##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174688
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.088443396226417	33.0	33.0	33.0	14.0	33.0
2	30.140341637662576	33.0	33.0	33.0	14.0	33.0
3	30.62178283568419	33.0	33.0	33.0	27.0	33.0
4	30.769629281919766	33.0	33.0	33.0	27.0	33.0
5	30.91069220553215	33.0	33.0	33.0	27.0	33.0
6	32.701004075838064	37.0	33.0	37.0	14.0	37.0
7	32.95122733101301	37.0	33.0	37.0	14.0	37.0
8	33.108003984246196	37.0	33.0	37.0	14.0	37.0
9	33.00681214508152	37.0	33.0	37.0	14.0	37.0
10	33.06674757281554	37.0	33.0	37.0	14.0	37.0
11	33.032515112658	37.0	33.0	37.0	14.0	37.0
12	33.23192205532149	37.0	33.0	37.0	22.0	37.0
13	33.2393581699945	37.0	33.0	37.0	27.0	37.0
14	33.183876396775965	37.0	33.0	37.0	14.0	37.0
15	33.3259983513464	37.0	33.0	37.0	27.0	37.0
16	33.258695502839345	37.0	33.0	37.0	27.0	37.0
17	33.30655225315992	37.0	33.0	37.0	27.0	37.0
18	33.278084356109176	37.0	33.0	37.0	22.0	37.0
19	33.31767494046529	37.0	33.0	37.0	27.0	37.0
20	33.22481796116505	37.0	33.0	37.0	14.0	37.0
21	33.318899981681625	37.0	33.0	37.0	27.0	37.0
22	33.318722522440005	37.0	33.0	37.0	27.0	37.0
23	33.27208508884411	37.0	33.0	37.0	22.0	37.0
24	33.24870626488368	37.0	33.0	37.0	22.0	37.0
25	33.28694586920682	37.0	33.0	37.0	22.0	37.0
26	33.20130174940465	37.0	33.0	37.0	14.0	37.0
27	33.113842965744645	37.0	33.0	37.0	14.0	37.0
28	33.077807290712585	37.0	33.0	37.0	14.0	37.0
29	33.15493336691702	37.0	33.0	37.0	14.0	37.0
30	33.08641692617696	37.0	33.0	37.0	14.0	37.0
31	33.07954181168712	37.0	33.0	37.0	14.0	37.0
32	33.10177001282286	37.0	33.0	37.0	14.0	37.0
33	33.070525737314526	37.0	33.0	37.0	14.0	37.0
34	32.96720438725041	37.0	33.0	37.0	14.0	37.0
35	32.99422971240154	37.0	33.0	37.0	14.0	37.0
36	32.971560725407585	37.0	33.0	37.0	14.0	37.0
37	33.00267333760762	37.0	33.0	37.0	14.0	37.0
38	32.88592233009709	37.0	33.0	37.0	14.0	37.0
39	32.96433069243451	37.0	33.0	37.0	14.0	37.0
40	32.968412254991755	37.0	33.0	37.0	14.0	37.0
41	33.04554978017952	37.0	33.0	37.0	14.0	37.0
42	32.83943945777615	37.0	33.0	37.0	14.0	37.0
43	32.52037346583624	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	18.0
17	13.0
18	19.0
19	58.0
20	140.0
21	364.0
22	721.0
23	1318.0
24	2258.0
25	3305.0
26	4562.0
27	6048.0
28	7490.0
29	9093.0
30	10775.0
31	12727.0
32	15039.0
33	18192.0
34	22813.0
35	30574.0
36	29154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.777271478292725	22.327807290712585	12.102147829272761	24.792773401721927
2	17.100201502106614	24.572380472614032	34.75682359406485	23.57059443121451
3	18.993290895768457	28.24922146913354	29.072975819747203	23.6845118153508
4	14.477239421139403	20.467919948708555	37.121038651767726	27.93380197838432
5	15.734337790804176	36.88061000183184	34.73793277156989	12.647119435794101
6	29.620809672101117	40.442388715882025	17.121954570434145	12.814847041582707
7	26.94403736948159	32.13386151309764	23.722865909507235	17.19923520791354
8	24.260395676863894	36.557748671917935	20.319655614581425	18.862200036636747
9	25.789407400622828	15.761242901630334	19.94355651218172	38.505793185565125
10	15.043964095988278	28.37745008243268	34.265662209195824	22.31292361238322
11	34.401332661659644	23.838500641143067	23.93409965195091	17.826067045246383
12	22.84816358307382	25.93423703975087	30.249358856933505	20.968240520241803
13	27.96242443671002	22.32151034988093	25.91878091225499	23.797284301154058
