##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187733
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.561670031374344	33.0	33.0	33.0	14.0	33.0
2	30.404792977260257	33.0	33.0	33.0	27.0	33.0
3	30.884287791704175	33.0	33.0	33.0	27.0	33.0
4	31.06772916855321	33.0	33.0	33.0	27.0	33.0
5	31.17716118103903	33.0	33.0	33.0	27.0	33.0
6	33.547889822247555	37.0	33.0	37.0	27.0	37.0
7	33.78426275614836	37.0	33.0	37.0	27.0	37.0
8	33.89678958947015	37.0	33.0	37.0	27.0	37.0
9	33.98272013977297	37.0	33.0	37.0	27.0	37.0
10	34.00756926059883	37.0	33.0	37.0	27.0	37.0
11	33.98553264476677	37.0	33.0	37.0	27.0	37.0
12	34.071676263629726	37.0	33.0	37.0	27.0	37.0
13	34.064762188853315	37.0	33.0	37.0	27.0	37.0
14	34.05079554473641	37.0	33.0	37.0	27.0	37.0
15	34.11612236527409	37.0	33.0	37.0	27.0	37.0
16	34.069220648474165	37.0	33.0	37.0	27.0	37.0
17	34.08560029403461	37.0	33.0	37.0	27.0	37.0
18	34.083943685979555	37.0	33.0	37.0	27.0	37.0
19	34.06709528958681	37.0	33.0	37.0	27.0	37.0
20	34.07520254829998	37.0	33.0	37.0	27.0	37.0
21	34.087922741340094	37.0	33.0	37.0	27.0	37.0
22	34.08187159423223	37.0	33.0	37.0	27.0	37.0
23	34.049208183963394	37.0	33.0	37.0	27.0	37.0
24	34.0446804770605	37.0	33.0	37.0	27.0	37.0
25	34.026606936446974	37.0	33.0	37.0	27.0	37.0
26	33.98471765752425	37.0	33.0	37.0	27.0	37.0
27	33.91286028561841	37.0	33.0	37.0	27.0	37.0
28	33.81814065720997	37.0	33.0	37.0	27.0	37.0
29	33.908279311575484	37.0	33.0	37.0	27.0	37.0
30	33.86120713992745	37.0	33.0	37.0	27.0	37.0
31	33.86039215268493	37.0	33.0	37.0	27.0	37.0
32	33.87467307292804	37.0	33.0	37.0	27.0	37.0
33	33.85263113038198	37.0	33.0	37.0	27.0	37.0
34	33.75948288260455	37.0	33.0	37.0	27.0	37.0
35	33.7567342981788	37.0	33.0	37.0	27.0	37.0
36	33.736008053991576	37.0	33.0	37.0	27.0	37.0
37	33.77733270122994	37.0	33.0	37.0	27.0	37.0
38	33.66845999371448	37.0	33.0	37.0	27.0	37.0
39	33.7109778248896	37.0	33.0	37.0	27.0	37.0
40	33.73116607096249	37.0	33.0	37.0	27.0	37.0
41	33.74987349054242	37.0	33.0	37.0	27.0	37.0
42	33.64159737499534	37.0	33.0	37.0	27.0	37.0
43	33.367532612806485	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	13.0
17	15.0
18	8.0
19	14.0
20	70.0
21	194.0
22	422.0
23	897.0
24	1596.0
25	2458.0
26	3605.0
27	4836.0
28	6111.0
29	7677.0
30	9491.0
31	11152.0
32	13679.0
33	16956.0
34	22912.0
35	35614.0
36	50010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41377914378399	21.441621877879754	12.944447699658557	25.200151278677694
2	16.66515743103237	23.988323842904553	35.93454533832624	23.41197338773684
3	18.913563411866853	26.92866997278049	29.656480213920833	24.50128640143182
4	13.886210735459404	19.274714621297267	37.69875301625181	29.14032162699153
5	15.43521916764767	36.721300996628194	34.59274608087017	13.250733754853968
6	30.224840598083446	39.62436012848034	17.060932281484874	13.089866991951334
7	26.773129923881257	32.25538397617893	23.16534652937949	17.806139570560315
8	24.75164195959155	36.12683971384892	20.384269148205163	18.737249178354364
9	25.856402443896386	15.459189380662963	20.211150943094715	38.47325723234594
10	14.733158261999755	28.50804067478813	34.02012432550484	22.738676737707276
11	34.41217047615496	23.379480432316107	23.948373487879064	18.259975603649863
