FastQCFastQC Report
Fri 10 Feb 2017
ERR1633019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174688
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3650.20894394577761496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.14253984246198936No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2140.1225041216339989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1880.10762044330463454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTT251.2293433E-437.014
ACGTGAT251.2293433E-437.037
GCCGTTA251.2293433E-437.015
CCGTTAA303.5871012E-430.83333416
TAACCTG250.00548374729.65
CCTACGT405.911768E-527.75000234
GGTACCG358.8391855E-426.4285726
TAAAGTC358.8391855E-426.42857228
CGGCCGT358.8391855E-426.42857213
AGGGTAC358.8391855E-426.4285724
AAGCCGA358.8391855E-426.42857236
TAAGCCG358.8391855E-426.42857235
TACGTGA358.8391855E-426.42857236
GGTATCA856.91216E-1126.1176471
CATGCTA809.640644E-1025.4375024
ACGATTA451.3169459E-424.66666623
GTACATG1150.024.1304321
CGAACTA851.9481377E-923.94117729
GCGAACT851.9481377E-923.94117728
AAAGCGA851.9481377E-923.94117725
Produced by FastQC (version 0.11.5)