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9930.21719017591748085No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9700.21215958775423607No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC9550.20887876938690256No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8790.1922559563257459No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8460.18503815591761208No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8300.18153861632578963No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8270.18088245265232292No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8160.178476519182945No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8130.17782035550947828No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7990.17475825836663364No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT7930.1734459310197002No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7890.1725710461217446No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7830.1712587187748112No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7780.17016511265236667No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7770.16994639142787776No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7700.16841534285645543No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC7520.1644783608156552No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7420.16229114857076618No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7340.16054137877485494No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7330.16032265755036604No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7220.15791672408098809No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7140.15616695428507688No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7080.15485462693814345No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6960.15222997224427662No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6770.14807426897898746No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6600.14435600816267607No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6440.14085646857085365No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA6220.13604460163209778No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG6090.13320122571374204No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6040.13210761959129752No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT6030.13188889836680862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.1312327346933419No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT5970.1305765710198752No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5950.13013912857089738No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5920.12948296489743066No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5740.12554598285663043No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5730.12532726163214153No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG5710.12488981918316372No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC5700.12467109795867481No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5660.1237962130607192No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5620.12292132816276358No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT5580.12204644326480799No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5530.12095283714236346No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT5510.12051539469338565No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5490.12007795224440784No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5480.11985923101991894No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5480.11985923101991894No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC5470.11964050979543005No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA5450.11920306734645224No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5420.11854690367298552No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5370.117453297550541No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA5320.1163596914280965No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5310.1161409702036076No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA5160.11286015183627404No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG5150.11264143061178514No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT5130.11220398816280733No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT5090.11132910326485171No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5020.1097980546934294No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT5010.1095793334689405No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC4980.10892316979547378No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC4950.10826700612200707No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG4860.10629851510160694No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG4840.10586107265262913No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC4710.1030176967342734No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA4660.10192409061182887No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT4620.10104920571387327No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4620.10104920571387327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCA200.001840776937.09
CTCGCTA200.001840776937.013
ATAGATG250.00549292429.66
ATGCACT250.00549292429.611
CCGCATG250.00549292429.622
GCACTAC250.00549292429.613
TACATAC454.0013183E-628.7777773
GGTATCA6250.028.121
TGCGGGT754.6020432E-1027.13333321
CGTCCTA556.247901E-726.9090933
TCTGCGG751.3691533E-824.66666619
ATACTTC601.3344652E-624.6666666
CTGCGGG751.3691533E-824.66666620
TTCTGCG751.3691533E-824.66666618
GTATCAA18350.023.6920971
CAGTATT1800.023.6388914
CATACTT551.8994113E-523.5454545
ATTAGAT400.001929418223.1250023
TCTTCTG802.7166607E-823.12500216
TTAGATA400.001929418223.1250024
Produced by FastQC (version 0.11.5)