12	22.885161372800734	25.525613504285342	30.409677574001375	21.17954754891255
13	27.984957359654405	22.351957301060548	26.020997906601394	23.64208743268365
14	22.00305753383795	20.837039838494032	27.28662515380887	29.87327747385915
15	23.623443933671755	29.04816947473273	23.85036195021653	23.47802464137898
16	23.29798170806411	28.39191830951404	24.775079501206502	23.535020481215344
17	22.454762881326136	28.154879536362813	26.23832783794005	23.152029744370996
18	23.186653385393086	27.444295888309462	27.359601135655424	22.00944959064203
19	24.512472500838957	26.705480656038095	26.888719617755	21.89332722536794
20	23.972876372294696	26.687369828426544	27.228031299771484	22.11172249950728
21	23.393862560125285	27.048521037856954	27.026148839042683	22.531467562975077
22	22.755722222518152	26.95903224259986	27.086873378681425	23.198372156200563
23	22.985303596064625	26.643158102198335	27.33722893684115	23.03430936489589
24	23.060410263512544	27.24774014158406	26.11155204465917	23.580297550244232
25	23.44180298615587	27.104451534892636	26.93506202958457	22.518683449366918
26	23.562186722632674	26.852497962531896	26.73531025445713	22.850005060378304
27	23.326213292282123	26.92014723037505	26.4902814103008	23.263358067042024
28	22.951212626442874	26.929202644180833	27.106582220494	23.013002508882295
29	23.60906180586258	26.638364059595276	26.743832996862565	23.00874113767958
30	23.299579722265133	26.777391295083973	27.168372102933418	22.754656879717473
31	23.465240527770824	26.570182120351777	27.08207933607837	22.882498015799033
32	22.769571678926987	26.72359148364965	26.870076118743107	23.636760718680254
33	22.854799102981364	26.79869815109757	27.460808701719998	22.88569404420107
34	23.286262937256634	26.37469171642705	27.037867609850156	23.30117773646615
35	23.020459908487055	27.15239196092323	26.870076118743107	22.957072011846613
36	23.806682895388665	26.72146079804829	26.680977771622466	22.790878534940582
37	23.213819626810416	26.393335215438945	26.982469784214814	23.410375373535818
38	23.459913813767425	26.911624487969615	26.778456637884656	22.850005060378304
39	22.814848747955875	26.907895788167234	27.127356405107257	23.149899058769634
40	23.253237310435566	27.193407658749397	27.114572291499094	22.438782739315943
41	22.918719671022142	26.50679422371133	27.386767377072758	23.187718728193765
42	22.887824729802432	26.85409597673291	26.943052100589664	23.315027192874986
43	23.218613669413475	26.330479990198846	27.23602137077658	23.214884969611095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	20.0
4	35.0
5	35.0
6	46.5
7	58.0
8	67.5
9	77.0
10	106.0
11	135.0
12	135.0
13	220.0
14	305.0
15	511.0
16	717.0
17	793.5
18	870.0
19	870.0
20	914.5
21	959.0
22	1144.5
23	1330.0
24	1805.0
25	2280.0
26	2280.0
27	3130.5
28	3981.0
29	4775.5
30	5570.0
31	6526.0
32	7482.0
33	7482.0
34	8701.5
35	9921.0
36	10709.5
37	11498.0
38	12424.0
39	13350.0
40	13350.0
41	13978.5
42	14607.0
43	14915.0
44	15223.0
45	14950.5
46	14678.0
47	14678.0
48	14395.5
49	14113.0
50	13888.0
51	13663.0
52	13067.0
53	12471.0
54	12471.0
55	11981.0
56	11491.0
57	10177.5
58	8864.0
59	8116.0
60	7368.0
61	7368.0
62	6535.5
63	5703.0
64	4810.0
65	3917.0
66	3230.5
67	2544.0
68	2544.0
69	2153.0
70	1762.0
