FastQCFastQC Report
Fri 10 Feb 2017
ERR1633020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426043
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11150.2617106723969177No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA10680.250678922080635No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCA8600.20185755897878851No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCA7600.17838574979520846No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCA7350.17251779749931345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.17204836131564186No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA6780.1591388662646728No Hit
GGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCTATTGGCA5950.13965726464230135No Hit
CTTTATTGGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCT4740.11125637553016948No Hit
CTTTATTGGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCT4410.10351067849958806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTT454.000476E-628.77777730
GTACATG706.5665517E-926.428571
CCTACGC358.8601967E-426.428573
TGACGGT509.073559E-625.90000228
GTATCAA7150.025.0979021
CGGTTTC601.3341069E-624.66666831
TGCGGGC451.3213033E-424.66666625
GTAATAC551.8990164E-523.5454543
GACGGTT551.8990164E-523.54545429
CGAACTA802.7155693E-823.12529
GCGAACT802.7155693E-823.12528
CGCCAAT400.001929203523.1251
CATGCTA855.1613824E-821.7647064
TGTAATA603.7192036E-521.5833342
AACTACT957.1122486E-921.42105331
CTTACCT3150.021.14285729
CTAGAGA450.00382191620.5555554
TTAGACA450.00382191620.5555554
GAAAGCG450.00382191620.55555534
AGCGCCG450.00382191620.55555532