14	21.875572449166512	21.03750686939	27.51305184099652	29.57386884044697
15	23.91291903278989	29.061526836416927	23.985047627770655	23.04050650302253
16	23.295246382121267	28.34653782744092	24.926154057519692	23.432061732918115
17	22.319793002381388	28.17652042498626	26.210157538010627	23.293529034621727
18	23.094889173841363	27.70882945594431	27.031622091958234	22.164659278256092
19	24.49052024180253	26.665827074555782	26.938885326982962	21.904767356658727
20	23.906622091958234	27.042498626122004	27.069976186114676	21.980903095805093
21	23.019325883861512	27.278347682725773	27.128366001099103	22.573960432313612
22	22.900828906393112	27.158705806924345	26.937167979483423	23.003297307199123
23	23.029629968858764	26.855880197838434	27.641280454295657	22.473209379007145
24	22.932886059717898	27.793551932588382	25.93996153141601	23.33360047627771
25	23.23456677047078	27.141532331928925	27.20335684191244	22.420544055687856
26	23.202509617145996	27.445502839347867	27.16614764608903	22.185839897417107
27	23.00615955303169	27.32643341271295	26.371015753801064	23.296391280454294
28	22.642654332295294	27.138670086096354	27.86968767173475	22.348987909873603
29	24.14018135189595	26.824967942846673	26.645218904561276	22.389631800696097
30	23.451525004579594	26.75455669536545	27.061961897783476	22.73195640227148
31	23.31814434878183	27.080280271111924	26.95090675947976	22.650668620626487
32	22.549345118153507	26.8913720461623	26.917704707821944	23.641578127862246
33	22.630060450631984	26.900531232826523	27.645287598461255	22.824120718080234
34	23.4354964279172	26.45173108627954	27.115772119435793	22.997000366367466
35	22.932886059717898	27.36421505770288	27.15813335775783	22.544765524821393
36	24.02626396775966	27.091729254442203	26.21416468217622	22.66784209562191
37	23.170452463821213	26.672696464553947	26.897668986993956	23.259182084630886
38	23.155568785491848	27.12951089943213	26.885075105330646	22.829845209745375
39	22.778324784759114	26.9159873603224	27.288651767723028	23.017036087195457
40	22.832707455577943	27.478132441839165	27.339599743542774	22.349560359040115
41	22.886517677230263	26.384182084630886	27.272050741894123	23.457249496244735
42	22.686732918116874	27.329868107712034	27.135807840263787	22.847591133907308
43	23.559717897050742	26.272554497160648	27.066541491115586	23.101186114673016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	18.5
4	33.0
5	33.0
6	47.5
7	62.0
8	61.0
9	60.0
10	84.5
11	109.0
12	109.0
13	165.5
14	222.0
15	366.0
16	510.0
17	613.0
18	716.0
19	716.0
20	729.0
21	742.0
22	864.0
23	986.0
24	1343.5
25	1701.0
26	1701.0
27	2279.5
28	2858.0
29	3661.5
30	4465.0
31	5552.0
32	6639.0
33	6639.0
34	8004.0
35	9369.0
36	10513.5
37	11658.0
38	12549.5
39	13441.0
40	13441.0
41	14354.5
42	15268.0
43	15520.0
44	15772.0
45	15257.5
46	14743.0
47	14743.0
48	14308.5
49	13874.0
50	13587.5
51	13301.0
52	12321.0
53	11341.0
54	11341.0
55	10834.5
56	10328.0
57	8843.5
58	7359.0
59	6761.0
60	6163.0
61	6163.0
62	5467.0
63	4771.0
64	3888.0
65	3005.0
66	2504.0
67	2003.0
68	2003.0
69	1625.5
70	1248.0
71	1036.0
72	824.0
73	643.0
74	462.0
75	462.0
76	363.5
77	265.0
78	213.5
79	162.0
80	126.5
81	91.0
82	91.0
83	74.5
84	58.0
85	47.5
86	37.0
87	31.5
88	26.0
89	26.0
90	18.0
91	10.0
92	5.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	174688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.18904589781907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36645963571381	57.605251930203224