71	1474.5
72	1187.0
73	945.0
74	703.0
75	703.0
76	526.0
77	349.0
78	282.0
79	215.0
80	160.5
81	106.0
82	106.0
83	92.5
84	79.0
85	72.0
86	65.0
87	46.0
88	27.0
89	27.0
90	22.5
91	18.0
92	12.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	187733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.16024875870173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.27039447816367	58.80211548142963
2	7.668164196517359	10.453279583143866
3	2.1887469911940074	4.475566181689305
4	1.1306684218952843	3.0826656359996516
5	0.6017640961894801	2.0508195245165135
6	0.4089165390821774	1.6723111815233151
7	0.31243076052205976	1.4906750849937769
8	0.22906692183166494	1.2490606699549092
9	0.16459975704976387	1.0097244347520395
>10	0.960798130736896	11.773681617945227
>50	0.049576697552015166	2.3160762898945984
>100	0.014873009265604548	1.6240243141571988
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.2221239739417151	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	337	0.17951026191452754	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.11186099407136732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	5.326714003398444E-4	0.0	0.0
7	0.0	0.0	0.0010653428006796887	0.0	0.0
8	0.0	0.0	0.0010653428006796887	0.0	0.0
9	0.0	0.0	0.0010653428006796887	0.0	0.0
10	0.0	0.0	0.001598014201019533	0.0	0.0
11	0.0	0.0	0.002663357001699222	0.0	0.0
12	0.0	0.0	0.002663357001699222	0.0	0.0
13	0.0	0.0	0.002663357001699222	5.326714003398444E-4	0.0
14	0.0	0.0	0.002663357001699222	5.326714003398444E-4	0.0
15	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
16	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
17	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
18	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
19	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
20	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
21	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
22	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
23	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
24	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
25	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
26	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
27	0.0	0.0	0.003196028402039066	5.326714003398444E-4	0.0
28	0.0	0.0	0.003196028402039066	0.006392056804078132	0.0
29	0.0	0.0	0.003196028402039066	0.016512813410535176	0.0
30	0.0	0.0	0.003196028402039066	0.023970213015292997	0.0
31	0.0	0.0	0.003196028402039066	0.06392056804078132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGAA	25	0.0054847808	29.599998	23
GGTATCA	135	0.0	27.407408	1
CATGCTA	40	0.0019252058	23.125	4
GTATCAA	365	0.0	21.28767	1
TAATATA	45	0.0038140716	20.555555	4
TACCGCA	60	9.1976265E-4	18.5	7
GTTTGGT	50	0.0070135104	18.499998	37
GACCAAC	50	0.0070135104	18.499998	29
TCAATAC	50	0.0070135104	18.499998	3
TCAGATC	50	0.0070135104	18.499998	20
AATAATA	50	0.0070135104	18.499998	2
CATATTC	65	0.0015732853	17.076923	3
TCTTGGA	70	0.0025816744	15.857142	2
GAACTCA	70	0.0025816744	15.857142	16
ATATTCA	70	0.0025816744	15.857142	4
CAACGCA	515	0.0	15.087379	5
TCAACGC	515	0.0	15.087379	4
AACGCAG	520	0.0	14.942307	6
GTACATG	75	0.004087418	14.8	1
ACTACAC	75	0.004087418	14.8	3
>>END_MODULE