FastQCFastQC Report
Fri 10 Feb 2017
ERR1633021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187733
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4170.2221239739417151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.17951026191452754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2100.11186099407136732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA250.005484780829.59999823
GGTATCA1350.027.4074081
CATGCTA400.001925205823.1254
GTATCAA3650.021.287671
TAATATA450.003814071620.5555554
TACCGCA609.1976265E-418.57
GTTTGGT500.007013510418.49999837
GACCAAC500.007013510418.49999829
TCAATAC500.007013510418.4999983
TCAGATC500.007013510418.49999820
AATAATA500.007013510418.4999982
CATATTC650.001573285317.0769233
TCTTGGA700.002581674415.8571422
GAACTCA700.002581674415.85714216
ATATTCA700.002581674415.8571424
CAACGCA5150.015.0873795
TCAACGC5150.015.0873794
AACGCAG5200.014.9423076
GTACATG750.00408741814.81
ACTACAC750.00408741814.83
Produced by FastQC (version 0.11.5)