2	5.8296826751261674	7.600629029570408
3	1.8035545432891185	3.5271599970508336
4	0.9311278396406414	2.4279734190028353
5	0.592800441254595	1.9322047586596593
6	0.4001071522265434	1.564956210632509
7	0.27836773851575736	1.270256909780306
8	0.23845657367783255	1.2435805220896718
9	0.18158591348081973	1.0653671203468706
>10	1.2800804903682348	16.082615302888755
>50	0.07383160975795798	3.289758877506187
>100	0.02394538694852691	2.39024592226873
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	365	0.20894394577761496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.14253984246198936	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	214	0.1225041216339989	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	188	0.10762044330463454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.724491665140136E-4	0.0	0.0
2	0.0	0.0	5.724491665140136E-4	0.0	0.0
3	0.0	0.0	5.724491665140136E-4	0.0	0.0
4	0.0	0.0	0.0022897966660560543	0.0	0.0
5	0.0	0.0	0.0022897966660560543	0.0	0.0
6	0.0	0.0	0.002862245832570068	0.0	0.0
7	0.0	0.0	0.0045795933321121085	0.0	0.0
8	0.0	0.0	0.0045795933321121085	0.0	0.0
9	0.0	0.0	0.0045795933321121085	0.0	0.0
10	5.724491665140136E-4	0.0	0.005152042498626122	0.0	0.0
11	5.724491665140136E-4	0.0	0.008586737497710203	0.0	0.0
12	5.724491665140136E-4	0.0	0.010304084997252244	0.0	0.0
13	5.724491665140136E-4	0.0	0.010876534163766258	0.0	0.0
14	5.724491665140136E-4	0.0	0.010876534163766258	0.0	0.0
15	5.724491665140136E-4	0.0	0.012593881663308298	0.0	0.0
16	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
17	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
18	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
19	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
20	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
21	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
22	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
23	5.724491665140136E-4	0.0	0.013166330829822312	0.0	0.0
24	5.724491665140136E-4	0.0	0.013738779996336326	0.0	0.0
25	5.724491665140136E-4	0.0	0.013738779996336326	0.0	0.0
26	5.724491665140136E-4	0.0	0.013738779996336326	5.724491665140136E-4	0.0
27	5.724491665140136E-4	0.0	0.013738779996336326	0.0011448983330280271	0.0
28	5.724491665140136E-4	0.0	0.013738779996336326	0.0034346949990840814	0.0
29	5.724491665140136E-4	0.0	0.013738779996336326	0.00801428833119619	0.0
30	5.724491665140136E-4	0.0	0.013738779996336326	0.021753068327532515	0.0
31	5.724491665140136E-4	0.0	0.013738779996336326	0.06525920498259755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTT	25	1.2293433E-4	37.0	14
ACGTGAT	25	1.2293433E-4	37.0	37
GCCGTTA	25	1.2293433E-4	37.0	15
CCGTTAA	30	3.5871012E-4	30.833334	16
TAACCTG	25	0.005483747	29.6	5
CCTACGT	40	5.911768E-5	27.750002	34
GGTACCG	35	8.8391855E-4	26.428572	6
TAAAGTC	35	8.8391855E-4	26.428572	28
CGGCCGT	35	8.8391855E-4	26.428572	13
AGGGTAC	35	8.8391855E-4	26.428572	4
AAGCCGA	35	8.8391855E-4	26.428572	36
TAAGCCG	35	8.8391855E-4	26.428572	35
TACGTGA	35	8.8391855E-4	26.428572	36
GGTATCA	85	6.91216E-11	26.117647	1
CATGCTA	80	9.640644E-10	25.437502	4
ACGATTA	45	1.3169459E-4	24.666666	23
GTACATG	115	0.0	24.130432	1
CGAACTA	85	1.9481377E-9	23.941177	29
GCGAACT	85	1.9481377E-9	23.941177	28
AAAGCGA	85	1.9481377E-9	23.941177	25
>>END_MODULE